中国骨质疏松杂志
中國骨質疏鬆雜誌
중국골질소송잡지
Chinese Journal of Osteoporosis
2015年
10期
1195-1200
,共6页
沈耿杨%任辉%梁德%江晓兵%姚珍松%杨志东%魏秋实%唐晶晶%崔健超%林顺鑫
瀋耿楊%任輝%樑德%江曉兵%姚珍鬆%楊誌東%魏鞦實%唐晶晶%崔健超%林順鑫
침경양%임휘%량덕%강효병%요진송%양지동%위추실%당정정%최건초%림순흠
激素%去卵巢%骨质疏松症%差异比较%机制
激素%去卵巢%骨質疏鬆癥%差異比較%機製
격소%거란소%골질소송증%차이비교%궤제
Glucocorticoid%Ovariectomy%Osteoporosis%Difference comparison%Mechanism
目的：比较激素及去卵巢对大鼠骨量、骨转换指标、雌激素水平、骨髓细胞成脂分化水平的影响，并探讨两种模型可能的分子机制。方法3月龄雌性SD大鼠36只，随机分为空白组、甲强龙组（ MP组）、去卵巢组（ OVX组）。3组大鼠于8w后处死，测量体重及子宫、肾上腺重量；双能X线骨密度仪测量左股骨BMD、BMC、BA；ELISA法测量血清PINP、β?CTX及雌激素水平；油红“O”染色观察右股骨骨髓间充质干细胞（ BMSCs）成脂情况。 RT?PCR检测左胫骨Runx2、Col1α1、MMP9 mRNA表达。结果术后8w，MP组体重显著低于空白组及OVX组，OVX组体重明显高于空白组（P＜0?05）。 MP组BMC、BMD、BA及OVX组BMC、BMD均较空白组明显降低，且MP组BMC、BMD、BA明显低于OVX组（ P＜0?05）。两组血清PINP、β?CTX均较空白组显著升高，且MP组明显高于OVX组，两组雌激素水平均显著低于空白组（P＜0?05）。两组骨髓脂滴均较空白组增多，骨组织Runx2 mRNA表达均较空白组显著下调（P＜0?05），Col1α1、MMP9 mRNA表达较空白组高，但比较差异无统计学意义。结论激素及去卵巢均可诱导大鼠高转换型骨质疏松症，以激素导致的骨量丢失更严重，且两者均可下调大鼠血清雌激素水平、促进骨髓细胞成脂分化，其机制可能均与调控骨组织Runx2 mRNA表达水平密切相关。
目的：比較激素及去卵巢對大鼠骨量、骨轉換指標、雌激素水平、骨髓細胞成脂分化水平的影響，併探討兩種模型可能的分子機製。方法3月齡雌性SD大鼠36隻，隨機分為空白組、甲彊龍組（ MP組）、去卵巢組（ OVX組）。3組大鼠于8w後處死，測量體重及子宮、腎上腺重量；雙能X線骨密度儀測量左股骨BMD、BMC、BA；ELISA法測量血清PINP、β?CTX及雌激素水平；油紅“O”染色觀察右股骨骨髓間充質榦細胞（ BMSCs）成脂情況。 RT?PCR檢測左脛骨Runx2、Col1α1、MMP9 mRNA錶達。結果術後8w，MP組體重顯著低于空白組及OVX組，OVX組體重明顯高于空白組（P＜0?05）。 MP組BMC、BMD、BA及OVX組BMC、BMD均較空白組明顯降低，且MP組BMC、BMD、BA明顯低于OVX組（ P＜0?05）。兩組血清PINP、β?CTX均較空白組顯著升高，且MP組明顯高于OVX組，兩組雌激素水平均顯著低于空白組（P＜0?05）。兩組骨髓脂滴均較空白組增多，骨組織Runx2 mRNA錶達均較空白組顯著下調（P＜0?05），Col1α1、MMP9 mRNA錶達較空白組高，但比較差異無統計學意義。結論激素及去卵巢均可誘導大鼠高轉換型骨質疏鬆癥，以激素導緻的骨量丟失更嚴重，且兩者均可下調大鼠血清雌激素水平、促進骨髓細胞成脂分化，其機製可能均與調控骨組織Runx2 mRNA錶達水平密切相關。
목적：비교격소급거란소대대서골량、골전환지표、자격소수평、골수세포성지분화수평적영향，병탐토량충모형가능적분자궤제。방법3월령자성SD대서36지，수궤분위공백조、갑강룡조（ MP조）、거란소조（ OVX조）。3조대서우8w후처사，측량체중급자궁、신상선중량；쌍능X선골밀도의측량좌고골BMD、BMC、BA；ELISA법측량혈청PINP、β?CTX급자격소수평；유홍“O”염색관찰우고골골수간충질간세포（ BMSCs）성지정황。 RT?PCR검측좌경골Runx2、Col1α1、MMP9 mRNA표체。결과술후8w，MP조체중현저저우공백조급OVX조，OVX조체중명현고우공백조（P＜0?05）。 MP조BMC、BMD、BA급OVX조BMC、BMD균교공백조명현강저，차MP조BMC、BMD、BA명현저우OVX조（ P＜0?05）。량조혈청PINP、β?CTX균교공백조현저승고，차MP조명현고우OVX조，량조자격소수평균현저저우공백조（P＜0?05）。량조골수지적균교공백조증다，골조직Runx2 mRNA표체균교공백조현저하조（P＜0?05），Col1α1、MMP9 mRNA표체교공백조고，단비교차이무통계학의의。결론격소급거란소균가유도대서고전환형골질소송증，이격소도치적골량주실경엄중，차량자균가하조대서혈청자격소수평、촉진골수세포성지분화，기궤제가능균여조공골조직Runx2 mRNA표체수평밀절상관。
