CAJ | 학술논문

目的应用非显带技术,分析人体肝癌细胞系SMMC-7721的染色体核型.方法体外培养SMMC-7721细胞,通过秋水仙素使细胞同步化,低渗、滴片、吉姆萨染色,进行非显带染色体核型分析.结果数目方面,SMMC-7721细胞系核型的众数为60~70条,峰值主要出现在E组,通过分析染色体数目在50~70个之间的较清晰的25个核型发现,E、F、G组染色体总数大多过半甚至超过60%;结构方面,分辨出双着丝粒染色体、含有3个着丝粒的染色体和环状染色体.结论上述染色体的数目异常与结构畸变,对认识肝细胞的转化、恶变以及临床的初步诊治提供了细胞遗传学基础.
목적 응용비현대기술,분석인체간암세포계SMMC-7721적염색체핵형.방법 체외배양SMMC-7721세포,통과추수선소사세포동보화,저삼、적편、길모살염색,진행비현대염색체핵형분석.결과 수목방면,SMMC-7721세포계핵형적음수위60~70조,봉치주요출현재E조,통과분석염색체수목재50~70개지간적교청석적25개핵형발현,E、F、G조염색체총수대다과반심지초과60%;결구방면,분변출쌍착사립염색체、함유3개착사립적염색체화배상염색체.결론 상술염색체적수목이상여결구기변,대인식간세포적전화、악변이급림상적초보진치제공료세포유전학기출.
Objective To analyze karyotype of human hepatoma cell lines SMMC-7721 using non-banding technique. Methods SMMC-7721 cells were cultured in vitro, followed by cell synchronization with colchicine, hy-potonic treatment and Giemsa staining, and then non-banding karyotype was analyzed. Results The majority number of karyotypes of SMMC-7721 cell lines was 60 to 70. Peaks occurred mainly in the E group, and the majority of E, F, G groups were more than half of the total number of chromosomes or even more than 60%(25/128). For the structure of chromosomes, dicentric chromosome, chromosome sharing three centromeres and ring chromosome could be found in a few cells. Conclusion The analysis of the abnormal chromosome will help us to deeply understand transforma-tion and deterioration of liver cells, which provides the genetic basis for preliminary clinical diagnosis and treatment.