广西医学
廣西醫學
엄서의학
Guangxi Medical Journal
2015年
8期
1069-1071
,共3页
钟漓%张广钰%董陈诚%王振冉
鐘巑%張廣鈺%董陳誠%王振冉
종리%장엄옥%동진성%왕진염
胃癌%塞来昔布%凋亡%BAX基因%Bcl-2基因
胃癌%塞來昔佈%凋亡%BAX基因%Bcl-2基因
위암%새래석포%조망%BAX기인%Bcl-2기인
Gastric cancer%Celecoxib%Apoptosis%BAX gene%Bcl-2 gene
目的 观察塞来昔布对人胃癌细胞SGC-7901凋亡的影响并探讨其机制. 方法 将人胃癌细胞SGC-7901细胞分为4组,分别加入不同浓度塞来昔布,低剂量组(25 μm)、中剂量组(50 μm)和高剂量组(100 μm)及不含塞来昔布的对照组(等量生理盐水). 采用四甲基偶氮唑蓝法检测不同浓度塞来昔布对SGC-7901细胞增殖的影响;用Hoechst 33258检测细胞凋亡情况;荧光定量PCR检测BAX及Bcl-2基因表达水平. 结果 0、25、50、100 μm浓度的塞来昔布培养基SGC-7901细胞浓度分别为0.98、0.76、0.61、0.47 m/L,随着塞来昔布浓度升高,细胞浓度减少( P<0.05). 0、25、50、100 μm浓度的塞来昔布处理后SGC-7901细胞有核分裂现象分别为:≤1个/视野、2~3个/视野、5~6个/视野、≥10个/视野. 中剂量组、高剂量组BAX mRNA表达量高于对照组及低剂量组(P<0.05),高剂量组BAX mRNA表达量高于中剂量组(P<0.05);中剂量组、高剂量组 Bcl-2 mRNA 表达量低于对照组及低剂量组(P<0.05),高剂量组 Bcl-2 mRNA 表达量低于中剂量组( P<0.05). 结论 塞来昔布对胃癌细胞SGC-7901 有明显的抑制增殖作用,其作用机制可能与促进凋亡基因BAX的表达、抑制Bcl-2的表达有关.
目的 觀察塞來昔佈對人胃癌細胞SGC-7901凋亡的影響併探討其機製. 方法 將人胃癌細胞SGC-7901細胞分為4組,分彆加入不同濃度塞來昔佈,低劑量組(25 μm)、中劑量組(50 μm)和高劑量組(100 μm)及不含塞來昔佈的對照組(等量生理鹽水). 採用四甲基偶氮唑藍法檢測不同濃度塞來昔佈對SGC-7901細胞增殖的影響;用Hoechst 33258檢測細胞凋亡情況;熒光定量PCR檢測BAX及Bcl-2基因錶達水平. 結果 0、25、50、100 μm濃度的塞來昔佈培養基SGC-7901細胞濃度分彆為0.98、0.76、0.61、0.47 m/L,隨著塞來昔佈濃度升高,細胞濃度減少( P<0.05). 0、25、50、100 μm濃度的塞來昔佈處理後SGC-7901細胞有覈分裂現象分彆為:≤1箇/視野、2~3箇/視野、5~6箇/視野、≥10箇/視野. 中劑量組、高劑量組BAX mRNA錶達量高于對照組及低劑量組(P<0.05),高劑量組BAX mRNA錶達量高于中劑量組(P<0.05);中劑量組、高劑量組 Bcl-2 mRNA 錶達量低于對照組及低劑量組(P<0.05),高劑量組 Bcl-2 mRNA 錶達量低于中劑量組( P<0.05). 結論 塞來昔佈對胃癌細胞SGC-7901 有明顯的抑製增殖作用,其作用機製可能與促進凋亡基因BAX的錶達、抑製Bcl-2的錶達有關.
목적 관찰새래석포대인위암세포SGC-7901조망적영향병탐토기궤제. 방법 장인위암세포SGC-7901세포분위4조,분별가입불동농도새래석포,저제량조(25 μm)、중제량조(50 μm)화고제량조(100 μm)급불함새래석포적대조조(등량생리염수). 채용사갑기우담서람법검측불동농도새래석포대SGC-7901세포증식적영향;용Hoechst 33258검측세포조망정황;형광정량PCR검측BAX급Bcl-2기인표체수평. 결과 0、25、50、100 μm농도적새래석포배양기SGC-7901세포농도분별위0.98、0.76、0.61、0.47 m/L,수착새래석포농도승고,세포농도감소( P<0.05). 0、25、50、100 μm농도적새래석포처리후SGC-7901세포유핵분렬현상분별위:≤1개/시야、2~3개/시야、5~6개/시야、≥10개/시야. 중제량조、고제량조BAX mRNA표체량고우대조조급저제량조(P<0.05),고제량조BAX mRNA표체량고우중제량조(P<0.05);중제량조、고제량조 Bcl-2 mRNA 표체량저우대조조급저제량조(P<0.05),고제량조 Bcl-2 mRNA 표체량저우중제량조( P<0.05). 결론 새래석포대위암세포SGC-7901 유명현적억제증식작용,기작용궤제가능여촉진조망기인BAX적표체、억제Bcl-2적표체유관.
Objective To observe the effect of celecoxib on apoptosis in human gastric cancer cell line SGC-7901 and its mechanism. Methods The human gastric cancer cells (SGC-7901 cells) were divided into four groups,including low dose group(25 μm),moderate dose group(50μm),high dose group(100μm) and control group without celecoxib(equal dose of normal saline).MTT assay was used to determine the effects of different concentrations of celecoxib on the proliferation of SGC-7901 cells.Hoechst 33258 was used to detect the apoptosis of SGC-7901 cells,and fluorescence quantitative PCR was used to determine the expression levels of BAX and Bcl-2.Results The concentrations of SGC-7901 cells in celecoxib culture medium with concentrations of 0,25,50 and 100μm were 0.98,0.76,0.61 and 0.47 m/L,respectively, and the concentrations of the cells decreased with increasing concentrations of celecoxib(P<0.05).The presented number of nuclear divisions of SGC-7901 cells induced by celecoxib with concentrations of 0,25,50 and 100μm were≤1/microscope visual field,2-3/microscope visual field,5-6/microscope visual field and ≥10/microscope visual field,respectively.The expression of BAX mRNA in the moderate dose group or high dose group was higher than that in the low dose group or control group(P<0.05),and the expression of BAX mRNA in the high dose group was higher than that in the moderate dose group (P<0.05).The expression of Bcl-2 mRNA in the moderate dose group or high dose group was lower than that in the low dose group or control group(P<0.05),and the expression of Bcl-2 mRNA in the high dose group was lower than that in the modrate dose group (P<0.05).Conclusion Celecoxib has significant inhibitory effects on the proliferation of gastric cancer SGC-7901 cells.And its mechanism might be related to the promotion of BAX gene expression and the inhibitory of Bcl-2 gene expression.
