CAJ | 학술논문

目的检测端粒保护蛋白POT1(protection of telomeres 1) mRNA和TPP1(POT1-interacting protein 1)mRNA在膀胱尿路上皮癌中的表达. 分析两者表达的相关性及与膀胱尿路上皮癌的病理分级、临床分期之间的关系. 方法收集32例膀胱尿路上皮癌组织标本,17例癌旁组织标本,12例正常膀胱粘膜组织标本. 提取各组标本的总RNA,采用实时荧光定量聚合酶链反应( real-time quantitative polymerase chain reaction,RT-qPCR)检测端粒保护蛋白POT1 mRNA和TPP1 mRNA在各组标本中的表达. 结果 POT 1 mRNA、TPP 1 mRNA在膀胱尿路上皮癌组织组中的相对表达量,均低于其在癌旁组织组中的相对表达量,组间差异有统计学意义(P<0. 01);且均低于其在正常膀胱粘膜组织组中的相对表达量,组间差异有统计学意义(P<0. 01);而POT 1 mRNA、TPP 1 mRNA的相对表达量在癌旁组织组与正常膀胱粘膜组织组相比较,组间差异无统计学意义. 在各组标本中,POT 1 mRNA与TPP 1 mRNA相对表达量之间成线性相关. 膀胱尿路上皮癌组织中POT1 mRNA、 TPP 1 mRNA 的相对表达量在各病理分级间差异无统计学意义,而膀胱尿路上皮癌组织中POT1mRNA、TPP 1mRNA的相对表达量在临床分期间的差异有统计学意义,并且随着临床分期的增加而升高. 结论端粒保护蛋白POT1 mRNA和TPP1 mRNA在膀胱尿路上皮癌组织中的表达低于癌旁组织及正常粘膜组织. POT1 mRNA和TPP1 mRNA的表达之间线性相关. 端粒保护蛋白POT1和TPP1的表达可能与膀胱肿瘤的发生及病理分级、临床分期存在相关性.
목적 검측단립보호단백POT1(protection of telomeres 1) mRNA화TPP1(POT1-interacting protein 1)mRNA재방광뇨로상피암중적표체. 분석량자표체적상관성급여방광뇨로상피암적병리분급、림상분기지간적관계. 방법 수집32례방광뇨로상피암조직표본,17례암방조직표본,12례정상방광점막조직표본. 제취각조표본적총RNA,채용실시형광정량취합매련반응( real-time quantitative polymerase chain reaction,RT-qPCR)검측단립보호단백POT1 mRNA화TPP1 mRNA재각조표본중적표체. 결과 POT 1 mRNA、TPP 1 mRNA재방광뇨로상피암조직조중적상대표체량,균저우기재암방조직조중적상대표체량,조간차이유통계학의의(P<0. 01);차균저우기재정상방광점막조직조중적상대표체량,조간차이유통계학의의(P<0. 01);이POT 1 mRNA、TPP 1 mRNA적상대표체량재암방조직조여정상방광점막조직조상비교,조간차이무통계학의의. 재각조표본중,POT 1 mRNA여TPP 1 mRNA상대표체량지간성선성상관. 방광뇨로상피암조직중POT1 mRNA、 TPP 1 mRNA 적상대표체량재각병리분급간차이무통계학의의,이방광뇨로상피암조직중POT1mRNA、TPP 1mRNA적상대표체량재림상분기간적차이유통계학의의,병차수착림상분기적증가이승고. 결론 단립보호단백POT1 mRNA화TPP1 mRNA재방광뇨로상피암조직중적표체저우암방조직급정상점막조직. POT1 mRNA화TPP1 mRNA적표체지간선성상관. 단립보호단백POT1화TPP1적표체가능여방광종류적발생급병리분급、림상분기존재상관성.
Objective To detect the expression of telomeric protein POT1 ( protection of telomeres 1 ) mRNA and TPP1 (POT1-interacting protein 1) mRNA in bladder urothelial carcinoma. Analysis of the correlation between the expression of the two telomeric protein mRNAs and the correlation between the pathological grade or clinical stage in bladder urothelial carcinoma. Me-theods The 32 cases of bladder urothelial carcinoma specimens were collected from bladder cancer surgery patients;the 17 cases of adjacent tissues specimens were collected;and the 12 cases of normal bladder mucosa specimens were collected. The experiment was divided into three groups:the group of bladder urothelial carcinoma ,the group of adjacent tissues and normal bladder mucosa tissue group. Total RNA of all specimens of each group were extracted. The expression of POT1 mRNA and TPP1 mRNA in each specimens were detected using real-time quantitative polymerase chain reaction ( RT-qPCR ) . Results The relative amount of POT1 mRNA expression was lower than adjacent tissues,the difference between the groups was statistically significant(P<0. 01);which was lower than that in normal bladder mucosa tissues, the difference between the groups was statistically significant ( P<0. 01);Compared the relative expression level of POT 1 mRNA in adjacent tissues with that in normal bladder mucosa tissues,the difference between the groups was not statistically significant. Between the relative expression of relative POT1 mRNA and TPP1 mRNA were into a linear correlation. The difference of the relative expression levels of POT1 and TPP 1 mRNAs in bladder urothe-lial carcinoma with pathological grade was no statistically significant;the difference of the relative expression levels of POT1 and TPP 1 mRNAs in bladder urothelial carcinoma with Clinical stage was statistically significant,and the relative expression levels of POT1mRNA and TPP 1mRNA increased with the increase in clinical stage. Conclusion The expression level of telomeric protein POT1 mRNA and TPP1 mRNA in bladder urothelial carcinoma tissues were lower than that in adjacent tissues and normal mucosal tissues. Between the relative expression of relative POT1 mRNA and TPP1 mRNA were into a linear correlation. The reduction of POT1 mRNA and TPP1 mRNA in bladder urothelial carcinoma tissues may be associated with the occurrence of bladder tumors and correlated with the pathological grade or clinical stage in bladder urothelial carcinoma.