CAJ | 학술논문

目的初步研究调控雪旺细胞迁移的信号传导通路.方法细胞划痕实验对比静息态与激活态雪旺细胞迁移能力,用Western blot方法测定各自ERK1/2和AKT蛋白表达.应用相应蛋白通路抑制剂后观察雪旺细胞迁移能力改变及ERK1/2和AKT蛋白表达,初步探索雪旺细胞迁移信号通路.结果激活态雪旺细胞迁移能力较静息雪旺细胞明显提高,同时伴随着p-ERK表达增加.单纯阻断ERK磷酸化对激活态雪旺细胞迁移的能力无明显影响,而且伴随着p-AKT表达增加.联合阻断ERK及AKT磷酸化可明显降低激活态雪旺细胞的迁移能力.结论雪旺细胞的迁移能力与ERK及AKT通路联合调控相关.
목적 초보연구조공설왕세포천이적신호전도통로.방법 세포화흔실험대비정식태여격활태설왕세포천이능력,용Western blot방법측정각자ERK1/2화AKT단백표체.응용상응단백통로억제제후관찰설왕세포천이능력개변급ERK1/2화AKT단백표체,초보탐색설왕세포천이신호통로.결과 격활태설왕세포천이능력교정식설왕세포명현제고,동시반수착p-ERK표체증가.단순조단ERK린산화대격활태설왕세포천이적능력무명현영향,이차반수착p-AKT표체증가.연합조단ERK급AKT린산화가명현강저격활태설왕세포적천이능력.결론 설왕세포적천이능력여ERK급AKT통로연합조공상관.
Objective To explore the cell signaling pathway involved in Schwann cell (SCs) migration.Methods Quiescent Schwann cells (qSCs) and activated Schwann cells (aSCs) were cultured and then identified by imunnostaining of S100 and p75NGFR.Cell mobility was determined by the wound healing assay.Western blot was adopted to detect ERK1/2, ATK and their phosphorylation style.SC migration signaling pathway was explored by observing changes in cell mobility and ERK1/2 and AKT expression upon inhibition of the pathways.Results The mobility of aSCs was higher than that of qSCs.Elevated p-ERK1/2 was coupled with the migration of SCs, activated by nerve degeneration.Simple inhibition of ERK phosphorylation did not show significant negative effect on the migratory potential of aSCs, but elevation of p-AKT expression.Combined inhibition of ERK and AKT phosphorylation resulted in a significant decrease in aSCs mobility.Conclusion Both ERK and AKT pathways are involved in Schwann cell migration.