中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
Chinese Journal of Clinicians(Electronic Edition)
2015年
18期
3383-3388
,共6页
宫颈肿瘤%白细胞介素类%顺铂%淋巴结转移%mTOR%P70S6K
宮頸腫瘤%白細胞介素類%順鉑%淋巴結轉移%mTOR%P70S6K
궁경종류%백세포개소류%순박%림파결전이%mTOR%P70S6K
Uterine cervical neoplasms%Interleukins%Cisplatin%Lymph node metastasis%mTOR%P70S6K
目的 探讨重组质粒pDC316hIL-24基因联合顺铂通过mTOR/P70S6K信号通路抑制人宫颈癌siha细胞淋巴转移.方法 将造模成功的人宫颈癌siha细胞雌性裸鼠模型随机分为6组,每组7只:PBS对照组、pDC316hIL-24组、低剂量顺铂组、高剂量顺铂组、pDC316hIL-24+低剂量顺铂组、pDC316hIL-24+高剂量顺铂组.观察裸鼠一般状况及淋巴结转移情况,实验结束后采用聚合酶链反应(PCR)法测定移植瘤中IL-24 mRNA的表达,CK角蛋白检测获取的淋巴组织有否癌浸润,免疫荧光法检测转移淋巴组织中mTOR和P70S6K蛋白表达,并用Western blot印迹法对其表达做定量分析.结果 IL-24基因成功转染及表达;CK角蛋白检测获取的各组淋巴组织有癌浸润;干预后各组淋巴转移数有差异,其中联合组淋巴结转移数明显低于对照组(P<0.05),而IL-24+低剂量顺铂组、IL-24+高剂量顺铂组淋巴转移数无统计学差异(P>0.05);免疫荧光法成功检测到各组转移淋巴组织中 mTOR/P70S6K 的表达,采用 Western 印迹法进行定量分析,与其他组比较,联合组表达显著下降,差异有统计学意义(P<0.05),且mTOR与P70S6K表达呈正相关性(r=0.968, P<0.001).结论 IL-24+低剂量顺铂治疗能达到IL-24+高剂量顺铂的治疗效果;IL-24+低剂量顺铂能明显抑制宫颈癌siha细胞淋巴转移;IL-24联合顺铂在体内通过抑制mTOR/P70S6K信号通路的活性促进细胞凋亡,从而抑制人宫颈癌siha细胞的淋巴转移.
目的 探討重組質粒pDC316hIL-24基因聯閤順鉑通過mTOR/P70S6K信號通路抑製人宮頸癌siha細胞淋巴轉移.方法 將造模成功的人宮頸癌siha細胞雌性裸鼠模型隨機分為6組,每組7隻:PBS對照組、pDC316hIL-24組、低劑量順鉑組、高劑量順鉑組、pDC316hIL-24+低劑量順鉑組、pDC316hIL-24+高劑量順鉑組.觀察裸鼠一般狀況及淋巴結轉移情況,實驗結束後採用聚閤酶鏈反應(PCR)法測定移植瘤中IL-24 mRNA的錶達,CK角蛋白檢測穫取的淋巴組織有否癌浸潤,免疫熒光法檢測轉移淋巴組織中mTOR和P70S6K蛋白錶達,併用Western blot印跡法對其錶達做定量分析.結果 IL-24基因成功轉染及錶達;CK角蛋白檢測穫取的各組淋巴組織有癌浸潤;榦預後各組淋巴轉移數有差異,其中聯閤組淋巴結轉移數明顯低于對照組(P<0.05),而IL-24+低劑量順鉑組、IL-24+高劑量順鉑組淋巴轉移數無統計學差異(P>0.05);免疫熒光法成功檢測到各組轉移淋巴組織中 mTOR/P70S6K 的錶達,採用 Western 印跡法進行定量分析,與其他組比較,聯閤組錶達顯著下降,差異有統計學意義(P<0.05),且mTOR與P70S6K錶達呈正相關性(r=0.968, P<0.001).結論 IL-24+低劑量順鉑治療能達到IL-24+高劑量順鉑的治療效果;IL-24+低劑量順鉑能明顯抑製宮頸癌siha細胞淋巴轉移;IL-24聯閤順鉑在體內通過抑製mTOR/P70S6K信號通路的活性促進細胞凋亡,從而抑製人宮頸癌siha細胞的淋巴轉移.
