畜牧与饲料科学
畜牧與飼料科學
축목여사료과학
Animal Husbandry and Feed Science
2015年
10期
10-13,22
,共5页
巨玉鑫%赵德宝%李晓梅%马剑青%王旭刚%史兆国
巨玉鑫%趙德寶%李曉梅%馬劍青%王旭剛%史兆國
거옥흠%조덕보%리효매%마검청%왕욱강%사조국
白羽肉鸡%黄羽肉鸡%免疫器官%组织结构
白羽肉鷄%黃羽肉鷄%免疫器官%組織結構
백우육계%황우육계%면역기관%조직결구
white feather broilers%yellow feather broilers%immune organs%histological structure
取42日龄白羽肉鸡和黄羽肉鸡的法氏囊、脾脏及胸腺,运用组织学常规切片技术和HE染色法,制作石蜡切片,在光学显微镜下逐一观察其组织结构,并对其进行显微照相,比较白羽肉鸡和黄羽肉鸡法氏囊、脾脏及胸腺组织形态的差异。结果表明,42日龄时,与黄羽肉鸡相比,白羽肉鸡法氏囊小结数量较少,皮质和髓质的分界更难分辨,并且固有层中出现大量空泡和间质结缔组织,淋巴细胞数量减少,表明免疫功能在减退;白羽肉鸡脾脏的白髓和红髓较明显,脾小结明显增大,脾小结内的淋巴细胞数量增加,排列密集,免疫功能较强;白羽肉鸡胸腺皮质部的强嗜碱性着染的小淋巴细胞较多,髓质内有更多典型的胸腺小体。这表明,同日龄的白羽肉鸡免疫器官的发育速度和功能建立要比黄羽肉鸡快,二者具有品种间差异。
取42日齡白羽肉鷄和黃羽肉鷄的法氏囊、脾髒及胸腺,運用組織學常規切片技術和HE染色法,製作石蠟切片,在光學顯微鏡下逐一觀察其組織結構,併對其進行顯微照相,比較白羽肉鷄和黃羽肉鷄法氏囊、脾髒及胸腺組織形態的差異。結果錶明,42日齡時,與黃羽肉鷄相比,白羽肉鷄法氏囊小結數量較少,皮質和髓質的分界更難分辨,併且固有層中齣現大量空泡和間質結締組織,淋巴細胞數量減少,錶明免疫功能在減退;白羽肉鷄脾髒的白髓和紅髓較明顯,脾小結明顯增大,脾小結內的淋巴細胞數量增加,排列密集,免疫功能較彊;白羽肉鷄胸腺皮質部的彊嗜堿性著染的小淋巴細胞較多,髓質內有更多典型的胸腺小體。這錶明,同日齡的白羽肉鷄免疫器官的髮育速度和功能建立要比黃羽肉鷄快,二者具有品種間差異。
취42일령백우육계화황우육계적법씨낭、비장급흉선,운용조직학상규절편기술화HE염색법,제작석사절편,재광학현미경하축일관찰기조직결구,병대기진행현미조상,비교백우육계화황우육계법씨낭、비장급흉선조직형태적차이。결과표명,42일령시,여황우육계상비,백우육계법씨낭소결수량교소,피질화수질적분계경난분변,병차고유층중출현대량공포화간질결체조직,림파세포수량감소,표명면역공능재감퇴;백우육계비장적백수화홍수교명현,비소결명현증대,비소결내적림파세포수량증가,배렬밀집,면역공능교강;백우육계흉선피질부적강기감성착염적소림파세포교다,수질내유경다전형적흉선소체。저표명,동일령적백우육계면역기관적발육속도화공능건립요비황우육계쾌,이자구유품충간차이。
The paraffin sections of bursa, spleen and thymus of 42-day-old white feather broilers and yellow feather broilers were prepared by using conventional histological slicing technique and HE staining method. The histological structures of them were observed and recorded by microphotography method, and the morphological variations of bursa, spleen and thymus tissues between 42-day-old white feather broilers and yellow feather broilers were compared. The results showed that compared with 42-day-old yellow feather broilers, the bursa lobules of white feather broilers with the same age were fewer, their cortex and medulla were more difficult to distinguish, massive vacuoles and interstitial connective tissue were distributed in lamina propria, and there were decreased numbers of lymphocytes, indicating that the immunologic function were declined. The white pulp and red pulp of white feather broilers spleen were obvious, the splenic corpuscle were markedly enlarged, the number of lymphocytes were increased and packed tightly, indicating the strong immunologic function. There were numerous basophilic small lymphocytes in cortex of white feather broilers thymus and more typical thymic corpuscle in medulla. The results of the study indicate that the rate of development and function establishment of immune organs of white feather broilers are earlier than those of immune organs of yellow feather broilers with the same age, and there are histological differences between the immune organs of the two species.