海南医学
海南醫學
해남의학
Hainan Medical Journal
2015年
20期
2982-2984
,共3页
儿童%原发性肾病综合征%P-糖蛋白170
兒童%原髮性腎病綜閤徵%P-糖蛋白170
인동%원발성신병종합정%P-당단백170
Children%Primary nephrotic syndrome (PNS)%P-glycoprotein 170
目的 探讨复发型原发性肾病综合征(PNS)患儿肾组织P-糖蛋白170 (P-gp170)的表达及其临床意义.方法 共选取2012年1月至2013年12月间我院住院诊治的58例PNS患儿,包括初发未用糖皮质激素(GC)组13例,复发未用GC组18例,复发用GC组27例,穿刺取得肾组织,另取12例正常肾组织作为对照组.采用免疫组化法检测并比较各组肾组织中P-gp170的表达,分析P-gp170表达与复发型PNS患儿病程、首次复发与初发间隔时间、糖皮质激素用量的关系.结果 初发未用GC组、复发未用GC组和复发用GC组三组肾组织P-gp170的表达均高于正常对照组,差异均有统计学意义(P<0.05),而复发未用GC组与初发未用GC组P-gp170的表达差异则无统计学意义(P>0.05),复发用GC组P-gp170的表达又高于复发未用GC组,差异有统计学意义(P<0.05).复发用GC组中P-gp170表达与病程、糖皮质激素用量呈正相关(r=0.651,r=0.788,P<0.05),而与首次复发与初发间隔时间呈负相关(r=-0.794,P<0.05).结论 复发PNS患儿肾组织中P-gp170高表达,其表达与PNS患儿病程、复发时间及糖皮质激素用量有关,对P-gp170进行检测可有效指导PNS患儿的临床治疗,判断预后.
目的 探討複髮型原髮性腎病綜閤徵(PNS)患兒腎組織P-糖蛋白170 (P-gp170)的錶達及其臨床意義.方法 共選取2012年1月至2013年12月間我院住院診治的58例PNS患兒,包括初髮未用糖皮質激素(GC)組13例,複髮未用GC組18例,複髮用GC組27例,穿刺取得腎組織,另取12例正常腎組織作為對照組.採用免疫組化法檢測併比較各組腎組織中P-gp170的錶達,分析P-gp170錶達與複髮型PNS患兒病程、首次複髮與初髮間隔時間、糖皮質激素用量的關繫.結果 初髮未用GC組、複髮未用GC組和複髮用GC組三組腎組織P-gp170的錶達均高于正常對照組,差異均有統計學意義(P<0.05),而複髮未用GC組與初髮未用GC組P-gp170的錶達差異則無統計學意義(P>0.05),複髮用GC組P-gp170的錶達又高于複髮未用GC組,差異有統計學意義(P<0.05).複髮用GC組中P-gp170錶達與病程、糖皮質激素用量呈正相關(r=0.651,r=0.788,P<0.05),而與首次複髮與初髮間隔時間呈負相關(r=-0.794,P<0.05).結論 複髮PNS患兒腎組織中P-gp170高錶達,其錶達與PNS患兒病程、複髮時間及糖皮質激素用量有關,對P-gp170進行檢測可有效指導PNS患兒的臨床治療,判斷預後.
목적 탐토복발형원발성신병종합정(PNS)환인신조직P-당단백170 (P-gp170)적표체급기림상의의.방법 공선취2012년1월지2013년12월간아원주원진치적58례PNS환인,포괄초발미용당피질격소(GC)조13례,복발미용GC조18례,복발용GC조27례,천자취득신조직,령취12례정상신조직작위대조조.채용면역조화법검측병비교각조신조직중P-gp170적표체,분석P-gp170표체여복발형PNS환인병정、수차복발여초발간격시간、당피질격소용량적관계.결과 초발미용GC조、복발미용GC조화복발용GC조삼조신조직P-gp170적표체균고우정상대조조,차이균유통계학의의(P<0.05),이복발미용GC조여초발미용GC조P-gp170적표체차이칙무통계학의의(P>0.05),복발용GC조P-gp170적표체우고우복발미용GC조,차이유통계학의의(P<0.05).복발용GC조중P-gp170표체여병정、당피질격소용량정정상관(r=0.651,r=0.788,P<0.05),이여수차복발여초발간격시간정부상관(r=-0.794,P<0.05).결론 복발PNS환인신조직중P-gp170고표체,기표체여PNS환인병정、복발시간급당피질격소용량유관,대P-gp170진행검측가유효지도PNS환인적림상치료,판단예후.
Objective To explore the expression and clinical significance of P-glycoprotein 170 (P-gp170) in renal tissue of children with relapsed primary nephrotic syndrome (PNS). Methods Fifty-eight children with PNS diagnosed in our hospital from January 2012 to December 2013 were selected, including 13 cases of incipient PNS without glucocorticoid (GC) treatment (incipient PNS without GC group), 18 cases of relapsed PNS without GC treat-ment (elapsed PNS without GC group), 27 cases of relapsed PNS with GC treatment (elapsed PNS with GC group), and kidney tissues were collected through puncture. Twelve cases of normal renal tissues were selected as control group. The expression of P-gp170 of renal tissues in all groups were detected by immunohistochemical method, and the correlations between P-gp170 expression and disease course, recurrence time, the dosage of glucocorticoid were analyzed. Results The expression of P-gp170 were higher in incipient PNS without GC group, relapsed PNS without GC group and relapsed PNS with GC group than control group, and the differences had statistical significance (all P<0.05). P-gp170 expression had no statistically significant difference between relapsed PNS without GC group and in-cipient PNS without GC group (all P>0.05), and P-gp170 expression were higher in relapsed PNS with GC group than relapsed PNS without GC group, with statistically significant differences (all P<0.05). P-gp170 expression were posi-tively correlated with duration, dosage of glucocorticoid in relapsed PNS with GC group (r=0.651, r=0.788, P<0.05), and was negatively correlated with recurrence time (r=0.794, P<0.05). Conclusion P-gp170 is highly expressed in renal tissue of children with relapsed PNS. Its expression is closely related with duration, recurrence time and dosage of glucocorticoid. Testing the P-gp170 can effectively guide the clinical treatment of children with PNS and judge the prognosis.
