临床眼科杂志
臨床眼科雜誌
림상안과잡지
Journal of Clinical Ophthalmology
2015年
5期
461-463,464
,共4页
视网膜%Müller细胞%葡萄糖%谷氨酰胺合成酶%重组人促红细胞生成素
視網膜%Müller細胞%葡萄糖%穀氨酰胺閤成酶%重組人促紅細胞生成素
시망막%Müller세포%포도당%곡안선알합성매%중조인촉홍세포생성소
Retina%Müller cells%Glucose%Glutamine synthetase%Recombinant human erythropoietin
目的：探讨重组人促红细胞生成素对高浓度葡萄糖培养大鼠视网膜Müller细胞功能的影响。方法体外传代培养大鼠视网膜Müller细胞，分组为N组（正常对照），G25组（25 mmol／L葡萄糖），G50组（50 mmol／L葡萄糖），EG25组（4×104IU／L rhEPO＋25 mmol／L葡萄糖），EG50组（4×104IU／L rhEPO＋50 mmol／L葡萄糖），MTT检测各组细胞活性。细胞免疫染色、ELISA检测各组细胞GS蛋白表达的情况。结果 MTT检测示N组细胞活性高于G25组、G50组、EG25组和EG50组，EG25组高于G25组，EG50组高于G50组。细胞免疫荧光染色检测各组细胞均有GS蛋白阳性着染。酶联免疫吸附试验检测N组GS蛋白表达高于各高浓度葡萄糖培养组，EG25组、EG50组蛋白表达分别高于G25组和G50组。结论重组人促红细胞生成素可增加高浓度葡萄糖培养大鼠视网膜Müller细胞活性并上调GS蛋白的表达。
目的：探討重組人促紅細胞生成素對高濃度葡萄糖培養大鼠視網膜Müller細胞功能的影響。方法體外傳代培養大鼠視網膜Müller細胞，分組為N組（正常對照），G25組（25 mmol／L葡萄糖），G50組（50 mmol／L葡萄糖），EG25組（4×104IU／L rhEPO＋25 mmol／L葡萄糖），EG50組（4×104IU／L rhEPO＋50 mmol／L葡萄糖），MTT檢測各組細胞活性。細胞免疫染色、ELISA檢測各組細胞GS蛋白錶達的情況。結果 MTT檢測示N組細胞活性高于G25組、G50組、EG25組和EG50組，EG25組高于G25組，EG50組高于G50組。細胞免疫熒光染色檢測各組細胞均有GS蛋白暘性著染。酶聯免疫吸附試驗檢測N組GS蛋白錶達高于各高濃度葡萄糖培養組，EG25組、EG50組蛋白錶達分彆高于G25組和G50組。結論重組人促紅細胞生成素可增加高濃度葡萄糖培養大鼠視網膜Müller細胞活性併上調GS蛋白的錶達。
목적：탐토중조인촉홍세포생성소대고농도포도당배양대서시망막Müller세포공능적영향。방법체외전대배양대서시망막Müller세포，분조위N조（정상대조），G25조（25 mmol／L포도당），G50조（50 mmol／L포도당），EG25조（4×104IU／L rhEPO＋25 mmol／L포도당），EG50조（4×104IU／L rhEPO＋50 mmol／L포도당），MTT검측각조세포활성。세포면역염색、ELISA검측각조세포GS단백표체적정황。결과 MTT검측시N조세포활성고우G25조、G50조、EG25조화EG50조，EG25조고우G25조，EG50조고우G50조。세포면역형광염색검측각조세포균유GS단백양성착염。매련면역흡부시험검측N조GS단백표체고우각고농도포도당배양조，EG25조、EG50조단백표체분별고우G25조화G50조。결론중조인촉홍세포생성소가증가고농도포도당배양대서시망막Müller세포활성병상조GS단백적표체。
Objective Present study was to investigate the effect of recombinant human erythropoietin on retinal Müller cells cultured in high glucose.Methods Serial subcultivated neonatal rats’ retinal Müller cells were divided into normal control group, G25(25mmol/Lglucose) group, G50(50mmol/Lglucose), EG25(4 ×104 IU/L recombinant human erythropoietin+25mmol/L glucose) group and EG50 (4 ×104 IU/L recombinant human erythropoietin +50mmol/Lglucose) group.MTT assay was applied to detect cell viability in five groups.The expression of glutamine synthetase was measured by immunofluorescence and enzyme linked immunosorbent assay.Results Cell viability of G25 group, G50 group, EG25 group and EG50 group was lower than that of normal group.Cell viability of EG25 group was higher than that of G25 group, and EG50 group was higher than that of G50 group.Cells expressed glutamine synthetase in five groups.The expression of glutamine synthetase in normal group was higher than that in other four groups where cells were cultured in high glucose. The expression of glutamine synthetase in EG25 group and EG50 group were higher than that in G25 group and G50 group re-spectively.Conclusion Recombinant human erythropoietin could increase the cell viability and the expression of gluta-mine synthetase in retinal Müller cells cultured in high glucose.
