CAJ | 학술논문

目的探讨静脉注射富氢水(HRS)是否能减轻牛磺胆酸钠诱导的重症急性胰腺炎(SAP)大鼠肺组织氧化应激反应,抑制炎症细胞因子.方法将54只健康SD雄性大鼠随机分成假手术组(Sham组)、模型组(SAP+NS组)和氢水处理组(SAP+HRS组),各组再按时间点分成6 h、12 h、24 h三个亚组,每个时间点处死6只大鼠.检测血清TNF-α、IL-1β含量;测定肺组织中髓过氧化物酶(MPO)活性以及TNF-α mRNA、IL-1β mRNA表达水平.结果(1)SAP+NS组和SAP+HRS组血清中TNF-α、IL-1β含量、肺组织中MPO活性、TNF-α mRNA、IL-1β mRNA的表达在6 h、12 h、24 h各个时间点均高于Sham组(P<0.05).(2)SAP+HRS组与SAP+NS组比较, SAP+HRS组血清TNF-α含量、肺组织TNF-α mRNA在各个时间点均低于SAP+NS组.血清IL-1β含量、肺组织中MPO活性、IL-1β mRNA表达在6 h时两组间无统计学差异,但在12 h、24 h时SAP+HRS组均低于SAP+NS组(P<0.05).结论静脉注射HRS能减轻牛磺胆酸钠诱导的SAP大鼠肺组织氧化应激反应,降低炎症细胞因子的水平.
목적 탐토정맥주사부경수(HRS)시부능감경우광담산납유도적중증급성이선염(SAP)대서폐조직양화응격반응,억제염증세포인자.방법 장54지건강SD웅성대서수궤분성가수술조(Sham조)、모형조(SAP+NS조)화경수처리조(SAP+HRS조),각조재안시간점분성6 h、12 h、24 h삼개아조,매개시간점처사6지대서.검측혈청TNF-α、IL-1β함량;측정폐조직중수과양화물매(MPO)활성이급TNF-α mRNA、IL-1β mRNA표체수평.결과(1)SAP+NS조화SAP+HRS조혈청중TNF-α、IL-1β함량、폐조직중MPO활성、TNF-α mRNA、IL-1β mRNA적표체재6 h、12 h、24 h각개시간점균고우Sham조(P<0.05).(2)SAP+HRS조여SAP+NS조비교, SAP+HRS조혈청TNF-α함량、폐조직TNF-α mRNA재각개시간점균저우SAP+NS조.혈청IL-1β함량、폐조직중MPO활성、IL-1β mRNA표체재6 h시량조간무통계학차이,단재12 h、24 h시SAP+HRS조균저우SAP+NS조(P<0.05).결론 정맥주사HRS능감경우광담산납유도적SAP대서폐조직양화응격반응,강저염증세포인자적수평.
Objective To discuss the effect of inflammatory cytokines and lung oxidative stress by intravenous injection of hydrogen-rich saline for sodium taurocholate induction of severe acute pancreatitis associated lung injury in rats.Methods 54 healthy male SD rats were randomly divided into sham-operated group (Sham), model group (SAP+NS) and hydrogen water treatment group (SAP+HRS), and each group was subdivided into 6 h, 12 h, 24 h three time points. The contents of TNF-α, IL-1β in the serum, the activity of myeloperoxidase, expression of TNF-α mRNA, IL-1β mRNA in the lung tissue were detected.Results The contents of TNF-α, IL-1β in the serum, MPO activity, TNF-α mRNA, IL-1β mRNA in the lung tissue in SAP+NS group and SAP+HRS group were higher than sham group at 6 h, 12 h, 24 h each time point (P<0.05). Compared with SAP+NS group, the contents of TNF-α in the serum, TNF-α mRNA in the lung tissue in SAP+HRS group were lower than SAP+NS group at all time points (P<0.05); the contents of IL-1β in the serum, MPO activity and IL-1β mRNA expression in the lung tissue were no statistical difference at the time of 6 h between SAP+NS group and SAP+HRS group, but were lower in SAP+HRS group than SAP+NS group at the time of 12 h, 24 h (P<0.05).Conclusion Intravenous HRS can reduce oxidative stress reaction and the levels of inflammatory cytokines of sodium taurocholate induction of severe acute pancreatitis associated lung injury in rats.