CAJ | 학술논문

目的：应用聚合酶链反应（PCR）-变性梯度凝胶电泳（DGGE）技术，分析30对母子口腔唾液中菌群的相似性。方法???选取3~5岁高龋、中龋和无龋儿童及其母亲各10对作为研究对象，取其非刺激性全唾液，分别提取细菌总DNA，进行16S?rRNA的PCR扩增及DGGE分析。结果???母子间口腔菌群组成具高度相似性。其中，无龋组母子间配对相似系数明显低于高龋组及中龋组（P<0.05）；高龋儿童的母亲平均龋失补牙面数（DMFS）明显高于中龋和无龋儿童的母亲（P<0.05）。结论母亲是儿童口腔菌群获得的主要来源。
목적：응용취합매련반응（PCR）-변성제도응효전영（DGGE）기술，분석30대모자구강타액중균군적상사성。방법???선취3~5세고우、중우화무우인동급기모친각10대작위연구대상，취기비자격성전타액，분별제취세균총DNA，진행16S?rRNA적PCR확증급DGGE분석。결과???모자간구강균군조성구고도상사성。기중，무우조모자간배대상사계수명현저우고우조급중우조（P<0.05）；고우인동적모친평균우실보아면수（DMFS）명현고우중우화무우인동적모친（P<0.05）。결론모친시인동구강균군획득적주요래원。
Objective??This?study?aimed?to?analyze?the?similarity?of?the?oral?bacteria?community?in?30?mother-child?dyads?via?polymerase?chain?reaction(PCR)-based?denaturing?gradient?gel?electrophoresis.?Methods??Unstimulated?saliva?samples?were?obtained?from?30?mother-child?pairs:?10?in?caries-susceptive?group,?10?in?caries-moderate?group,?and?10?in?caries-free?group.?DNAs?were?extracted?for?PCR?amplification?and?denaturing?gradient?gel?electrophoresis?analysis.?Results???The?compositions?of?the?oral?bacteria?community?in?mother?and?child?have?a?high?degree?of?similarity.?The?oral?bacteria?community?of?children?matched?that?of?their?mothers.?The?dice?similarity?coefficient?of?mother-child?in?the?caries-free group was more statistically significant compared with the two other groups(P<0.05).?However,?mothers?of?caries-susceptive?children?had?significantly?higher?decay?missing?filling?surface(DMFS)?scores?than?mothers?of?caries-free?and?caries-moderate?children(P<0.05).?Conclusion???Children?obtained?oral?bacteria?from?their?mothers.