CAJ | 학술논문

目的：探讨重组人促红细胞生成素（rhEPO）对3日龄大鼠缺氧缺血性脑白质损伤的胶质纤维酸性蛋白（GFAP）表达的影响．方法：150只3日龄（P3）SD 大鼠随机分为对照组，脑室周围白质软化（PVL）组和 rEPO 治疗组．于造模后1、7、21 d 对各组大鼠脑质量损伤百分比进行检测，造模后3、7、14、21 d 用免疫组织化学方法观察脑组织 GFAP 表达情况等来研究 EPO 对3日龄大鼠缺血缺氧性脑白质损伤的保护作用．结果：PVL 组和 rEPO 组的脑质量损伤％在各取样时间点均明显大于对照组（P ＜0.05），且 rEPO 组明显小于 PVL 组（P ＜0.05）．HE 染色示 PVL组术后7 d 胼胝体结构疏松，术后21 d 可见侧脑室扩大明显；rEPO 组术后7 d 胼胝体结构基本正常，术后21 d 侧脑室仅稍微扩大．术后3 d PVL 组 GFAP 阳性表达增多（P ＜0.05），术后7 d rEPO 组和 PVL 组 GFAP 阳性表达均明显增多（P ＜0.05），且 PVL 组增多更为明显（P ＜0.05）；术后14 d rEPO 组与 PVL 组 GFAP 阳性表达开始回落，但仍多于对照组（P ＜0.05），PVL 组阳性表达数仍然比 rEPO 组多（P ＜0.05），术后21 d 各组 GFAP 阳性表达数基本回落至正常水平．结论：脑白质损伤时 GFAP 阳性表达增加，rEPO 能减少脑白质损伤后 GFAP 阳性表达．
목적：탐토중조인촉홍세포생성소（rhEPO）대3일령대서결양결혈성뇌백질손상적효질섬유산성단백（GFAP）표체적영향．방법：150지3일령（P3）SD 대서수궤분위대조조，뇌실주위백질연화（PVL）조화 rEPO 치료조．우조모후1、7、21 d 대각조대서뇌질량손상백분비진행검측，조모후3、7、14、21 d 용면역조직화학방법관찰뇌조직 GFAP 표체정황등래연구 EPO 대3일령대서결혈결양성뇌백질손상적보호작용．결과：PVL 조화 rEPO 조적뇌질량손상％재각취양시간점균명현대우대조조（P ＜0.05），차 rEPO 조명현소우 PVL 조（P ＜0.05）．HE 염색시 PVL조술후7 d 변지체결구소송，술후21 d 가견측뇌실확대명현；rEPO 조술후7 d 변지체결구기본정상，술후21 d 측뇌실부초미확대．술후3 d PVL 조 GFAP 양성표체증다（P ＜0.05），술후7 d rEPO 조화 PVL 조 GFAP 양성표체균명현증다（P ＜0.05），차 PVL 조증다경위명현（P ＜0.05）；술후14 d rEPO 조여 PVL 조 GFAP 양성표체개시회락，단잉다우대조조（P ＜0.05），PVL 조양성표체수잉연비 rEPO 조다（P ＜0.05），술후21 d 각조 GFAP 양성표체수기본회락지정상수평．결론：뇌백질손상시 GFAP 양성표체증가，rEPO 능감소뇌백질손상후 GFAP 양성표체．
Aim:To explore the neuroprotection effects of recombinant human erythropoietin(rEPO) on 3 day-old neonatal rats with white matter damage.Methods:150 three day-old Sprague-Dawley(SD) rats were randomly divided into control,periventricular leukomalacia(PVL)and rEPO treatment groups (each group n =50).The brain weight and percentage of the brain damage were recorded at 1 d、7 d and 21 d post operation.Immunohistochemistry method was used to determine the changes of GFAP expres-sion levels in the brain at 3 d、7 d 、14 d and 21 d.Results:The percentage of brain damage in the rEPO and PVL groups was found to be significantly increased compared to the control group(P <0.05)at each sampling time point,but that of rEPO group was less than PVL group(P <0.05);Compared to the con-trol group,HE staining showed that in the rEPO group the structure of callosum was basically normal at 7 d post-operation but the lateral ventricles became mildly expanded at 21 d post-operation.However,a loose callosum structure and dilated lateral ventricles were observed at the same sampling time point in PVL group;The expression of GFAP in PVL group was found to be increased at 3 d post-operation(P <0.05).The expression of GFAP in both rEPO and PVL groups increased significantly compared to that of the control group 7 d after modeling,especially in PVL group(P <0.05).The expression of GFAP in both rEPO and PVL groups increased slightly but still higher than that of the control group(P <0.05),and GFAP expression level in PVL group was higher than that of rEPO group(P <0.05)14 d after modeling. At 21 d post-operation,the expression of GFAP in each group recovered to normal levels.Conclusion:The expression of GFAP increased after damage of the white matter.rEPO treatment can significantly re-duce the expression level of GFAP after the white matter damage.