CAJ | 학술논문

目的： Munc－18在中枢神经系统递质释放过程中具有重要作用，其控制着突触囊泡释放步骤的每一个环节。Munc－18功能异常与癫痫发病相关。本文主要探讨癫痫发作是否对小鼠海马内Munc－18蛋白的分布产生影响。方法将18只雄性成年昆明鼠分为三组，生理盐水组和癫痫组分别向腹腔注射生理盐水和海人藻酸（10mg／kg）溶液，对照组不做处理，观察小鼠的行为变化。观察结束后小鼠全部处死取脑，冰冻切片行免疫组织化学染色后观察Munc－18蛋白和c－Fos蛋白的表达情况。结果注射海人藻酸30min后小鼠癫痫样发作，首先出现胡须和嘴部抽动，接着翘尾、甩尾、点头、单侧上肢抽搐，达到4级标准。与对照组和生理盐水组比较，癫痫组小鼠海马区域的Munc－18蛋白分布减少同时伴有c－Fos蛋白的表达增加，以CA3和齿状回区域更加明显，差别具有统计学差异。结论癫痫能够导致Munc－18蛋白在海马区域分布减少，同时使该区域的神经兴奋性增高。
목적： Munc－18재중추신경계통체질석방과정중구유중요작용，기공제착돌촉낭포석방보취적매일개배절。Munc－18공능이상여전간발병상관。본문주요탐토전간발작시부대소서해마내Munc－18단백적분포산생영향。방법장18지웅성성년곤명서분위삼조，생리염수조화전간조분별향복강주사생리염수화해인조산（10mg／kg）용액，대조조불주처리，관찰소서적행위변화。관찰결속후소서전부처사취뇌，빙동절편행면역조직화학염색후관찰Munc－18단백화c－Fos단백적표체정황。결과주사해인조산30min후소서전간양발작，수선출현호수화취부추동，접착교미、솔미、점두、단측상지추휵，체도4급표준。여대조조화생리염수조비교，전간조소서해마구역적Munc－18단백분포감소동시반유c－Fos단백적표체증가，이CA3화치상회구역경가명현，차별구유통계학차이。결론전간능구도치Munc－18단백재해마구역분포감소，동시사해구역적신경흥강성증고。
Objective Munc18-1 has an important role in neurotransmitter release which controls every step of release synap-tic vesicle.In the present study, whether epilepsy seizure causes a change of Munc-18 in hippocampus was investigated.Methods 18 KunMing mice were randomly divided into three groups:epilepsy group which was received intraperitoneal injection of Kainic acid (10mg/kg), saline group which was only received an injection of same amount of saline, control group which was received no treat-ment.Monitor the behavior of the mice, then the mice were slaughtered after investigation and the brain tissue was collected to perform frozen sections.Immunohistochemistry with anti-Munc-18 antibody was used to determine the distribution of Munc-18 protein in the hippocampus.Results 30 minutes after injection, behavioral changes of the mice appeared which including mouth and facial twit-ches, clonic head movements, unilateral forelimb clonus followed by contralateral clonus, clonic rearing.Compared with control group and saline group, the Munc-18 immunopositive cells were declined in CA3 and Dentate Gyrus of hippocampus;we also found that the expression of c-Fos proteins were significantly increased in the CA3 and Dentate Gyrus of hippocampus.Conclusion These results suggest that epilepsy seizures can induce the distribution change of Munc-18 in the hippocampus which may subsequently modulate neuronal functions in brain.