CAJ | 학술논문

目的研究CXCL14在人子宫内膜中的时序性表达,探讨其与子宫内膜容受性形成的关系. 方法应用Real-time PCR法检测18例正常妇女月经周期不同时期子宫内膜中CXCL14 mRNA的表达变化,应用冰冻切片免疫荧光的方法检测CXCL14蛋白水平的表达及组织分布,观察CXCL14 表达变化与着床窗开放的关系. 结果 CXCL14 mRNA和蛋白表达变化一致,在分泌中晚期子宫内膜中表达增高,在分泌中期达顶峰. CXCL14蛋白在子宫内膜上皮和基质中均有分布,主要分布在子宫内膜腺上皮和腔上皮中,与着床窗的开放时间一致. 结论 CXCL14在子宫内膜容受窗期特异性高表达,可能参与了子宫内膜容受性的形成.
목적 연구CXCL14재인자궁내막중적시서성표체,탐토기여자궁내막용수성형성적관계. 방법 응용Real-time PCR법검측18례정상부녀월경주기불동시기자궁내막중CXCL14 mRNA적표체변화,응용빙동절편면역형광적방법검측CXCL14단백수평적표체급조직분포,관찰CXCL14 표체변화여착상창개방적관계. 결과 CXCL14 mRNA화단백표체변화일치,재분비중만기자궁내막중표체증고,재분비중기체정봉. CXCL14단백재자궁내막상피화기질중균유분포,주요분포재자궁내막선상피화강상피중,여착상창적개방시간일치. 결론 CXCL14재자궁내막용수창기특이성고표체,가능삼여료자궁내막용수성적형성.
Objective To study the expression pattern of CXCL14 in human endometrium and its relationship with endo-metrial receptivity. Methods A total of 18 samples were collected from normal fertile women of menstrual cycle in different peri-ods. The mRNA expression of CXCL14 was examined by real-time PCR analysis,and the CXCL14 protein expression and distribu-tion were conducted by frozen section and immunofluorescence analysis. Results We found that the expression of CXCL14 mRNA was markedly increased in the mid-and late-secretory phase of endometrium,and peaked in mid-secretory phase,which consistent with the changes of CXCL14 protein expression. The tissue immunofluorescence analysis revealed that CXCL14 signals were ob-served in the glandular and luminal epithelium cells as well as stromal cells,but mainly localized in the glandular and luminal epi-thelium,consistent with the implantation window opening time. Conclusion CXCL14 was highly up-regulated in the mid-secretory phase,which consistent with the open of implantation window,indicated that CXCL14 may play a role in the establishment of endo-metrial receptivity.