临床与实验病理学杂志
臨床與實驗病理學雜誌
림상여실험병이학잡지
Chinese Journal of Clinical and Experimental Pathology
2015年
10期
1099-1101,1106
,共4页
食管肿瘤%Cx43%E-cad%免疫组织化学%Western blot
食管腫瘤%Cx43%E-cad%免疫組織化學%Western blot
식관종류%Cx43%E-cad%면역조직화학%Western blot
esophageal neoplasms%Cx43%E-cad%immunohistochemistry%Western blot
目的：观察Cx43和E-cad蛋白在食管鳞癌及正常食管组织中的表达及二者表达的相关性。方法采用免疫组化法及Western blot法检测Cx43和E-cad在食管鳞癌及正常食管组织中的表达,并分析二者表达与临床病理参数之间的关系及二者的相关性。结果免疫组化和Western blot结果显示,Cx43和E-cad在食管鳞癌组织中的表达均显著低于正常食管组织( P<0．05)；Cx43表达与食管鳞癌淋巴结转移有关,E-cad的表达与食管鳞癌呈组织分化程度有关。 Cx43与E-cad在同一例食管鳞癌组织中的表达呈显著正相关。结论 Cx43与E-cad蛋白在食管鳞癌组织中均低表达,联合检测Cx43和E-cad基因表达可能对食管鳞癌的生物学行为和预后评估起一定作用。
目的：觀察Cx43和E-cad蛋白在食管鱗癌及正常食管組織中的錶達及二者錶達的相關性。方法採用免疫組化法及Western blot法檢測Cx43和E-cad在食管鱗癌及正常食管組織中的錶達,併分析二者錶達與臨床病理參數之間的關繫及二者的相關性。結果免疫組化和Western blot結果顯示,Cx43和E-cad在食管鱗癌組織中的錶達均顯著低于正常食管組織( P<0．05)；Cx43錶達與食管鱗癌淋巴結轉移有關,E-cad的錶達與食管鱗癌呈組織分化程度有關。 Cx43與E-cad在同一例食管鱗癌組織中的錶達呈顯著正相關。結論 Cx43與E-cad蛋白在食管鱗癌組織中均低錶達,聯閤檢測Cx43和E-cad基因錶達可能對食管鱗癌的生物學行為和預後評估起一定作用。
목적：관찰Cx43화E-cad단백재식관린암급정상식관조직중적표체급이자표체적상관성。방법채용면역조화법급Western blot법검측Cx43화E-cad재식관린암급정상식관조직중적표체,병분석이자표체여림상병리삼수지간적관계급이자적상관성。결과면역조화화Western blot결과현시,Cx43화E-cad재식관린암조직중적표체균현저저우정상식관조직( P<0．05)；Cx43표체여식관린암림파결전이유관,E-cad적표체여식관린암정조직분화정도유관。 Cx43여E-cad재동일례식관린암조직중적표체정현저정상관。결론 Cx43여E-cad단백재식관린암조직중균저표체,연합검측Cx43화E-cad기인표체가능대식관린암적생물학행위화예후평고기일정작용。
Purpose To investigate the expression of the Cx43 and E-cad protein in esophageal carcinoma and normal esophageal tis-sue and to discuss their relationship. Methods SP immunohistochemical and Western blot analysis were used to detect the expression of Cx43 and E-cad in esophageal carcinoma and normal esophageal tissue. The relation between their expression and esophageal carci-noma was investigated. Results Immunohistochemical assay and Western blot analysis showed the expression levels of Cx43 and E-cad in esophageal carcinoma were significantly lower than that in normal esophageal tissue. The expression of Cx43 had correlation with lymph node metastasis. The expression of E-cad had correlation with degrees of pathological differentiation. There was a positive rela-tionship between the expression of Cx43 and E-cad in same sample. Conclusion Expression of Cx43 and E-cad protein is low in e-sophageal carcinoma, which can be helpful in the prediction of the biological behavior and prognosis of esophageal carcinoma.
