CAJ | 학술논문

目的：分析OPG基因的单核苷酸多态性（ SNP）与绝经前后妇女骨密度的关系。方法在235名绝经前后妇女中，采用基质辅助激光解吸电离飞行时间质谱技术对6个护骨素（ OPG）基因的SNP进行分型。应用双能X线骨密度仪测定腰椎、髋部和股骨颈骨密度（BMD）。结果 rs6993813TT基因型股骨颈BMD显著高于CC型，CC型各部位的BMD均低于TC／TT型（P＜0．05）。 rs4355801GG型股骨颈的BMD高于AG／AA型（P＜0．05）。 rs1032129 AA型股骨颈的BMD高于AC／CC型（P＜0．05）。 rs2073618的CC基因型股骨颈的BMD显著高于GG／GC型（P＜0．05）。结论 rs6993813， rs4355801， rs1032129和rs2073618均与中国绝经前后女性BMD有关，其危险基因分别为C， A， C和G，而T，G，A和C则具有保护作用，含有保护基因者骨密度高于含有危险基因者，但确切的机制尚需在今后工作中进一步研究阐明。
목적：분석OPG기인적단핵감산다태성（ SNP）여절경전후부녀골밀도적관계。방법재235명절경전후부녀중，채용기질보조격광해흡전리비행시간질보기술대6개호골소（ OPG）기인적SNP진행분형。응용쌍능X선골밀도의측정요추、관부화고골경골밀도（BMD）。결과 rs6993813TT기인형고골경BMD현저고우CC형，CC형각부위적BMD균저우TC／TT형（P＜0．05）。 rs4355801GG형고골경적BMD고우AG／AA형（P＜0．05）。 rs1032129 AA형고골경적BMD고우AC／CC형（P＜0．05）。 rs2073618적CC기인형고골경적BMD현저고우GG／GC형（P＜0．05）。결론 rs6993813， rs4355801， rs1032129화rs2073618균여중국절경전후녀성BMD유관，기위험기인분별위C， A， C화G，이T，G，A화C칙구유보호작용，함유보호기인자골밀도고우함유위험기인자，단학절적궤제상수재금후공작중진일보연구천명。
Objective To explore the relationship between single nucleotide polymorphism ( SNP) of osteoprotegerin ( OPG) and bone mineral density ( BMD) in pre-and post-menopausal women.Methods A cross-sectional study was conducted in 108 premenopausal and 127 postmenopausal women.Six genotypes of OPG were determined using chip-based matrix-assisted laser desorption ionization time-of-flight mass spectrometry.BMD of the lumbar spine, hip, and the femoral neck was evaluated using dual energy X-ray absorptiometry.Results BMD of the femoral neck in SNP rs6993813TT patients was significantly higher than that in TC/CC type patients (P<0.05).BMD of all parts in CC type patients was lower than that in TC/TT patients.BMD of the femoral neck in SNP rs4355801GG patients was significantly higher than that in AG/AA type patients (P<0.05).BMD of the femoral neck in SNP rs1032129AA patients was significantly higher than that in AC/CC type patients (P<0.05).BMD of the femoral neck in SNP rs2073618CC patients was significantly higher than that in GG/GC type patients (P<0.05).Conclusion The observed risk alleles of rs6993813, rs4355801, rs1032129, and rs2073618 in the present study were C, A, C, and G, respectively.By contrast, the protective alleles were T, G, A, and C, respectively.Women with protective alleles had higher BMD than women with risk alleles.The detailed mechanism needs further work to clarify.