现代医药卫生
現代醫藥衛生
현대의약위생
Journal of Modern Medicine & Health
2015年
20期
3056-3058,3061
,共4页
阳又莲%张新%罗扬%刘宿
暘又蓮%張新%囉颺%劉宿
양우련%장신%라양%류숙
姜黄素%脑损伤%炎症%巨噬细胞%小神经胶质细胞%免疫组织化学
薑黃素%腦損傷%炎癥%巨噬細胞%小神經膠質細胞%免疫組織化學
강황소%뇌손상%염증%거서세포%소신경효질세포%면역조직화학
Curcumin%Brain injury%Inflammation%Macrophage%Microglia%Immunohistochemistry
目的：探究姜黄素对创伤性脑损伤（TBI）的治疗作用及机制。方法将12只SD大鼠随机分为姜黄素组（6只）和DMSO组（6只），为每只大鼠建立TBI模型，治疗组给予姜黄素治疗3 d，对照组腹腔注射等量DMSO。每天测量体质量和神经功能评分至实验结束。在第5天（高峰期）取大鼠脑组织，用免疫组织化学法染色观察巨噬细胞/小胶质细胞炎症浸润，用实时定量聚合酶链反应（RT-PCR）方法检测炎症因子肿瘤坏死因子-α（TNF-α）、白介素（IL）-1β的相对表达。第25天（恢复期）取大鼠脑组织，用RT-PCR方法检测IL-10、转化生长因子β（TGF-β）的相对表达。结果神经功能评分显示姜黄素治疗后大鼠TBI严重度显著降低，姜黄素能抑制TBI高峰期巨噬细胞在大鼠脑组织中的炎症浸润与小胶质细胞的激活，抑制TBI高峰期脑组织TNF-α、IL-1β表达，促进TBI恢复期IL-10及TGF-β的表达。结论姜黄素对TBI有治疗作用，这种治疗作用可能与姜黄素抑制TBI后炎性反应、促进组织恢复有关。
目的：探究薑黃素對創傷性腦損傷（TBI）的治療作用及機製。方法將12隻SD大鼠隨機分為薑黃素組（6隻）和DMSO組（6隻），為每隻大鼠建立TBI模型，治療組給予薑黃素治療3 d，對照組腹腔註射等量DMSO。每天測量體質量和神經功能評分至實驗結束。在第5天（高峰期）取大鼠腦組織，用免疫組織化學法染色觀察巨噬細胞/小膠質細胞炎癥浸潤，用實時定量聚閤酶鏈反應（RT-PCR）方法檢測炎癥因子腫瘤壞死因子-α（TNF-α）、白介素（IL）-1β的相對錶達。第25天（恢複期）取大鼠腦組織，用RT-PCR方法檢測IL-10、轉化生長因子β（TGF-β）的相對錶達。結果神經功能評分顯示薑黃素治療後大鼠TBI嚴重度顯著降低，薑黃素能抑製TBI高峰期巨噬細胞在大鼠腦組織中的炎癥浸潤與小膠質細胞的激活，抑製TBI高峰期腦組織TNF-α、IL-1β錶達，促進TBI恢複期IL-10及TGF-β的錶達。結論薑黃素對TBI有治療作用，這種治療作用可能與薑黃素抑製TBI後炎性反應、促進組織恢複有關。
목적：탐구강황소대창상성뇌손상（TBI）적치료작용급궤제。방법장12지SD대서수궤분위강황소조（6지）화DMSO조（6지），위매지대서건립TBI모형，치료조급여강황소치료3 d，대조조복강주사등량DMSO。매천측량체질량화신경공능평분지실험결속。재제5천（고봉기）취대서뇌조직，용면역조직화학법염색관찰거서세포/소효질세포염증침윤，용실시정량취합매련반응（RT-PCR）방법검측염증인자종류배사인자-α（TNF-α）、백개소（IL）-1β적상대표체。제25천（회복기）취대서뇌조직，용RT-PCR방법검측IL-10、전화생장인자β（TGF-β）적상대표체。결과신경공능평분현시강황소치료후대서TBI엄중도현저강저，강황소능억제TBI고봉기거서세포재대서뇌조직중적염증침윤여소효질세포적격활，억제TBI고봉기뇌조직TNF-α、IL-1β표체，촉진TBI회복기IL-10급TGF-β적표체。결론강황소대TBI유치료작용，저충치료작용가능여강황소억제TBI후염성반응、촉진조직회복유관。
Objective To investigate the therapeutic effect and the potential mechanism of curcumin for treating trau-matic brain injury(TBI). Methods 12 SD rats were randomly divided into the curcumin group(6 cases) and the DMSO group (control,6 cases). After TBI model was established,each rat in the curcumin group was treated with curcumin for 3 d,while the DMSO group was given the equal quantity of DMSO by intraperitoneal injection. The body mass and neurological functional score were daily measured from the beginning to the end of experiment. On 5 d(peak period),the brain tissue was taken for observing the macrophage/microglia inflammatory infiltration in the brain by the immunohistochemistry staining. The real time quantitative RT-PCR was performed for detecting the relative expression of TNF-αand IL-1β. On 25 d(recovery period),the brain tissue was taken for detecting the relative expressions of IL-10 and TGF-βin brain by RT-PCR. Results The neurological functional scores showed that the severity degrees of the rat TBI were significantly decreased after curcumin treatment ,indicating that curcumin could inhibit the macrophage infiltration and microglia activation in the rat brain tissue ,inhibited the expression of TNF-αand IL-1βin the brain tissue during the peak period and promoted the expression of IL-10 and TGF-βduring the recovery period of TBI. Conclusion Curcumin has the curative effect on TBI,which may be related with curcumin inhibiting the inflammatory reaction after TBI and promoting the tissue recovery.