Objective To compare the effect between glucocorticoid and ovariectomy on bone mass, bone turnover markers, estrogen level, and adipogenic differentiation by BMSCs, and to analyze the possible molecular mechanism. Methods Thirty six 3-month?old female Sprague Dawley rats were randomly divided to 3 groups, control group, methylprednisolone group ( MP) , and ovariectomized group ( OVX) . The rats in 3 groups were sacrificed in 8 weeks. The body mass, adrenal weight, and uterus weight were detected. BMD, BMC, and BA of the left femurs were detected using dual energy X?ray absorptiometry. The serum levels of PINP,β?CTX, and estrogen were determined using ELISA. Meanwhile, adipogenic differentiation by BMSCs was observed by oil red staining of the right femurs. The mRNA expression of Runx2, Col1α1, and MMP9 in the left tibia was detected using RT?PCR. Results In 8 weeks after the operation, the body mass in rats of MP group was significantly lower than that in control group and OVX group, while the body mass of OVX group was significantly higher than that in control group (P<0?05). BMC, BMD, and BA in rats of MP group and BMC and BMD in rats of OVX group were significantly lower than those in control group (P<0?05). In addition, BMC, BMD, and BA in MP group were significantly lower than those in OVX group (P<0?05). The levels of PINP and β?CTX in both groups were higher than those in control group, and they were significantly higher in MP group than in OVX group (P<0?05). The estrogen level in both groups were significantly lower than that in control group (P<0?05). The fat in bone marrow of both groups was more than that in control group. The expression of Runx2 mRNA in both groups was significantly down?regulated comparing with that in control group (P<0?05). The expression of Col1α1 and MMP9 mRNA was higher than that in control group, but the difference was not significant. Conclusion Both glucocorticoid and ovariectomy can induce high?turnover osteoporosis in rat, and glucocorticoid?induced bone loss is more severe. Both models can down?regulate estrogen level and promote adipogenic differentiation by BMSCs. The molecular mechanism may be related to the expression level of Runx2 mRNA.
