CAJ | 학술논문

目的：探讨骨化三醇[1，25（OH）2 D 3]对支气管哮喘患者外周血Th17/Treg表达的调节作用。方法收集55例支气管哮喘患者，其中急性发作组20例、轻度缓解组20例、中重度缓解组15例，同时设正常对照组15例，酶联免疫分析法（ELISA法）检测血浆中1，25（OH）2D3的含量，流式细胞分析技术检测Th17/CD4+（%）、Treg/CD4+（%）。结果哮喘各组血浆中1，25（OH）2D3含量均低于正常对照组，差异有统计学意义（P＜0.05）；哮喘中重度缓解组最低，与其他三组比较差异均有统计学意义（P＜0.05）。1，25（OH）2D3干预前哮喘各组与正常对照组比较Th17/CD4+（%）增高，Treg/CD4+（%）降低，以急性发作组及中重度缓解组为著，差异有统计学意义（P＜0.05）；1，25（OH）2D3干预后哮喘各组Th17/CD4+（%）较前降低，Treg/CD4+（%）较前升高，以急性发作组及中重度缓解组为著，与轻度缓解组比较差异有统计学意义（P＜0.05）。结论哮喘患者外周血中可能存在一定程度维生素D缺乏现象，以中重度缓解组为著。哮喘患者外周血存在Th17/Treg表达失衡，1，25（OH）2D3参与哮喘患者Th17/Treg表达的调控，并在一定程度上改善哮喘患者Th17/Treg失衡状态。
목적：탐토골화삼순[1，25（OH）2 D 3]대지기관효천환자외주혈Th17/Treg표체적조절작용。방법수집55례지기관효천환자，기중급성발작조20례、경도완해조20례、중중도완해조15례，동시설정상대조조15례，매련면역분석법（ELISA법）검측혈장중1，25（OH）2D3적함량，류식세포분석기술검측Th17/CD4+（%）、Treg/CD4+（%）。결과효천각조혈장중1，25（OH）2D3함량균저우정상대조조，차이유통계학의의（P＜0.05）；효천중중도완해조최저，여기타삼조비교차이균유통계학의의（P＜0.05）。1，25（OH）2D3간예전효천각조여정상대조조비교Th17/CD4+（%）증고，Treg/CD4+（%）강저，이급성발작조급중중도완해조위저，차이유통계학의의（P＜0.05）；1，25（OH）2D3간예후효천각조Th17/CD4+（%）교전강저，Treg/CD4+（%）교전승고，이급성발작조급중중도완해조위저，여경도완해조비교차이유통계학의의（P＜0.05）。결론효천환자외주혈중가능존재일정정도유생소D결핍현상，이중중도완해조위저。효천환자외주혈존재Th17/Treg표체실형，1，25（OH）2D3삼여효천환자Th17/Treg표체적조공，병재일정정도상개선효천환자Th17/Treg실형상태。
Objective To detectin vitro study in the regulation of peripheral blood Th17/Treg in bronchial asthma by calcitriolon.Methods55 patients with asthma were recruited and divided into three groups, the acute exacerbation group of 20 cases, the mild remission group of 20 cases and the moderate to severe remission group of 15 cases. At the same time, the normal group of 15 cases were set up as contrast. The content of the 1,25-(OH)2D3 in the plasma was detected by ELISA. The Th17/CD4+(%) and the Treg/CD4+(%) were detected by flow cytometry.ResultsCompared with the normal group, the asthmatic groups had lower content of the 1,25-(OH)2D3in the plasma and the difference was statistically significant (P<0.05). The content of the 1,25-(OH)2D3 in the plasma of the moderate to severe remission group was significantly lower than that of the other three groups (P<0.05). Before the intervention of 1, 25-(OH)2D3, compared with the normal group, the asthmatic groups had a higher Th17/CD4+(%) and a lower Treg/CD4+(%), especially the acute exacerbation group and the moderate to severe remission group, which was statistically significant (P<0.05). After the intervention of 1, 25-(OH)2D3, the Th17/CD4+(%) was lower than before and Treg/CD4+(%) was higher than before in the asthmatic groups, especially the acute exacerbation group and the moderate to severe remission group, compared with the mild remission group, the difference was statistically significant (P<0.05). ConclusionIn vitro, to same extent, the bronchial asthma of peripheral blood were lack of vitamin D3, especially the moderate to severe remission group. The imbalanced expression of Th17/Treg was existed in bronchial asthma of peripheral blood, 125-(OH)2D3 involves in the regulation of Th17/Treg and it improves the imbalance of Th17/Treg to a certain extent.