现代中西医结合杂志
現代中西醫結閤雜誌
현대중서의결합잡지
Modern Journal of Integrated Traditional Chinese and Western Medicine
2015年
30期
3324-3327
,共4页
腹水%CD64%mCD14%肝硬化%自发性细菌性腹膜炎
腹水%CD64%mCD14%肝硬化%自髮性細菌性腹膜炎
복수%CD64%mCD14%간경화%자발성세균성복막염
ascites%CD64%mCD14%hepatocirrhosis%spontaneous bacterial peritonitis
目的：探讨肝硬化合并自发性细菌性腹膜炎（ SBP）患者腹水CD64及外周血mCD14的变化及其意义。方法选取肝硬化合并腹水患者87例，根据是否合并SBP将患者分为2组：合并SBP 44例为研究组，未合并SBP 43例为对照组，采用流式细胞术检测2组患者腹水中性粒细胞和淋巴细胞CD64平均荧光强度、CD64指数，外周血白细胞计数、mCD14阳性表达率、mCD14平均荧光强度和mCD14指数。研究组抗感染治疗1周后再按治疗有效与无效、生存与死亡分组评价腹水中性粒细胞和淋巴细胞CD64平均荧光强度、CD64指数，外周血mCD14平均荧光强度和mCD14指数。结果研究组腹水中性粒细胞CD64平均荧光强度、CD64指数和外周血白细胞计数明显高于对照组（P均＜0．05），淋巴细胞CD64平均荧光强度、mCD14阳性表达率、mCD14平均荧光强度和mCD14指数均明显低于对照组（ P均＜0．05）。治疗有效组和生存组中性粒细胞CD64平均荧光强度和CD64指数均明显低于治疗无效组和死亡组（P均＜0．05），淋巴细胞CD64平均荧光强度、mCD14平均荧光强度和mCD14指数均明显高于治疗无效组和死亡组（P均＜0．05）。腹水CD64与外周血mCD14联合检测对治疗效果和预后判断的ROC曲线下面积、临界值、灵敏度和特异度均明显高于CD64、mCD14单项检测（P均＜0．05）。结论肝硬化合并SBP患者腹水中性粒细胞CD64显著增高，腹水淋巴细胞CD64与外周血mCD14显著降低，腹水CD64与外周血mCD14联合检测预示肝硬化合并SBP治疗效果和预后的价值显著。
目的：探討肝硬化閤併自髮性細菌性腹膜炎（ SBP）患者腹水CD64及外週血mCD14的變化及其意義。方法選取肝硬化閤併腹水患者87例，根據是否閤併SBP將患者分為2組：閤併SBP 44例為研究組，未閤併SBP 43例為對照組，採用流式細胞術檢測2組患者腹水中性粒細胞和淋巴細胞CD64平均熒光彊度、CD64指數，外週血白細胞計數、mCD14暘性錶達率、mCD14平均熒光彊度和mCD14指數。研究組抗感染治療1週後再按治療有效與無效、生存與死亡分組評價腹水中性粒細胞和淋巴細胞CD64平均熒光彊度、CD64指數，外週血mCD14平均熒光彊度和mCD14指數。結果研究組腹水中性粒細胞CD64平均熒光彊度、CD64指數和外週血白細胞計數明顯高于對照組（P均＜0．05），淋巴細胞CD64平均熒光彊度、mCD14暘性錶達率、mCD14平均熒光彊度和mCD14指數均明顯低于對照組（ P均＜0．05）。治療有效組和生存組中性粒細胞CD64平均熒光彊度和CD64指數均明顯低于治療無效組和死亡組（P均＜0．05），淋巴細胞CD64平均熒光彊度、mCD14平均熒光彊度和mCD14指數均明顯高于治療無效組和死亡組（P均＜0．05）。腹水CD64與外週血mCD14聯閤檢測對治療效果和預後判斷的ROC麯線下麵積、臨界值、靈敏度和特異度均明顯高于CD64、mCD14單項檢測（P均＜0．05）。結論肝硬化閤併SBP患者腹水中性粒細胞CD64顯著增高，腹水淋巴細胞CD64與外週血mCD14顯著降低，腹水CD64與外週血mCD14聯閤檢測預示肝硬化閤併SBP治療效果和預後的價值顯著。
목적：탐토간경화합병자발성세균성복막염（ SBP）환자복수CD64급외주혈mCD14적변화급기의의。방법선취간경화합병복수환자87례，근거시부합병SBP장환자분위2조：합병SBP 44례위연구조，미합병SBP 43례위대조조，채용류식세포술검측2조환자복수중성립세포화림파세포CD64평균형광강도、CD64지수，외주혈백세포계수、mCD14양성표체솔、mCD14평균형광강도화mCD14지수。연구조항감염치료1주후재안치료유효여무효、생존여사망분조평개복수중성립세포화림파세포CD64평균형광강도、CD64지수，외주혈mCD14평균형광강도화mCD14지수。결과연구조복수중성립세포CD64평균형광강도、CD64지수화외주혈백세포계수명현고우대조조（P균＜0．05），림파세포CD64평균형광강도、mCD14양성표체솔、mCD14평균형광강도화mCD14지수균명현저우대조조（ P균＜0．05）。치료유효조화생존조중성립세포CD64평균형광강도화CD64지수균명현저우치료무효조화사망조（P균＜0．05），림파세포CD64평균형광강도、mCD14평균형광강도화mCD14지수균명현고우치료무효조화사망조（P균＜0．05）。복수CD64여외주혈mCD14연합검측대치료효과화예후판단적ROC곡선하면적、림계치、령민도화특이도균명현고우CD64、mCD14단항검측（P균＜0．05）。결론간경화합병SBP환자복수중성립세포CD64현저증고，복수림파세포CD64여외주혈mCD14현저강저，복수CD64여외주혈mCD14연합검측예시간경화합병SBP치료효과화예후적개치현저。
