中国药理学与毒理学杂志
中國藥理學與毒理學雜誌
중국약이학여독이학잡지
Chinese Journal of Pharmacology and Toxicology
2015年
5期
825-830
,共6页
陈艳%王盛丰%沈欢欢%龚婷婷%单伟光
陳豔%王盛豐%瀋歡歡%龔婷婷%單偉光
진염%왕성봉%침환환%공정정%단위광
元胡总碱%胃黏膜%消化性溃疡%表皮生长因子
元鬍總堿%胃黏膜%消化性潰瘍%錶皮生長因子
원호총감%위점막%소화성궤양%표피생장인자
total alkaloids of Rhizoma Corydalis%gastric mucosa%peptic ulcer%epidermal growth factor
目的：研究元胡总碱（TARC）对实验性大鼠胃黏膜损伤的保护作用。方法利用胃黏膜下注射冰乙酸方法制备大鼠急性胃黏膜损伤模型。第2天，按照分组分别ig给予西咪替丁400 mg·kg-1，TARC 20，40和80 mg·kg-1，连续ig 15 d，末次给药后2 d，测定大鼠胃液分泌量及胃液总酸度；HE染色观察胃组织病理变化，计算胃黏膜损伤指数和损伤抑制率；免疫组织化学法测定胃黏膜表皮生长因子（EGF）和EGF受体（EGFR）的表达水平。结果与假手术组比较，模型组大鼠胃黏膜损伤面积和损伤指数显著增加（P<0.01），提示大鼠胃黏膜损伤模型制备成功。与模型组比较，西咪替丁组和TARC 80 mg·kg-1组分别降低胃黏膜损伤面积的39.9%和23.7%，胃黏膜损伤指数分别降低52.3%和30.5%（P<0.01），TARC 80 mg·kg-1组与西咪替丁组比较无统计学差异。与模型组比较，西咪替丁组及TARC 40和80 mg·kg-1组大鼠胃黏膜EGF蛋白表达分别增加81.8%，24.2%和57.6%，EGFR蛋白表达分别增加45.9，16.2%和29.7%。病理学观察发现，模型组胃黏膜脱落坏死，大量炎性细胞浸润；西咪替丁组、TARC 40和80 mg·kg-1组胃组织病变明显改善。结论 TARC具有保护大鼠急性胃黏膜损伤的作用，其机制可能与上调胃黏膜组织中EGF和EGFR的表达有关。
目的：研究元鬍總堿（TARC）對實驗性大鼠胃黏膜損傷的保護作用。方法利用胃黏膜下註射冰乙痠方法製備大鼠急性胃黏膜損傷模型。第2天，按照分組分彆ig給予西咪替丁400 mg·kg-1，TARC 20，40和80 mg·kg-1，連續ig 15 d，末次給藥後2 d，測定大鼠胃液分泌量及胃液總痠度；HE染色觀察胃組織病理變化，計算胃黏膜損傷指數和損傷抑製率；免疫組織化學法測定胃黏膜錶皮生長因子（EGF）和EGF受體（EGFR）的錶達水平。結果與假手術組比較，模型組大鼠胃黏膜損傷麵積和損傷指數顯著增加（P<0.01），提示大鼠胃黏膜損傷模型製備成功。與模型組比較，西咪替丁組和TARC 80 mg·kg-1組分彆降低胃黏膜損傷麵積的39.9%和23.7%，胃黏膜損傷指數分彆降低52.3%和30.5%（P<0.01），TARC 80 mg·kg-1組與西咪替丁組比較無統計學差異。與模型組比較，西咪替丁組及TARC 40和80 mg·kg-1組大鼠胃黏膜EGF蛋白錶達分彆增加81.8%，24.2%和57.6%，EGFR蛋白錶達分彆增加45.9，16.2%和29.7%。病理學觀察髮現，模型組胃黏膜脫落壞死，大量炎性細胞浸潤；西咪替丁組、TARC 40和80 mg·kg-1組胃組織病變明顯改善。結論 TARC具有保護大鼠急性胃黏膜損傷的作用，其機製可能與上調胃黏膜組織中EGF和EGFR的錶達有關。
목적：연구원호총감（TARC）대실험성대서위점막손상적보호작용。방법이용위점막하주사빙을산방법제비대서급성위점막손상모형。제2천，안조분조분별ig급여서미체정400 mg·kg-1，TARC 20，40화80 mg·kg-1，련속ig 15 d，말차급약후2 d，측정대서위액분비량급위액총산도；HE염색관찰위조직병리변화，계산위점막손상지수화손상억제솔；면역조직화학법측정위점막표피생장인자（EGF）화EGF수체（EGFR）적표체수평。결과여가수술조비교，모형조대서위점막손상면적화손상지수현저증가（P<0.01），제시대서위점막손상모형제비성공。여모형조비교，서미체정조화TARC 80 mg·kg-1조분별강저위점막손상면적적39.9%화23.7%，위점막손상지수분별강저52.3%화30.5%（P<0.01），TARC 80 mg·kg-1조여서미체정조비교무통계학차이。여모형조비교，서미체정조급TARC 40화80 mg·kg-1조대서위점막EGF단백표체분별증가81.8%，24.2%화57.6%，EGFR단백표체분별증가45.9，16.2%화29.7%。병이학관찰발현，모형조위점막탈락배사，대량염성세포침윤；서미체정조、TARC 40화80 mg·kg-1조위조직병변명현개선。결론 TARC구유보호대서급성위점막손상적작용，기궤제가능여상조위점막조직중EGF화EGFR적표체유관。
OBJECTIVE To investigate the protective effect of total alkaloids of Rhizoma Corydalis (TARC) on experimental gastric mucosal lesions in rats. METHODS Gastric mucosal lesions were induced in rats by injecting acetic acid under gastric mucosal. From the 2nd day post the preparation of the rat model, cimetidine 400 mg · kg-1 or TARC 20, 40 and 80 mg · kg-1 was ig delivered for 15 d in different groups. Two days after the last delivery, gastric juice volume and total acidity were measured. Histopathology of stomach tissues was observed by HE staining. The area of gastric ulcer area was measured and the ulcer index and ulcer inhibitory rate were calculated. The expression of epidermal growth factor (EGF) and EGF receptor (EGFR) was detected by immunohistochemical staining. RESULTS Comparing with shame group, the eare of gastric ulcer and ulcer index were increased signifi?cantly in the model group(P<0.01), suggesting that the model rats were prepared properly. Compared with the model group, the ulcer area in rats of cimetidine and TARC 80 mg·kg-1 groups was decreased by 39.9%and 23.7%,respectively. The ulcer index was decreased by 52.3%and 30.5%,respectively. There was no significant difference between the cimetidine group and TARC 80 mg · kg-1 group, in the ulcer area or index. Compared with model group, EGF protein expression of cimetidine and TARC 40 and 80 mg · kg-1 groups was increased by 81.8%,24.2%and 57.6%,respectively while EGFR protein expression was increased by 45.9%,16.2%and 29.7%,respectively(P<0.05,P<0.01). Deciduous and necrotic gastric mucosal and a large amount of inflmmatory cells infiltration were observed in model group, and gastric mucosal lesions were improved in cimetidine and TARC 40 and 80 mg · kg-1 groups. CONCLUSION TARC has protective effect on gastric mucosal lesions in rats. The mechanism may be related to the increase of EGF and EGFR protein expression.
