CAJ | 학술논문

目的:探讨癃畅颗粒对体外培养的人前列腺基质细胞增生及凋亡的影响.方法:原代培养增生的人前列腺基质细胞,分别以高、中、低浓度癃畅颗粒处理,MTT法测定细胞增殖指数,TUNEL法检测细胞凋亡.结果:癃畅颗粒处理实验组24 h后前列腺基质细胞的生长活性均被不同程度的抑制,高、中、低浓度组抗增殖指数分别为(0.273±0.0881)%,(0.418± 0.0644)%和(0.674±0.0352)%,与空白对照组比较均有显著差异(P<0.05);随着药物浓度的增加,细胞抗增殖指数逐渐升高,各浓度组间比较均有统计学差异(P<0.05);药物处理后细胞凋亡百分率显著增加,高、中、低浓度组凋亡指数分别为(22.12± 3.47)%,(44.96±2.81)%和(80.36±10.16)%,与空白对照组比较均有显著差异(P<0.05);随着药物浓度的增加,凋亡指数逐渐升高,各浓度组间比较均有统计学差异(P<0.05).结论:癃畅颗粒能够显著抑制体外培养的人前列腺基质细胞增殖,促进前列腺基质细胞凋亡.
목적:탐토륭창과립대체외배양적인전렬선기질세포증생급조망적영향.방법:원대배양증생적인전렬선기질세포,분별이고、중、저농도륭창과립처리,MTT법측정세포증식지수,TUNEL법검측세포조망.결과:륭창과립처리실험조24 h후전렬선기질세포적생장활성균피불동정도적억제,고、중、저농도조항증식지수분별위(0.273±0.0881)%,(0.418± 0.0644)%화(0.674±0.0352)%,여공백대조조비교균유현저차이(P<0.05);수착약물농도적증가,세포항증식지수축점승고,각농도조간비교균유통계학차이(P<0.05);약물처리후세포조망백분솔현저증가,고、중、저농도조조망지수분별위(22.12± 3.47)%,(44.96±2.81)%화(80.36±10.16)%,여공백대조조비교균유현저차이(P<0.05);수착약물농도적증가,조망지수축점승고,각농도조간비교균유통계학차이(P<0.05).결론:륭창과립능구현저억제체외배양적인전렬선기질세포증식,촉진전렬선기질세포조망.
Objective To investigate the effect of Longchang granules on the proliferation and apoptosis of prostate stromal cells in vitro and to provide the theoretical basis for Longchang granules to treat benign prostat-ic hyperplasia. Methods Longchang granules at low, medium and high doses were prepared and added to the prostate stromal cells in vitro. The proliferation of cells was tested by MTT. The apoptosis of cells was identified by TUNEL assay. Results After being treated for 24 h, there were statistical differences between blank group and longchang groups in the proliferation of prostate stromal cells (P<0.05) and there were also significant dif-ferences between high-dose,medium-dose and low-dose groups (P<0.05). The results tested by TUNEL assay showed that there were statistical differences between blank group and longchang groups in the apoptosis of pros-tate stromal cells (P<0.05), and there were also significant differences between high-dose,medium-dose and low-dose groups (P<0.05). Conclusion Longchang granules can significantly inhibit the proliferation and promote the apoptosis of prostate stromal cells in vitro.