中国骨质疏松杂志
中國骨質疏鬆雜誌
중국골질소송잡지
Chinese Journal of Osteoporosis
2015年
9期
1029-1034
,共6页
于琼%吕思敏%崔燎%吴铁%王勤
于瓊%呂思敏%崔燎%吳鐵%王勤
우경%려사민%최료%오철%왕근
环磷酰胺%大鼠%骨质疏松%Micro-CT%骨生物力学
環燐酰胺%大鼠%骨質疏鬆%Micro-CT%骨生物力學
배린선알%대서%골질소송%Micro-CT%골생물역학
Cyclophosphamide%Rats%Osteoporosis%Micro-CT%Biomechanical property
目的:应用骨生物力学测试和micro-CT技术,观察环磷酰胺诱导大鼠骨质疏松的时效关系。方法3月龄SPF级雄性SD大鼠用环磷酰胺4.5 mg?kg-1?d-1连续灌胃15 d,停药后的0、15和30 d,取右侧股骨进行生物力学检测,然后进行Micro-CT扫描及三维重建。结果大鼠环磷酰胺诱导15 d后,股骨松质骨的三维结构和评价参数均呈现明显的微观结构退化,小梁骨结构由板状向杆状变化,而生物力学性能降低,骨脆性增加,骨折风险增加。停药后15d和30d,股骨松质骨的三维结构和评价参数仍然呈现明显的骨质疏松变化,除韧性系数和刚性系数外,其他生物力学参数仍然低下。停药后,环磷酰胺组大鼠以上各骨评价参数虽呈现了稳定的增长趋势,但较正常大鼠明显减弱。结论环磷酰胺诱导的大鼠骨质疏松模型近期效果好,停药后1个月内,骨增长的抑制效果虽有所减弱,但骨丢失仍然明显。
目的:應用骨生物力學測試和micro-CT技術,觀察環燐酰胺誘導大鼠骨質疏鬆的時效關繫。方法3月齡SPF級雄性SD大鼠用環燐酰胺4.5 mg?kg-1?d-1連續灌胃15 d,停藥後的0、15和30 d,取右側股骨進行生物力學檢測,然後進行Micro-CT掃描及三維重建。結果大鼠環燐酰胺誘導15 d後,股骨鬆質骨的三維結構和評價參數均呈現明顯的微觀結構退化,小樑骨結構由闆狀嚮桿狀變化,而生物力學性能降低,骨脆性增加,骨摺風險增加。停藥後15d和30d,股骨鬆質骨的三維結構和評價參數仍然呈現明顯的骨質疏鬆變化,除韌性繫數和剛性繫數外,其他生物力學參數仍然低下。停藥後,環燐酰胺組大鼠以上各骨評價參數雖呈現瞭穩定的增長趨勢,但較正常大鼠明顯減弱。結論環燐酰胺誘導的大鼠骨質疏鬆模型近期效果好,停藥後1箇月內,骨增長的抑製效果雖有所減弱,但骨丟失仍然明顯。
목적:응용골생물역학측시화micro-CT기술,관찰배린선알유도대서골질소송적시효관계。방법3월령SPF급웅성SD대서용배린선알4.5 mg?kg-1?d-1련속관위15 d,정약후적0、15화30 d,취우측고골진행생물역학검측,연후진행Micro-CT소묘급삼유중건。결과대서배린선알유도15 d후,고골송질골적삼유결구화평개삼수균정현명현적미관결구퇴화,소량골결구유판상향간상변화,이생물역학성능강저,골취성증가,골절풍험증가。정약후15d화30d,고골송질골적삼유결구화평개삼수잉연정현명현적골질소송변화,제인성계수화강성계수외,기타생물역학삼수잉연저하。정약후,배린선알조대서이상각골평개삼수수정현료은정적증장추세,단교정상대서명현감약。결론배린선알유도적대서골질소송모형근기효과호,정약후1개월내,골증장적억제효과수유소감약,단골주실잉연명현。
Objective To investigate the time-effect relationship in cyclophosphamide-induced osteoporosis in rats using micro-CT and biomechanical test.Methods Three-month-old male Sprague-Dawley rats were treated with cyclophosphamide (4.5 mg?kg-1?d-1 ) orally once a day for successive 15 days.After drug withdrawal for 0, 15, and 30d, micro-CT and biomechanical test were performed and the 3D reconstruction was analyzed in the right femur.Results After 15d cyclophosphamide induction, the three-dimensional structure and the evaluation parameters of the cancellous bone in the femur presented obvious microstructure degradation in rats.The biomechanical performance of the femur reduced, while the bone fragility and fracture risks increased.After drug withdrawal for 15d or 30d, the three-dimensional structure and the evaluation parameters of cancellous bone in the femur still displayed apparent osteoporotic feature.Except for the rigidity modulus and toughness index, other biomechanical parameters were similar to those in the cyclophosphamide-induced rats.After drug withdrawal, assessment parameters of the cancellous bone and mechanical property of the rat femur showed a stable increase in the cyclophosphamide-induced rats.However, the increase trend was obviously weaker than that in normal rats.Conclusion The cyclophosphamide-induced osteoporosis rat model shows good performance in short-term investigation.Inhibition of bone growth weakens after 1-month drug withdrawal but bone loss is still obvious.