CAJ | 학술논문

目的：探讨单壁碳纳米管对人肝癌HepG2细胞的体外毒性和氧化损伤作用。方法选择人肝癌HepG2细胞，分为设置0、1.5、3、6、12和24mg/L 5个浓度的单壁碳纳米管，细胞经单壁碳纳米管分别孵育24、48h后，分析单壁碳纳米管的细胞毒性和氧化损伤通过检测线粒体膜电位和细胞活性氧(ROS)的变化情况；采用流式细胞术(FCM)检测单壁碳纳米管影响细胞凋亡情况。结果随着单壁碳纳米管孵育剂量的增加，HepG2细胞的膜电位受损，ROS含量逐渐增加，细胞凋亡率逐渐升高。结论单壁碳纳米管可以对HepG2细胞产生毒性，并且毒性效应随着孵育浓度的增加而增加，判断氧化损伤可能是单壁碳纳米管毒性作用机制组成部分。
목적：탐토단벽탄납미관대인간암HepG2세포적체외독성화양화손상작용。방법선택인간암HepG2세포，분위설치0、1.5、3、6、12화24mg/L 5개농도적단벽탄납미관，세포경단벽탄납미관분별부육24、48h후，분석단벽탄납미관적세포독성화양화손상통과검측선립체막전위화세포활성양(ROS)적변화정황；채용류식세포술(FCM)검측단벽탄납미관영향세포조망정황。결과수착단벽탄납미관부육제량적증가，HepG2세포적막전위수손，ROS함량축점증가，세포조망솔축점승고。결론단벽탄납미관가이대HepG2세포산생독성，병차독성효응수착부육농도적증가이증가，판단양화손상가능시단벽탄납미관독성작용궤제조성부분。
Objective To investigate the single-walled carbon nanotubes on human hepatocellular carcinoma HepG2 cells in vitro toxicity and oxidative damage. Methods Human hepatocellular carcinoma HepG2 cells,divided into set 0,1.5,3,6,12 and 24mg/L five concentrations of single-walled carbon nanotubes,cells of single-walled carbon nanotubes were incubated with 24, 48h,after analysis of the cytotoxicity of single-walled carbon nanotubes and oxidative damage by detecting the mitochondrial membrane potential and cellular reactive oxygen species (ROS) changes;Using flow cytometry (FCM) single-walled carbon nan-otubes affect cell apoptosis. Results With the increase of incubation dose of single-walled carbon nanotubes,HepG2 cell mem-brane potential damage,ROS content gradually increased,cell apoptosis rate increased. Conclusion Single-walled carbon nan-otubes can be generated on HepG2 cell toxicity,and toxic effect increases with the increase of incubation,judgment,oxidative damage may be toxic mechanism of single-walled carbon nanotubes.