CAJ | 학술논문

目的：观察辛伐他汀对脑出血大鼠神经功能的保护作用，并探讨其机制。方法将60只雄性SD大鼠随机分为假手术组、模型组和观察组，各20只。模型组和观察组右侧脑组织基底节内注入新鲜自体不凝血制作脑出血模型，假手术组注入等体积生理盐水。观察组术后完全苏醒后予辛伐他汀3 mg／（ kg· d）灌胃，模型组和假手术组等量生理盐水灌胃。术后48 h行神经功能障碍评分，计数神经元内尼氏小体数量观察神经细胞功能，用干湿重法检测血肿周围脑组织含水量，用Western blot检测血肿周围脑组织中的Toll样受体4（ TLR4）和IL-6。结果与假手术组相比，模型组神经功能障碍评分和神经元内尼氏小体数量下降，血肿周围脑组织含水量及TLR4、IL-6蛋白相对表达量升高，P均＜0．05；与模型组比较，观察组神经功能障碍评分和神经元内尼氏小体数量增加，血肿周围脑组织含水量及TLR4、IL-6蛋白相对表达量降低，P均＜0．05。结论辛伐他汀对脑出血大鼠具有脑保护作用；该作用可能与其降低血肿周围脑组织TLR4、IL-6表达，从而减轻继发性脑水肿及神经细胞损伤有关。
목적：관찰신벌타정대뇌출혈대서신경공능적보호작용，병탐토기궤제。방법장60지웅성SD대서수궤분위가수술조、모형조화관찰조，각20지。모형조화관찰조우측뇌조직기저절내주입신선자체불응혈제작뇌출혈모형，가수술조주입등체적생리염수。관찰조술후완전소성후여신벌타정3 mg／（ kg· d）관위，모형조화가수술조등량생리염수관위。술후48 h행신경공능장애평분，계수신경원내니씨소체수량관찰신경세포공능，용간습중법검측혈종주위뇌조직함수량，용Western blot검측혈종주위뇌조직중적Toll양수체4（ TLR4）화IL-6。결과여가수술조상비，모형조신경공능장애평분화신경원내니씨소체수량하강，혈종주위뇌조직함수량급TLR4、IL-6단백상대표체량승고，P균＜0．05；여모형조비교，관찰조신경공능장애평분화신경원내니씨소체수량증가，혈종주위뇌조직함수량급TLR4、IL-6단백상대표체량강저，P균＜0．05。결론신벌타정대뇌출혈대서구유뇌보호작용；해작용가능여기강저혈종주위뇌조직TLR4、IL-6표체，종이감경계발성뇌수종급신경세포손상유관。
Objective To observe the changes in the expression of TLR 4 and IL-6 in the rats after cerebral hemor-rhage,aswellastheinterventioneffectofsimvastatin.Method 60maleratswererandomlydividedintoshamgroup, model group and intervention group .To reproduce the model of cerebral hemorrhage , we injected the fresh autologous blood into the right side of the brain basal ganglia in the rats .The method of Garcia was used to evaluate the score of neurological dysfunction .The wet and dry weight method was used to detect the water content of brain tissue .The Nissl staining method was used to observe the pathological changes in the brain tissue , and count the number of intact neurons .The methods of immunohistochemistry and western blot were used to detect the expression level of TLR 4 and IL-6.Result Compared with sham operation group, the water content of brain in the model group was obviously higher (P<0.05), and the expression level of TLR4 and IL-6 around the hematoma increased (P<0.05), and accompany with severe neurological damage .Af-ter the treatment of simvastatin , both the expression level of TLR 4 and IL-6 around the hematoma and the water content of brain tissue decreased , accompanying with the up-regulation of the score of the neurological dysfunction .Conclusion Ac-tivated TLR4 after cerebral hemorrhage may participate the secondary inflammatory reaction and the pathological process of brain edema around the hematoma after cerebral hemorrhage via the activation of IL -6.Simvastatin may exert the protective effect of brain tissue via the improvement of the inflammatory response and the degree of brain edema mediated by TLR 4.