목적 탐토중조질립pDC316hIL-24기인연합순박통과mTOR/P70S6K신호통로억제인궁경암siha세포림파전이.방법 장조모성공적인궁경암siha세포자성라서모형수궤분위6조,매조7지:PBS대조조、pDC316hIL-24조、저제량순박조、고제량순박조、pDC316hIL-24+저제량순박조、pDC316hIL-24+고제량순박조.관찰라서일반상황급림파결전이정황,실험결속후채용취합매련반응(PCR)법측정이식류중IL-24 mRNA적표체,CK각단백검측획취적림파조직유부암침윤,면역형광법검측전이림파조직중mTOR화P70S6K단백표체,병용Western blot인적법대기표체주정량분석.결과 IL-24기인성공전염급표체;CK각단백검측획취적각조림파조직유암침윤;간예후각조림파전이수유차이,기중연합조림파결전이수명현저우대조조(P<0.05),이IL-24+저제량순박조、IL-24+고제량순박조림파전이수무통계학차이(P>0.05);면역형광법성공검측도각조전이림파조직중 mTOR/P70S6K 적표체,채용 Western 인적법진행정량분석,여기타조비교,연합조표체현저하강,차이유통계학의의(P<0.05),차mTOR여P70S6K표체정정상관성(r=0.968, P<0.001).결론 IL-24+저제량순박치료능체도IL-24+고제량순박적치료효과;IL-24+저제량순박능명현억제궁경암siha세포림파전이;IL-24연합순박재체내통과억제mTOR/P70S6K신호통로적활성촉진세포조망,종이억제인궁경암siha세포적림파전이.
Objective To investigate the inhibition of IL-24 in combination with cisplatin on human uterine lymph node metastasis.Methods Nude mice xenograft models were established with human cervical cancer siha cells, and then animal models were randomly divided into 6 groups with 7 mice in each group:PBS, IL-24, low-dose DDP, high-dose DDP, IL-24+low dose DDP and IL-24+high-dose DDP. At the end of the trial, from the conditions of weights and tumor growth to nude mice, PCR technique was tested to the expression of mRNA of IL-24 in transplantation tumor. In addition, there was a detection that whether lymphoid tissue was transplanted tumor lymphatic metastasis by CK keratin. Finally, the expression of mTOR and P70S6K were tested by immunofluorescence technique as well as Western blot imprinting method to quantitative analysis of its expression.Results IL-24 was successfully transfected and expressed in nude mice xenograft models, the CK keratin for each group was successfully tested in carcinoma infiltrating lymphoid tissues, after intervention, the numbers of metastasis lymph node in different groups were different, that is, the combined group was obvious lower than others (P<0.05), moreover, there was no statistically significant difference (P>0.05) on IL-24+low-dose cisplatin group and IL-24+high-dose cisplatin. mTOR/P70S6K was successfully tested in all of groups by immunofluorescence technique, and quantified by Western blot analysis, compared with other groups, the combined group was significantly decreased (P<0.05), the difference was statistically significant, and the expression of each two proteins had significant positive correlation (r=0.968,P<0.001).Conclusions The therapeutic effect on IL-24+low-dose DDP group is same with IL-24+high-dose DDP, moreover, IL-24+low-does DDP can significantly inhibit tumor growth and lymph number of metastasis. It is concluded that IL-24 combined with DDP in body inhibit the activity of mTOR/P70S6K signaling pathway to promote cell apoptosis signaling, from which human cervical cancer cells would be inhibited in nude mice growth and lymph node metastasis.
