CAJ | 학술논문

目的：检测microRNA－33（miR－33）在过氧化氢（H2O2）诱导的NIH／3T3细胞衰老模型中的表达水平，并进一步研究过表达miR－33对H2 O2诱导的细胞衰老的影响。方法 H2 O2处理小鼠胚胎成纤维细胞系NIH／3T3后，利用SA－β－半乳糖苷酶染色以及Western blot检测p16蛋白的表达验证H2 O2诱导NIH／3T3细胞的衰老表型；利用荧光定量PCR检测miR－33在H2 O2诱导的衰老NIH／3T3细胞中的表达；利用RNAimax转染miR－33模拟物Agomir－33及其对照Agomir NC至NIH／3T3细胞，研究过表达miR－33对H2 O2诱导的NIH／3T3细胞衰老表型的影响。结果 miR－33在H2 O2诱导的NIH／3T3细胞衰老模型中显著下调，但过表达miR－33并不显著影响H2 O2诱导的NIH／3T3细胞衰老。结论 MicroRNA－33在过氧化氢诱导的细胞衰老表型中并不发挥显著作用。
목적：검측microRNA－33（miR－33）재과양화경（H2O2）유도적NIH／3T3세포쇠로모형중적표체수평，병진일보연구과표체miR－33대H2 O2유도적세포쇠로적영향。방법 H2 O2처리소서배태성섬유세포계NIH／3T3후，이용SA－β－반유당감매염색이급Western blot검측p16단백적표체험증H2 O2유도NIH／3T3세포적쇠로표형；이용형광정량PCR검측miR－33재H2 O2유도적쇠로NIH／3T3세포중적표체；이용RNAimax전염miR－33모의물Agomir－33급기대조Agomir NC지NIH／3T3세포，연구과표체miR－33대H2 O2유도적NIH／3T3세포쇠로표형적영향。결과 miR－33재H2 O2유도적NIH／3T3세포쇠로모형중현저하조，단과표체miR－33병불현저영향H2 O2유도적NIH／3T3세포쇠로。결론 MicroRNA－33재과양화경유도적세포쇠로표형중병불발휘현저작용。
Objective We herein attempted to detect the expression of microRNA-33 (miR-33) in the H2O2 -induced pre-mature senescence of NIH/3T3 cells, and further probed into the role of miR-33 on H2 O2 -induced premature senescence by ectopic expression of miR-33 in NIH/3T3 cells.Methods The senescence-associatedβ-galactosidase staining and the detection of mo-lecular senescencent marker, p16 expression levels by western blot were performed to confirm the premature senescence in NIH/3T3 cells after H2 O2 treatment;Real-time qPCR was utilized to detect the miR-33 expression in H2 O2 -induced premature senescence of NIH/3T3 cells;To investigate into the function of miR-33 on H2 O2 -induced premature senescence, miR-33 mimics ( Agomir 33) or the negative control ( Agomir NC) was trasfected into the NIH/3T3 cells by lipofectamine RNAimax.Results miR-33 was dramatically down-regulated in the H2 O2 -induced premature senescence of NIH/3T3 cells.Nonetheless, forced expression of miR-33 exhibited no significant effect on H2 O2 -induced premature senescence.Conclusion MicroRNA-33 might not play an important role in the H2 O2 -induced premature senescence.