中华核医学与分子影像杂志
中華覈醫學與分子影像雜誌
중화핵의학여분자영상잡지
Chinese Journal of Nuclear Medicine and Molecular Imaging
2015年
5期
355-358
,共4页
贾红丽%冯珏%张秀梅%方凤宁%杨阳%张自伟
賈紅麗%馮玨%張秀梅%方鳳寧%楊暘%張自偉
가홍려%풍각%장수매%방봉저%양양%장자위
肝肿瘤%胞嘧啶%寡脱氧核糖核苷酸类,反义%锝%体层摄影术,发射型计算机,单光子%小鼠,裸
肝腫瘤%胞嘧啶%寡脫氧覈糖覈苷痠類,反義%锝%體層攝影術,髮射型計算機,單光子%小鼠,裸
간종류%포밀정%과탈양핵당핵감산류,반의%득%체층섭영술,발사형계산궤,단광자%소서,라
Liver neoplasms%Cytosine%Oligodeoxyribonucleotides,antisense%Technetium%Tomography,emission-computed,single-photon%Mice,nude
目的 研究胞嘧啶(C)含量对反义探针肿瘤特异性聚集的影响,为最佳反义显像碱基序列的筛选提供帮助.方法 以survivin为靶序列制备3种(A1、A2、A3)C含量不同(10％、20％、30％)的20个碱基单链ASODN,耦联HYNIC,经99 Tcm标记、Cellufine GH-25纯化、脂质体包裹后获得3种标记化合物.分别对荷肝癌裸鼠行反义显像,各组计量资料比较采用Kruskal-Wallis H检验.结果 注射后4h,3种标记化合物在荷肝癌裸鼠体内均表现为肝、肾高摄取.随着C含量升高,3种标记化合物的肾脏摄取依次升高,分别为(1.50±0.06)、(2.80±0.09)和(3.96±0.03) ％ID/g;肿瘤摄取依次降低,分别为(2.08±0.08)、(1.69±0.01)和(1.20±0.09) ％ID/g;体内显像的T/NT比值分别为4.49～4.93、4.12～4.21和3.35～ 3.85,差异有统计学意义(H=12.50,P＜0.05);生物分布的T/NT比值分别为4.08 ～ 4.94、4.02～4.18和3.66～3.85,差异有统计学意义(H=10.82,P＜0.05).结论 Survivin ASODN中的C含量越低,标记化合物的肾内滞留率越低,肿瘤特异性结合率越高,显像质量越好.
目的 研究胞嘧啶(C)含量對反義探針腫瘤特異性聚集的影響,為最佳反義顯像堿基序列的篩選提供幫助.方法 以survivin為靶序列製備3種(A1、A2、A3)C含量不同(10％、20％、30％)的20箇堿基單鏈ASODN,耦聯HYNIC,經99 Tcm標記、Cellufine GH-25純化、脂質體包裹後穫得3種標記化閤物.分彆對荷肝癌裸鼠行反義顯像,各組計量資料比較採用Kruskal-Wallis H檢驗.結果 註射後4h,3種標記化閤物在荷肝癌裸鼠體內均錶現為肝、腎高攝取.隨著C含量升高,3種標記化閤物的腎髒攝取依次升高,分彆為(1.50±0.06)、(2.80±0.09)和(3.96±0.03) ％ID/g;腫瘤攝取依次降低,分彆為(2.08±0.08)、(1.69±0.01)和(1.20±0.09) ％ID/g;體內顯像的T/NT比值分彆為4.49～4.93、4.12～4.21和3.35～ 3.85,差異有統計學意義(H=12.50,P＜0.05);生物分佈的T/NT比值分彆為4.08 ～ 4.94、4.02～4.18和3.66～3.85,差異有統計學意義(H=10.82,P＜0.05).結論 Survivin ASODN中的C含量越低,標記化閤物的腎內滯留率越低,腫瘤特異性結閤率越高,顯像質量越好.
목적 연구포밀정(C)함량대반의탐침종류특이성취집적영향,위최가반의현상감기서렬적사선제공방조.방법 이survivin위파서렬제비3충(A1、A2、A3)C함량불동(10％、20％、30％)적20개감기단련ASODN,우련HYNIC,경99 Tcm표기、Cellufine GH-25순화、지질체포과후획득3충표기화합물.분별대하간암라서행반의현상,각조계량자료비교채용Kruskal-Wallis H검험.결과 주사후4h,3충표기화합물재하간암라서체내균표현위간、신고섭취.수착C함량승고,3충표기화합물적신장섭취의차승고,분별위(1.50±0.06)、(2.80±0.09)화(3.96±0.03) ％ID/g;종류섭취의차강저,분별위(2.08±0.08)、(1.69±0.01)화(1.20±0.09) ％ID/g;체내현상적T/NT비치분별위4.49～4.93、4.12～4.21화3.35～ 3.85,차이유통계학의의(H=12.50,P＜0.05);생물분포적T/NT비치분별위4.08 ～ 4.94、4.02～4.18화3.66～3.85,차이유통계학의의(H=10.82,P＜0.05).결론 Survivin ASODN중적C함량월저,표기화합물적신내체류솔월저,종류특이성결합솔월고,현상질량월호.
Objective To investigate whether specific cellular uptake of 99Tcm-survivin-ASODN in nude mice bearing human HCC is influenced by its cytosine content.Methods Three kinds(A1,A2,A3) of synthesized survivin ASODN with three cytosine contents(10％,20％,30％),20 bases per single-strand were prepared.They were labeled with 99Tcm by conjugating with a bifunctional chelator HYNIC,purified through Cellufine GH-25 and then encapsulated with liposome.Antisense gene imaging and the biodistribution of 99Tcm-HYNIC-survivin ASODN in nude mice bearing HCC were performed.The data were analysed by Kruskal-Wallis H test.Results At 4 h post injection,all the 3 labelled compounds showed increased uptake by tumor,liver and kidney.With increase in cytosine content,the uptake increased in kidney (％ID/g:1.50±0.06,2.80±0.09 and 3.96±0.03),and decreased in tumor (％ID/g:2.08±0.08,1.69±0.01 and 1.20±0.09).T/NT in imaging (4.49-4.93,4.12-4.21,3.35-3.85;H=12.50,P＜0.05) and in biodistribution (4.08-4.94,4.02-4.18,3.66-3.85;H=10.82,P＜0.05) were all significantly different.Conclusion ASODN with lower cytosine content shows higher uptake by HCC tumor cells and less stasis in kidneys,thus providing better quality in antisense gene imaging.
