中国土壤与肥料
中國土壤與肥料
중국토양여비료
Soils and Fertilizers Sciences in China
2015年
5期
116-121
,共6页
王泽%徐齐%袁梅%张磊%高淼%孙建光
王澤%徐齊%袁梅%張磊%高淼%孫建光
왕택%서제%원매%장뢰%고묘%손건광
菠菜内生固氮菌%假单胞菌属%功能特性%多功能菌
菠菜內生固氮菌%假單胞菌屬%功能特性%多功能菌
파채내생고담균%가단포균속%공능특성%다공능균
spinach endogenous nitrogen-fixing bacteria%Pseudomonas%functional characterizations%multifunctional bacterium
内生固氮菌具有促生、 病原菌的防治及生物固氮等作用, 并且占据着植物组织内有利于营养供应和微环境适宜的生态位, 是重要的微生物菌种资源. 从菠菜根内分离筛选优势内生固氮菌7 株, 其中假单胞菌属( Pseudomonas) 5株, 红球菌属 ( Rhodococcus) 和黄杆菌属 ( Flavobacterium) 各1株. 菌株Pseudomonas sp. BC-E6和Pseudomonas sp. BC-E8固氮酶活性较高, 分别为 (13. 19 ± 0. 32) 和 (12. 11 ± 0. 96) C2 H4 nmol/ ( mg pro-tein·h) , 与圆褐固氮菌 ( ACCC11103 ) 的固氮酶活性具有极显著性差异 ( P <0. 01 ); 菌株 Pseudomonas sp. BC-E7具有固氮酶活性 [ (8. 42 ±0. 03) C2H4 nmol/ (mg protein·h)]、 产IAA能力 [ (59. 58 ±4. 15) μg/mL]、ACC脱氨酶活性 [ (5. 067 ±0. 376) μmol/ (mg protein·h)], 拮抗立枯丝核菌ACCC36124 (Rhizoctonia solani)、麦类赤霉病ACCC36272 ( Gibberella zeae) 等4种常见土传病害. 菠菜内生固氮菌Pseudomonas sp. BC-E7是一株多功能内生菌, 具有较好的生产应用前景.
內生固氮菌具有促生、 病原菌的防治及生物固氮等作用, 併且佔據著植物組織內有利于營養供應和微環境適宜的生態位, 是重要的微生物菌種資源. 從菠菜根內分離篩選優勢內生固氮菌7 株, 其中假單胞菌屬( Pseudomonas) 5株, 紅毬菌屬 ( Rhodococcus) 和黃桿菌屬 ( Flavobacterium) 各1株. 菌株Pseudomonas sp. BC-E6和Pseudomonas sp. BC-E8固氮酶活性較高, 分彆為 (13. 19 ± 0. 32) 和 (12. 11 ± 0. 96) C2 H4 nmol/ ( mg pro-tein·h) , 與圓褐固氮菌 ( ACCC11103 ) 的固氮酶活性具有極顯著性差異 ( P <0. 01 ); 菌株 Pseudomonas sp. BC-E7具有固氮酶活性 [ (8. 42 ±0. 03) C2H4 nmol/ (mg protein·h)]、 產IAA能力 [ (59. 58 ±4. 15) μg/mL]、ACC脫氨酶活性 [ (5. 067 ±0. 376) μmol/ (mg protein·h)], 拮抗立枯絲覈菌ACCC36124 (Rhizoctonia solani)、麥類赤黴病ACCC36272 ( Gibberella zeae) 等4種常見土傳病害. 菠菜內生固氮菌Pseudomonas sp. BC-E7是一株多功能內生菌, 具有較好的生產應用前景.
내생고담균구유촉생、 병원균적방치급생물고담등작용, 병차점거착식물조직내유리우영양공응화미배경괄의적생태위, 시중요적미생물균충자원. 종파채근내분리사선우세내생고담균7 주, 기중가단포균속( Pseudomonas) 5주, 홍구균속 ( Rhodococcus) 화황간균속 ( Flavobacterium) 각1주. 균주Pseudomonas sp. BC-E6화Pseudomonas sp. BC-E8고담매활성교고, 분별위 (13. 19 ± 0. 32) 화 (12. 11 ± 0. 96) C2 H4 nmol/ ( mg pro-tein·h) , 여원갈고담균 ( ACCC11103 ) 적고담매활성구유겁현저성차이 ( P <0. 01 ); 균주 Pseudomonas sp. BC-E7구유고담매활성 [ (8. 42 ±0. 03) C2H4 nmol/ (mg protein·h)]、 산IAA능력 [ (59. 58 ±4. 15) μg/mL]、ACC탈안매활성 [ (5. 067 ±0. 376) μmol/ (mg protein·h)], 길항립고사핵균ACCC36124 (Rhizoctonia solani)、맥류적매병ACCC36272 ( Gibberella zeae) 등4충상견토전병해. 파채내생고담균Pseudomonas sp. BC-E7시일주다공능내생균, 구유교호적생산응용전경.
Endogenous nitrogen-fixing bacteria have positive functions of promoting growth, pathogen prevention and biological nitrogen fixation and occupy the plant tissue which is conductive to nutrient supply and suitable micro ecological environment. In this study, seven endogenous nitrogen-fixing bacteria were isolated from the root of spinach. Among the 7 diazotrophic bac-teria, five were identified as Pseudomonas and two belonged to Rhodococcus and Flavobacterium, respecitively. The nitroge-nase activities of strain Pseudomonas sp. BC-E6 and Pseudomonas sp. BC-E8 were ( 13. 19 ± 0. 32 ) and ( 12. 11 ± 0. 96 ) C2H4 nmol/(mg protein ·h), which was significantly higher (P<0. 01) than that of Azotobacter chroococcum ACCC11104. For Strain Pseudomonas sp. BC-E7, the nitrogenase activity was (8.42 ±0.03) C2H4 nmol/(mg protein ·h), production of indole acetic acid (IAA) was (59. 58 ± 4. 15) μg/mL, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity was (5. 067 ± 0. 376) μmol/(mg protein·h), and it had the capability to antagonize the growth of Rhizoctonia solani ACCC36124, Gibberella zeae ACCC362724, Sclerotinia sclerotiorum ACCC36084 and Sclerotinia sclerotiorum ACCC36905. Strain Pseudomonas sp. BC-E7 had been a multifunctional endogenous nitrogen-fixing bacterium, which should have good application prospect.
