牙体牙髓牙周病学杂志
牙體牙髓牙週病學雜誌
아체아수아주병학잡지
Chinese Journal of Conservative Dentistry
2015年
10期
577-583
,共7页
生物活性玻璃%丝素蛋白%牙髓干细胞%组织工程
生物活性玻璃%絲素蛋白%牙髓榦細胞%組織工程
생물활성파리%사소단백%아수간세포%조직공정
bioactive glass%human dental pulp stem cells%silk fibroin%tissue engineering
目的：：研究生物活性玻璃58S/丝素蛋白( BG58S/SF)膜对人牙髓干细胞( hDPSCs)增殖、分化的影响。方法：制备纯SF膜(A组)和1 mg/mL(B组)、5 mg/mL(C组)BG58S/SF膜预孵育24 h后分别接种hDPSCs。于培养4、7、14、21 d时,CCK-8法检测hDPSCs的增殖能力；扫描电镜和荧光染色观察hDPSCs在膜材料表面的粘附和增殖状态；ALP活性试验评估hDPSCs的分化潜能；Real-Time PCR检测hDPSCs向成牙本质细胞分化特异性相关基因的表达水平。结果：hDPSCs在纯SF膜和BG58S/SF膜材料上粘附良好；从第7天起,与A组相比,B、C组细胞ALP活性显著增高, hDPSCs向成牙本质细胞分化特异性相关基因表达水平上调更加显著(P<0.05),且C组均高于B组。结论：BG58S/SF膜能促进hDPSCs粘附、增殖与分化。
目的：：研究生物活性玻璃58S/絲素蛋白( BG58S/SF)膜對人牙髓榦細胞( hDPSCs)增殖、分化的影響。方法：製備純SF膜(A組)和1 mg/mL(B組)、5 mg/mL(C組)BG58S/SF膜預孵育24 h後分彆接種hDPSCs。于培養4、7、14、21 d時,CCK-8法檢測hDPSCs的增殖能力；掃描電鏡和熒光染色觀察hDPSCs在膜材料錶麵的粘附和增殖狀態；ALP活性試驗評估hDPSCs的分化潛能；Real-Time PCR檢測hDPSCs嚮成牙本質細胞分化特異性相關基因的錶達水平。結果：hDPSCs在純SF膜和BG58S/SF膜材料上粘附良好；從第7天起,與A組相比,B、C組細胞ALP活性顯著增高, hDPSCs嚮成牙本質細胞分化特異性相關基因錶達水平上調更加顯著(P<0.05),且C組均高于B組。結論：BG58S/SF膜能促進hDPSCs粘附、增殖與分化。
목적：：연구생물활성파리58S/사소단백( BG58S/SF)막대인아수간세포( hDPSCs)증식、분화적영향。방법：제비순SF막(A조)화1 mg/mL(B조)、5 mg/mL(C조)BG58S/SF막예부육24 h후분별접충hDPSCs。우배양4、7、14、21 d시,CCK-8법검측hDPSCs적증식능력；소묘전경화형광염색관찰hDPSCs재막재료표면적점부화증식상태；ALP활성시험평고hDPSCs적분화잠능；Real-Time PCR검측hDPSCs향성아본질세포분화특이성상관기인적표체수평。결과：hDPSCs재순SF막화BG58S/SF막재료상점부량호；종제7천기,여A조상비,B、C조세포ALP활성현저증고, hDPSCs향성아본질세포분화특이성상관기인표체수평상조경가현저(P<0.05),차C조균고우B조。결론：BG58S/SF막능촉진hDPSCs점부、증식여분화。
AIM:To investigate the effect of bioactive glass 58S/silk fibroin (BG58S/SF) membrane on the proliferation and differentiation of human dental pulp stem cells ( hDPSCs) . METHODS:hDPSCs were seeded on pure SF membrane and BG58S/SF with BG58S at 1 mg/mL and 5 mg/mL respectively after the materials were incubated in cell culture medium for 24 hours. Cell proliferation was assessed by CCK-8 kit after 4, 7, 14 and 21 d culture re-spectively. The adhesion of hDPSCs on the material surface was evaluated by SEM and cytoskeleton staining;ALP ac-tivity was measured by ALP kit and the expression of odontoblastic differentiation -related genes was measured by RT-PCR. One-way ANOVA and paired test were used for statistical analysis . RESULTS: hDPSCs proliferated well on the surface of the membranes throughout the culture period. ALP activity was enhanced in all the 3 groups from day 7, BG58S/SF membrane groups showed higher ALP activity than SF group (P<0. 05). BG58S/SF groups showed higher up-regulation of odontoblastic differentiation-associated genes than to SF group (P<0. 05). ALP activity and orodontoblastic differentiation-associated gene expression level in 5 mg/mL BG58S/SF group was higher than that in 1 mg/mL group (P<0. 05). CONCLUSION:BG58S/SF membrane may promote the proliferation and odontoblastic differentiation of hDPSCs.
