暨南大学学报（自然科学与医学版）
暨南大學學報（自然科學與醫學版）
기남대학학보（자연과학여의학판）
Journal of Jinan University (Natural Science & Medicine Edition)
2015年
5期
402-405
,共4页
自发性高血压大鼠%脂联素%左室舒张功能%超声心动描记术
自髮性高血壓大鼠%脂聯素%左室舒張功能%超聲心動描記術
자발성고혈압대서%지련소%좌실서장공능%초성심동묘기술
spontaneously hypertensive rat%adiponectin%left ventricular diastolic function%echocardiography
目的：探讨自发性高血压大鼠（SHR）血清脂联素水平与左室舒张功能的关系．方法：6月龄雄性 SHR和 Wistar 大鼠各10只．采用酶联免疫吸附方法测定血清脂联素水平，小动物高频超声检测仪通过二尖瓣血流频谱多普勒和组织多普勒技术测定左室舒张功能各项指标．结果：血清脂联素水平 SHR 显著低于 Wistar 大鼠[（11.18±0.37）、（13.43±0.58）；P ＜0.01]；E ／A 和 E′／A′SHR 显著低于 Wistar 大鼠[E ／A：（2.21±0.23）、（2.77±0.24）；P ＜0.01和 E′／A′：（0.56±0.13）、（1.46±0.11）；P ＜0.01]；E ／E′SHR 显著高于 Wistar 大鼠[（30.17±1.78）、（21.15±1.44）；P ＜0.01]；DT 和 IVRT 两组比较无统计学差异．SHR 血清脂联素水平与 E ／A、E′／A′呈显著正相关（r ＝0.828，P ＝0.003；r ＝0.720，P ＝0.019），与 E ／E′呈显著负相关（r ＝－0.845，P ＝0.002）．多元逐步回归分析表明血清脂联素水平能分别进入以 E ／A、E′／A′和 E ／E′为应变量的回归方程中．结论：血清脂联素水平与左室舒张功能超声指标显著相关，其水平降低可能是导致高血压左室舒张功能不全的危险因素之一．
目的：探討自髮性高血壓大鼠（SHR）血清脂聯素水平與左室舒張功能的關繫．方法：6月齡雄性 SHR和 Wistar 大鼠各10隻．採用酶聯免疫吸附方法測定血清脂聯素水平，小動物高頻超聲檢測儀通過二尖瓣血流頻譜多普勒和組織多普勒技術測定左室舒張功能各項指標．結果：血清脂聯素水平 SHR 顯著低于 Wistar 大鼠[（11.18±0.37）、（13.43±0.58）；P ＜0.01]；E ／A 和 E′／A′SHR 顯著低于 Wistar 大鼠[E ／A：（2.21±0.23）、（2.77±0.24）；P ＜0.01和 E′／A′：（0.56±0.13）、（1.46±0.11）；P ＜0.01]；E ／E′SHR 顯著高于 Wistar 大鼠[（30.17±1.78）、（21.15±1.44）；P ＜0.01]；DT 和 IVRT 兩組比較無統計學差異．SHR 血清脂聯素水平與 E ／A、E′／A′呈顯著正相關（r ＝0.828，P ＝0.003；r ＝0.720，P ＝0.019），與 E ／E′呈顯著負相關（r ＝－0.845，P ＝0.002）．多元逐步迴歸分析錶明血清脂聯素水平能分彆進入以 E ／A、E′／A′和 E ／E′為應變量的迴歸方程中．結論：血清脂聯素水平與左室舒張功能超聲指標顯著相關，其水平降低可能是導緻高血壓左室舒張功能不全的危險因素之一．
목적：탐토자발성고혈압대서（SHR）혈청지련소수평여좌실서장공능적관계．방법：6월령웅성 SHR화 Wistar 대서각10지．채용매련면역흡부방법측정혈청지련소수평，소동물고빈초성검측의통과이첨판혈류빈보다보륵화조직다보륵기술측정좌실서장공능각항지표．결과：혈청지련소수평 SHR 현저저우 Wistar 대서[（11.18±0.37）、（13.43±0.58）；P ＜0.01]；E ／A 화 E′／A′SHR 현저저우 Wistar 대서[E ／A：（2.21±0.23）、（2.77±0.24）；P ＜0.01화 E′／A′：（0.56±0.13）、（1.46±0.11）；P ＜0.01]；E ／E′SHR 현저고우 Wistar 대서[（30.17±1.78）、（21.15±1.44）；P ＜0.01]；DT 화 IVRT 량조비교무통계학차이．SHR 혈청지련소수평여 E ／A、E′／A′정현저정상관（r ＝0.828，P ＝0.003；r ＝0.720，P ＝0.019），여 E ／E′정현저부상관（r ＝－0.845，P ＝0.002）．다원축보회귀분석표명혈청지련소수평능분별진입이 E ／A、E′／A′화 E ／E′위응변량적회귀방정중．결론：혈청지련소수평여좌실서장공능초성지표현저상관，기수평강저가능시도치고혈압좌실서장공능불전적위험인소지일．
Aim:To investigate the relationship of serum adiponectin with echocardiographic indexes of left ventricular diastolic function in spontaneously hypertensive rats (SHR).Methods:Ten male SHR and normotensive Wistar controls were used respectively.Serum adiponectin level was determined by en-zyme-linked immunosorbnent assay (ELISA).Left ventricular diastolic function parameters[E /A,E′/A′,E /E′,deceleration time (DT)and isovolumic relaxation time (IVRT)]were measured and calcu-lated by Pulsed-wave and Tissue Doppler echocardiography.Results:Serum adiponectin level was signif-icantly lower in SHR than that in Wistar rats [(11.18 ±0.37)、(13.43 ±0.58);P <0.01 ],so were E /A and E′/A′ratios [E /A:(2.21 ±0.23)、(2.77 ±0.24);P <0.01 and E′/A′:(0.56 ±0.13)、(1.46 ±0.11);P <0.01].While E /E′ratio was significantly higher in SHR compared with Wistar rats [(30.17 ±1.78)、(21.15 ±1.44);P <0.01].There were no significant differences pertaining to DT and IVRT.In SHR,serum adiponectin level significantly positively correlated with E /A and E′/A′(r =0.828,P =0.003 and r =0.720,P =0.019,respectively)while negatively correlated with E /E′ratio (r =-0.845,P =0.002).Through multiple stepwise regression analysis,adiponectin level could be entered the regression formula for E /A,E′/A′and E /E′,respectively.Conclusion:The present study demonstrated that serum adiponectin level significantly correlated with echocardiographic indexes of left ventricular diastolic function and that it is an independent risk factor for compromised left ventricular di-astolic function.