ObjectiveIt is to investigate the change and significance of ascites CD 64 and peripheral mCD14for hepatocir-rhosis patients with spontaneous bacterial peritonitis (SBP).Methods 87 hepatocirrhosis patients with ascites were selected and divided into two groups according to whether combined SBP, 44 cases combined SBP in research group, and 43 cases none SBP in control group.Flow cytometry was used to detect the average fluorescent intensity and index of CD 64 in ascites neutro-phils and lymphocytes, and the peripheral blood white blood cell count , mCD14 positive expression rate, mCD14 average fluo-rescence intensity and mCD14 index of the two groups.The fluorescent intensity and index of CD64 in ascites neutrophils and lymphoctesand the peripheral blood mCD14 in research group were compared according to effective and invalid, survival and death respectively.Results The neutrophils CD64 average fluorescence intensity and CD64 index of and peripheral blood leu-kocyte count of the research group were significantly higher than those of the control group (all P<0.05), the lymphocyte CD64 average fluorescence intensity, mCD14 positive expression rate, monocytes mCD14 average fluorescence intensity and mCD14 index were significantly lower than those of the control group (all P<0.05).The neutrophils CD64 average fluores-cence intensity and CD64 index of effective treatment/survival group were significantly lower than those of ineffective /death group(all P<0.05), and lymphocyte CD64 average fluorescence intensity, monocytes mCD14 average fluorescence intensity and mCD14 index were significantly higher than those of ineffective /death group ( all P<0.05 ) .The area under the ROC curve ( AUC) , the critical value, sensitivity and speciality rate of ascites CD64 and peripheral blood mCD14 combined detec-tion for therapeutic effect and prognosis prediction were significantly higher than those of CD64 or mCD14 single detection ( all P<0.05) .Conclusion Ascites neutrophils CD64 level is significantly higher, ascites lymphocytes CD64 and peripheral blood mononuclear cells mCD14 levels is decreased significantly, the combined detection of ascites CD64 and peripheral blood mCD14 has significant value in the predicts therapeutic effect and prognosis for hepatocirrhosis with SBP.
