海南医学
海南醫學
해남의학
Hainan Medical Journal
2015年
20期
2965-2967
,共3页
胚胎%肾%巢蛋白%免疫组织化学
胚胎%腎%巢蛋白%免疫組織化學
배태%신%소단백%면역조직화학
Embryo%Kidney%Nestin%Immunohistochemistry
目的 通过研究不同时期人胚胎肾中巢蛋白的表达,探讨巢蛋白在肾发育过程中的作用.方法 取12例3~8个月人胚胎肾组织,常规石蜡切片,采用免疫组织化学法检测巢蛋白在胚胎肾中的表达及分布.结果 3~8个月人胚胎肾组织均有巢蛋白的表达.巢蛋白阳性细胞主要分布于肾小球、肾小囊壁层和肾间质内的微血管及毛细血管壁上,且其表达量随胎龄的增加而减少,而在近端小管、远端小管的上皮中未发现阳性细胞.结论 正常人胚肾中有巢蛋白的表达,随着胎龄的增加,巢蛋白表达量下降,提示巢蛋白可能参与胚胎肾的发育.
目的 通過研究不同時期人胚胎腎中巢蛋白的錶達,探討巢蛋白在腎髮育過程中的作用.方法 取12例3~8箇月人胚胎腎組織,常規石蠟切片,採用免疫組織化學法檢測巢蛋白在胚胎腎中的錶達及分佈.結果 3~8箇月人胚胎腎組織均有巢蛋白的錶達.巢蛋白暘性細胞主要分佈于腎小毬、腎小囊壁層和腎間質內的微血管及毛細血管壁上,且其錶達量隨胎齡的增加而減少,而在近耑小管、遠耑小管的上皮中未髮現暘性細胞.結論 正常人胚腎中有巢蛋白的錶達,隨著胎齡的增加,巢蛋白錶達量下降,提示巢蛋白可能參與胚胎腎的髮育.
목적 통과연구불동시기인배태신중소단백적표체,탐토소단백재신발육과정중적작용.방법 취12례3~8개월인배태신조직,상규석사절편,채용면역조직화학법검측소단백재배태신중적표체급분포.결과 3~8개월인배태신조직균유소단백적표체.소단백양성세포주요분포우신소구、신소낭벽층화신간질내적미혈관급모세혈관벽상,차기표체량수태령적증가이감소,이재근단소관、원단소관적상피중미발현양성세포.결론 정상인배신중유소단백적표체,수착태령적증가,소단백표체량하강,제시소단백가능삼여배태신적발육.
Objective To study the role of nestin in kidney development through the expression of nestin in human embryonic kidney of different development stage. Methods Embryonic kidney tissues of 3~8 months were obtained and fixed in routine paraffin sections. Immunohistochemical assay was used to detect the expression and dis-tribution of nestin in embryonic kidney. Results Nestin was expressed in human embryonic kidneys of 3~8 months. Nestin positive cells were mainly distributed in glomerulus, renal capsule, and renal interstitial capillaries, while no positive signals could be observed in renal tubules. Expression was decreased with the aging of embryonic kidney. Conclusion Nestin was expressed in human embryonic kidney, and the expression was decreased with the develop-ing of human embryonic kidney, which indicated that nestin might play a role in kidney development.
