中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
Chinese Journal of Clinicians(Electronic Edition)
2015年
18期
3389-3393
,共5页
韩小妮%周静%孙玉芳%戴星%胡鸣%焦榕芳%方念
韓小妮%週靜%孫玉芳%戴星%鬍鳴%焦榕芳%方唸
한소니%주정%손옥방%대성%호명%초용방%방념
卵巢肿瘤%细胞增殖%细胞凋亡%miR-153
卵巢腫瘤%細胞增殖%細胞凋亡%miR-153
란소종류%세포증식%세포조망%miR-153
Ovarian neoplasms%Cell proliferation%Apoptosis%miR-153
目的 分析miR-153在卵巢癌中的表达,并探讨miR-153的生物学作用.方法 荧光定量 PCR 法检测卵巢癌组织及细胞中 miR-153 的表达水平;转染 miR-153 类似物(mimics)至SKOV3细胞,MTT法、平板克隆实验及流式细胞仪分别检测miR-153对细胞增殖及凋亡的影响.结果miR-153在卵巢癌组织及细胞中的表达显著下调;转染miR-153 mimics后细胞生长受到明显抑制,克隆数目减少,细胞早期凋亡率显著增加(P<0.01).结论 miR-153在卵巢癌中表达下调;过表达miR-153可显著抑制SKOV3细胞增殖,促进细胞凋亡.
目的 分析miR-153在卵巢癌中的錶達,併探討miR-153的生物學作用.方法 熒光定量 PCR 法檢測卵巢癌組織及細胞中 miR-153 的錶達水平;轉染 miR-153 類似物(mimics)至SKOV3細胞,MTT法、平闆剋隆實驗及流式細胞儀分彆檢測miR-153對細胞增殖及凋亡的影響.結果miR-153在卵巢癌組織及細胞中的錶達顯著下調;轉染miR-153 mimics後細胞生長受到明顯抑製,剋隆數目減少,細胞早期凋亡率顯著增加(P<0.01).結論 miR-153在卵巢癌中錶達下調;過錶達miR-153可顯著抑製SKOV3細胞增殖,促進細胞凋亡.
목적 분석miR-153재란소암중적표체,병탐토miR-153적생물학작용.방법 형광정량 PCR 법검측란소암조직급세포중 miR-153 적표체수평;전염 miR-153 유사물(mimics)지SKOV3세포,MTT법、평판극륭실험급류식세포의분별검측miR-153대세포증식급조망적영향.결과miR-153재란소암조직급세포중적표체현저하조;전염miR-153 mimics후세포생장수도명현억제,극륭수목감소,세포조기조망솔현저증가(P<0.01).결론 miR-153재란소암중표체하조;과표체miR-153가현저억제SKOV3세포증식,촉진세포조망.
Objective This study aims to analyze the miR-153 expression profile in ovarian cancer, explore the biological functions of miR-153.Methods The relative expression of miR-153 in ovarian cancer tissues and cancer cell lines were detected by real-time quantitative RT-PCR. Introduction of miR-153 mimics to SKOV3 cells. The cell proliferation ability were detected by MTT assay and colony formation, and cell apoptosis was detected by flow cytometry.Results The expression of miR-153 was significantly decreased in ovarian cancer tissues and cell lines; introduction of miR-153 mimics into the SKOV3 cells inhibited cell proliferation, colony formation ability and induced apoptosis (P<0.01). Conclusion miR-153, which is significantly downregulated in ovarian cancer tissues and cell lines, inhibits cell proliferation and induces apoptosis.
