CAJ | 학술논문

目的：从细胞生物学及分子生物学水平探讨金水鲜冻干粉悬液联合顺铂处理后，宫颈癌C-33A细胞宫颈肿瘤细胞增殖能力和细胞周期分布的变化及其对细胞周期相关蛋白表达的影响。方法选择不同浓度(0.1、0.2、0.5、1.0 mg/mL)金水鲜冻干粉处理宫颈癌C-33A细胞，同时以0.9%氯化钠溶液作为对照，采用CCK8法检测不同浓度的金水鲜冻干粉作用12、24、48 h和96 h后宫颈癌C-33A细胞增殖的抑制率；分别检测0.9%氯化钠溶液、30μg/mL顺铂单药、最佳抑制率浓度的金水鲜冻干粉以及顺铂联合最佳抑制率浓度的金水鲜冻干粉处理48 h后宫颈癌C-33A细胞的增殖活力；采用Western Blot检测以上不同处理对细胞周期分布变化及周期相关蛋白p21、p53的表达量。结果 CCK8法显示，0.5 mg/mL浓度的金水鲜冻干粉单独处理宫颈癌细胞48 h时能最明显抑制细胞的增殖，抑制率达61.2%，与0.1 mg/mL和0.2 mg/mL金水鲜冻干粉比较(19.8%比37.6%)，差异均有高度统计学意义(P<0.01)；顺铂联合0.5 mg/mL金水鲜冻干粉处理48 h后宫颈癌C-33A细胞的抑制率达82.05%，与单纯使用顺铂抑制细胞增殖率(65.24%)比较，差异有统计学意义(P<0.05)；流式细胞仪细胞周期分析显示金水鲜冻干粉联合顺铂使细胞发生G1期阻滞，顺铂联合0.5 mg/mL金水鲜冻干粉作用48 h后p21、p53蛋白的的表达均明显升高，与0.9%氯化钠溶液及顺铂比较，差异均有统计学意义(P<0.05)。结论选择合适的金水鲜冻干粉剂量浓度可加强顺铂对宫颈癌细胞的抑制增殖并可阻滞细胞周期G1~S期进程，该生物学效应可能与促进p21/p53蛋白表达上调相关。
목적：종세포생물학급분자생물학수평탐토금수선동간분현액연합순박처리후，궁경암C-33A세포궁경종류세포증식능력화세포주기분포적변화급기대세포주기상관단백표체적영향。방법선택불동농도(0.1、0.2、0.5、1.0 mg/mL)금수선동간분처리궁경암C-33A세포，동시이0.9%록화납용액작위대조，채용CCK8법검측불동농도적금수선동간분작용12、24、48 h화96 h후궁경암C-33A세포증식적억제솔；분별검측0.9%록화납용액、30μg/mL순박단약、최가억제솔농도적금수선동간분이급순박연합최가억제솔농도적금수선동간분처리48 h후궁경암C-33A세포적증식활력；채용Western Blot검측이상불동처리대세포주기분포변화급주기상관단백p21、p53적표체량。결과 CCK8법현시，0.5 mg/mL농도적금수선동간분단독처리궁경암세포48 h시능최명현억제세포적증식，억제솔체61.2%，여0.1 mg/mL화0.2 mg/mL금수선동간분비교(19.8%비37.6%)，차이균유고도통계학의의(P<0.01)；순박연합0.5 mg/mL금수선동간분처리48 h후궁경암C-33A세포적억제솔체82.05%，여단순사용순박억제세포증식솔(65.24%)비교，차이유통계학의의(P<0.05)；류식세포의세포주기분석현시금수선동간분연합순박사세포발생G1기조체，순박연합0.5 mg/mL금수선동간분작용48 h후p21、p53단백적적표체균명현승고，여0.9%록화납용액급순박비교，차이균유통계학의의(P<0.05)。결론선택합괄적금수선동간분제량농도가가강순박대궁경암세포적억제증식병가조체세포주기G1~S기진정，해생물학효응가능여촉진p21/p53단백표체상조상관。
Objective To investigate the changes of cell proliferation and cell cycle distribution after treatment of Jin-shuixian Powder Suspension combined with Cisplatin for cervical C-33A cells on the level of cell biology and molecu-lar biology, and detect the effect on the expression of associated protein. Methods Different concentrations of Jinshuix-ian Powder Suspension(0.1, 0.2, 0.5, 1 mg/mL)were used for treatment of cervical C-33A cell, and 0.9%sodium chlo-ride solution injection was selected as the control; the proliferative activity of inhibitory rate of different concentrations of Jinshuixian 12, 24, 48 and 96 h after treatment was detected by CCK8 assay. Proliferative activity of cervical C-33A cell 48 h after the treatment of 0.9% sodium chloride solution injection, 30 μg/mL Cisplatin, best inhibition concentra-tion of Jinshuixian Powder Suspension, Cisplatin combined with best inhibition concentration of Jinshuixian Powder Suspension was detected; the changes of cell cycle distribution and expression of related proteins cyclin proteins p21 and p53 48 h after the treatment of the above treatment were detected by Western blot. Results CCK8 method showed that, 0.5 mg/mL Jinshuixian Powder Suspension had most obviously inhibit cell proliferation, and the inhibition rate reached 61.2%, compared with the 0.1 mg/mL and 0.2 mg/mL Jinshuixian Powder Suspension (19.8% v s 37.6%), the differences were statistically significant (P<0.05); the inhibition rate of 30 μg/mL Cisplatin combined with 0.5 mg/mL Jinshuixian Powder Suspension on cervical C-33A cells 48 h after treatment reached 82.05%, compared with the proliferation effect of Cisplatin alone (65.24%), the difference was statistically significant (P < 0.05);flow cytometry analysis showed, Jinshuixian Powder <br> Suspension combined with Cisplatin made cervical C-33A cells G1 arrest; the expressions of p21 and p53 48 h after treatment of Cisplatin combined with 0.5 mg/mL Jinshuixian Powder Suspension were obviously increased, compared with 0.9% sodium chloride solution injection and Cisplatin alone, the differences were statistically significant (P <0.05). Conclusion The appropriate appropriate dose concentration of Jinshuixian Powder Suspension can enhance the proliferation inhibition of Cisplatin on cervical cancer cell and induce cell cycle arrested in G1-S phase, such biological effects on cancer cells may correlated with the high expression of p21/p53 protein.