临床与实验病理学杂志
臨床與實驗病理學雜誌
림상여실험병이학잡지
Chinese Journal of Clinical and Experimental Pathology
2015年
10期
1127-1130
,共4页
喉肿瘤%鳞状细胞癌%KAI1/CD82%CD44v6%免疫组织化学
喉腫瘤%鱗狀細胞癌%KAI1/CD82%CD44v6%免疫組織化學
후종류%린상세포암%KAI1/CD82%CD44v6%면역조직화학
laryngeal neoplasms%squamous cell carcinoma%KAI1/CD82%CD44v6%immunohistochemistry
目的：探讨肿瘤转移抑制基因KAI1/CD82和细胞分子CD44v6在人喉鳞状细胞癌( laryngeal squamous cell carcinoma, LSCC)中的表达及意义,并探讨二者之间的关系。方法采用免疫组化EnVision法检测64例LSCC和15例正常喉黏膜( nor-mal laryngeal mucosa, NLM)组织中KAI1/CD82和CD44v6的表达。结果 LSCC组织中KAI1/CD82蛋白的阳性率(37．50%)显著低于NLM组织(86．67%)(P=0．031)；LSCC组织中CD44v6蛋白的阳性率(75．00%)显著高于NLM组织(26．67%)(P=0．011)。 KAI1/CD82表达与LSCC临床分期、分化程度及淋巴结转移相关(P<0．05),与预后无关(P>0．05)；CD44v6表达与LSCC分化程度、淋巴结转移及预后相关(P<0．05),与临床分期无关(P>0．05)；二者均与患者年龄、性别无关(P>0．05)。KAI1/CD82与CD44v6在LSCC组织中的表达呈负相关(rs =-0．504,P=0．036)。结论 KAI1/CD82与CD44v6在LSCC的发生、发展和侵袭转移中起着相互抑制的作用,检测KAI1/CD82及CD44v6蛋白表达对判断LSCC的生物学行为具有重要价值。
目的：探討腫瘤轉移抑製基因KAI1/CD82和細胞分子CD44v6在人喉鱗狀細胞癌( laryngeal squamous cell carcinoma, LSCC)中的錶達及意義,併探討二者之間的關繫。方法採用免疫組化EnVision法檢測64例LSCC和15例正常喉黏膜( nor-mal laryngeal mucosa, NLM)組織中KAI1/CD82和CD44v6的錶達。結果 LSCC組織中KAI1/CD82蛋白的暘性率(37．50%)顯著低于NLM組織(86．67%)(P=0．031)；LSCC組織中CD44v6蛋白的暘性率(75．00%)顯著高于NLM組織(26．67%)(P=0．011)。 KAI1/CD82錶達與LSCC臨床分期、分化程度及淋巴結轉移相關(P<0．05),與預後無關(P>0．05)；CD44v6錶達與LSCC分化程度、淋巴結轉移及預後相關(P<0．05),與臨床分期無關(P>0．05)；二者均與患者年齡、性彆無關(P>0．05)。KAI1/CD82與CD44v6在LSCC組織中的錶達呈負相關(rs =-0．504,P=0．036)。結論 KAI1/CD82與CD44v6在LSCC的髮生、髮展和侵襲轉移中起著相互抑製的作用,檢測KAI1/CD82及CD44v6蛋白錶達對判斷LSCC的生物學行為具有重要價值。
목적：탐토종류전이억제기인KAI1/CD82화세포분자CD44v6재인후린상세포암( laryngeal squamous cell carcinoma, LSCC)중적표체급의의,병탐토이자지간적관계。방법채용면역조화EnVision법검측64례LSCC화15례정상후점막( nor-mal laryngeal mucosa, NLM)조직중KAI1/CD82화CD44v6적표체。결과 LSCC조직중KAI1/CD82단백적양성솔(37．50%)현저저우NLM조직(86．67%)(P=0．031)；LSCC조직중CD44v6단백적양성솔(75．00%)현저고우NLM조직(26．67%)(P=0．011)。 KAI1/CD82표체여LSCC림상분기、분화정도급림파결전이상관(P<0．05),여예후무관(P>0．05)；CD44v6표체여LSCC분화정도、림파결전이급예후상관(P<0．05),여림상분기무관(P>0．05)；이자균여환자년령、성별무관(P>0．05)。KAI1/CD82여CD44v6재LSCC조직중적표체정부상관(rs =-0．504,P=0．036)。결론 KAI1/CD82여CD44v6재LSCC적발생、발전화침습전이중기착상호억제적작용,검측KAI1/CD82급CD44v6단백표체대판단LSCC적생물학행위구유중요개치。
Purpose To study the expression of metastasis suppressor gene (KAI1/CD82) and cell adhesion molecules(CD44v6) in human laryngeal squamous cell carcinoma ( LSCC) and its clinical significance, and to investigate their relationship. Methods EnVi-sion immunohistochemistry was used to detect the expression of KAI1/CD82 and CD44v6 protein in 64 cases of LSCC tissues and 15 ca-ses of normal laryngeal mucosa ( NLM) tissues. Results The positive rate of KAI1/CD82 in LSCC tissues ( 37. 50%) was signifi-cantly lower than that of the NLM tissues(86. 67%) (P=0. 031), and the positive rate of CD44v6 in LSCC tissues(75. 00%) was significantly higher than that of the NLM tissues(26. 67%) (P=0. 011). The expression of KAI1/CD82 was associated with clinical stages, grade of tumor differentiation, neck lymph node metastasis ( P<0. 05 ) while not related with age, sex and prognosis ( P>0. 05). And CD44v6 with grade of tumor differentiation, neck lymph node metastasis and prognosis (P<0. 05), but not with age, sex and clinical stages (P>0. 05). In addition, KAI1/CD82 expression was negatively correlated with CD44v6 expression (rs = -0. 504, P=0. 036). Conclusion KAI1/CD82 and CD44v6 are mutually inhibited in the tumorigenesis, progress, invasion and metastasis, and detection of the expression of KAI1/CD82 and CD44v6 may be helpful for judging the biological behaviors of LSCC.
