CAJ | 학술논문

目的：观察钙敏感受体( calcium sensing receptor, CaSR)在人胃癌SGC-7901细胞中的表达,对细胞内钙、增殖和迁移的影响。方法(1)采用Western blot及免疫荧光染色技术分析CaSR蛋白在人胃癌SGC-7901细胞的表达；采用激光共聚焦扫描技术检测CaSR活化对细胞内钙离子([ Ca2+] i )的影响；应用MTT法、流式细胞仪技术、细胞划痕实验检测CaSR活化对SGC-7901细胞增殖和迁移的影响。结果 CaSR蛋白在人胃癌SGC-7901细胞有表达；增加细胞外钙或者给予Calindol( CaSR激动剂)均可引起[ Ca2+] i、CaSR和E-cadherin的表达增加；CaSR活化对SGC-7901细胞的增殖无明显影响；细胞划痕实验结果显示细胞迁移能力下降。结论 CaSR在人胃癌SGC-7901细胞中有表达,并通过诱导E-cadherin的表达使SGC-7901细胞迁移能力降低。
목적：관찰개민감수체( calcium sensing receptor, CaSR)재인위암SGC-7901세포중적표체,대세포내개、증식화천이적영향。방법(1)채용Western blot급면역형광염색기술분석CaSR단백재인위암SGC-7901세포적표체；채용격광공취초소묘기술검측CaSR활화대세포내개리자([ Ca2+] i )적영향；응용MTT법、류식세포의기술、세포화흔실험검측CaSR활화대SGC-7901세포증식화천이적영향。결과 CaSR단백재인위암SGC-7901세포유표체；증가세포외개혹자급여Calindol( CaSR격동제)균가인기[ Ca2+] i、CaSR화E-cadherin적표체증가；CaSR활화대SGC-7901세포적증식무명현영향；세포화흔실험결과현시세포천이능력하강。결론 CaSR재인위암SGC-7901세포중유표체,병통과유도E-cadherin적표체사SGC-7901세포천이능력강저。
Purpose To observe the functional expression of calcium sensing receptor ( CaSR) in human gastric cancer SGC-7901 cell line, the effect of CaSR on intracellular calcium, cell proliferation and migration of SCG-7901. Methods The expression and distribu-tion of CaSR were detected by Western blotting and immunofluorescence observation in SGC-7901. The intracellular concentration of free calcium ( [ Ca2+] i ) was determined by confocal laser scanning microscopy. MTT, flow cytometry and scratch test were used to an-alyze the impact of CaSR the proliferation and the migration capabilities of SGC-7901 cell. Results CaSR protein was expressed in SGC-7901. Extracellular calcium or calindol significantly increased the expression of [Ca2+]i, CaSR and E-cadherin;In addition, the migration capabilities were decreased. Conclusion CaSR is expressed in SGC-7901. The activation of CaSR induces the expression of E-cadherin, and decreases migration ability.