CAJ | 학술논문

目的：通过测定膝骨关节炎兔模型血清和关节液中TNF?α、IL?1β的含量，观察软骨的组织学改变，探讨小白菊内酯不同给药途径治疗骨关节炎的疗效与作用机制。方法健康纯种新西兰兔40只，随机取8只作为正常组（ A组）；其余的32只采用右后肢伸直位管型石膏固定法，建立OA模型。造模后随机分为4组：模型口服对照组（ B组）、模型关节腔注射对照组（C）、小白菊内酯口服组（D组）、小白菊内酯关节腔注射组（E组），每组8只。 B组和D组兔分别予生理盐水、小白菊内酯灌胃，C组和E组兔分别予生理盐水、小白菊内酯关节腔注射，连续治疗6w后，测定各组兔治疗前后血清、膝关节液TNF?α、IL?1β表达水平。所有实验动物在8w处死，应用苏木素?伊红（HE）染色观察各组动物关节软骨的病理组织改变。结果与模型组（ B组、C组）比较，D组、E组关节液及血清中的TNF?α、IL?1β浓度均明显降低（ P＜0?05），且E组降低幅度明显大于D组（P＜0?05）。与A组比较，B组和C组病理显示有关节软骨破坏，病理积分升高（P＜0?05）；D组、E组病理改变较B组、C组显示关节软骨有所修复，病理积分降低（P＜0?05）。结论小白菊内酯可减轻动物模型的关节炎症，其关节腔给药的抗炎疗效优于口服给药，抑制血清和关节腔TNF?α、IL?1β的分泌，可能是小白菊内酯治疗骨关节炎的机制之一。
목적：통과측정슬골관절염토모형혈청화관절액중TNF?α、IL?1β적함량，관찰연골적조직학개변，탐토소백국내지불동급약도경치료골관절염적료효여작용궤제。방법건강순충신서란토40지，수궤취8지작위정상조（ A조）；기여적32지채용우후지신직위관형석고고정법，건립OA모형。조모후수궤분위4조：모형구복대조조（ B조）、모형관절강주사대조조（C）、소백국내지구복조（D조）、소백국내지관절강주사조（E조），매조8지。 B조화D조토분별여생리염수、소백국내지관위，C조화E조토분별여생리염수、소백국내지관절강주사，련속치료6w후，측정각조토치료전후혈청、슬관절액TNF?α、IL?1β표체수평。소유실험동물재8w처사，응용소목소?이홍（HE）염색관찰각조동물관절연골적병리조직개변。결과여모형조（ B조、C조）비교，D조、E조관절액급혈청중적TNF?α、IL?1β농도균명현강저（ P＜0?05），차E조강저폭도명현대우D조（P＜0?05）。여A조비교，B조화C조병리현시유관절연골파배，병리적분승고（P＜0?05）；D조、E조병리개변교B조、C조현시관절연골유소수복，병리적분강저（P＜0?05）。결론소백국내지가감경동물모형적관절염증，기관절강급약적항염료효우우구복급약，억제혈청화관절강TNF?α、IL?1β적분비，가능시소백국내지치료골관절염적궤제지일。
Objective By measuring the levels of TNF?α and IL?1β in serum and synovial fluid in rabbit model of knee osteoarthritis ( OA) , to observe the histological changes of the cartilage and to explore the efficacy and mechanism of parthenolide on osteoarthritis with different administration routes. Methods Forty healthy purebred New Zealand rabbits were randomly divided into control group (group A, n=8), and OA model groups established using plaster cast immobilization of the right hind limb extension (n=32). After modeling the rabbits were randomly divided into 4 groups, the model of oral control group (group B), model of intra?articular injection control group (group C), parthenolide oral group (group D), parthenolide articular injection group (group E) , with 8 rabbits in each group. After 6?week treatment, the expression levels of TNF?αand IL?1βin the serum and knee fluid were measured. All animals were sacrificed at 8 weeks, the histopathological changes in articular cartilage were observed using hematoxylin?eosin ( HE) staining. Results TNF?α and IL?1β concentrations in synovial fluid and serum were significantly lower in rabbits of group D and E than those in group B and C ( P<0?05 ) , and the decrease was more significant in group E than in group D ( P<0?05). Comparing with those in group A, the relevant sections in group B and C showed pathological cartilage damage, and the pathological scores increased (P<0?05). Comparing with those in group D and E, the pathological changes in group B and C showed some repair in articular cartilage, and the pathological scores decreased (P<0?05). Conclusion Parthenolide relieves arthritis in the animal model. The anti?inflammatory effect of intra?articular administration is better than oral administration. It inhibits secretion of serum and articular TNF?αand IL?1β, which may be one of the mechanisms of parthenolide in the treatment of arthritis.