海南医学
海南醫學
해남의학
Hainan Medical Journal
2015年
20期
3023-3025
,共3页
卵巢癌%卵巢内异症囊肿%CDH1基因%甲基化%HE4
卵巢癌%卵巢內異癥囊腫%CDH1基因%甲基化%HE4
란소암%란소내이증낭종%CDH1기인%갑기화%HE4
Ovarian cancer%Ovarian endometriosis cyst%CDH1 gene%Methylation%HE4
目的 探讨组织上皮-钙粘连素(CDH1)基因异常甲基化和血清人附睾分泌蛋白4 (HE4)检测对卵巢癌和卵巢内异症囊肿的鉴别价值.方法 选取卵巢癌患者(卵巢癌组) 38例、卵巢内异症囊肿患者(内异症组) 41例,另选取子宫或卵巢良性病变患者42例(对照组),采用甲基化聚合酶联反应技术检测CDH1基因启动子区甲基化状态,采用酶联免疫吸附试验检测血清HE4水平.比较以上指标的变化.结果 CDH1 DNA在内异症组、卵巢癌组及对照组中的甲基化表达率分别为4.8%(2/41)、39.4%(15/38)、2.3%(1/42).内异症组的CDH1 DNA甲基化表达率与对照组比较差异无统计学意义(χ2=0.37,P=0.542),卵巢癌组的CDH1基因甲基化表达率与对照组比较差异有统计学意义(χ2=11.24,P=0.001),卵巢癌组的CDH1基因甲基化表达率与内异症组比较差异有统计学意义(χ2=13.79,P=0.000).血清HE4在内异症组、卵巢癌组及对照组水平分别为52.7 pmol/L、537.2 pmol/L、50.3 pmol/L,内异症组血清HE4水平与对照组比较差异无统计学意义(t=0.63,P=0.532),卵巢癌组的血清HE4水平与对照组比较显著增高,差异有统计学意义(t=54.27,P=0.000),卵巢癌组与内异症组比较显著增高,差异有统计学意义(t=55.33,P=0.000).卵巢癌组CDH1基因甲基化DNA表达量与血清HE4水平具有相关性(r=0.527,P<0.01),卵巢癌组CDH1甲基化DNA表达量、血清HE4水平分别与CA125水平具有相关性(r=0.539、0.520,P<0.01).内异症组血清HE4水平与CA125水平无明显相关性(r=0.127,P>0.05).结论 联合检测组织CDH1 DNA甲基化的发生和血清HE4水平可应用于卵巢内异症囊肿和卵巢癌的鉴别诊断,提高两种疾病的诊断率.
目的 探討組織上皮-鈣粘連素(CDH1)基因異常甲基化和血清人附睪分泌蛋白4 (HE4)檢測對卵巢癌和卵巢內異癥囊腫的鑒彆價值.方法 選取卵巢癌患者(卵巢癌組) 38例、卵巢內異癥囊腫患者(內異癥組) 41例,另選取子宮或卵巢良性病變患者42例(對照組),採用甲基化聚閤酶聯反應技術檢測CDH1基因啟動子區甲基化狀態,採用酶聯免疫吸附試驗檢測血清HE4水平.比較以上指標的變化.結果 CDH1 DNA在內異癥組、卵巢癌組及對照組中的甲基化錶達率分彆為4.8%(2/41)、39.4%(15/38)、2.3%(1/42).內異癥組的CDH1 DNA甲基化錶達率與對照組比較差異無統計學意義(χ2=0.37,P=0.542),卵巢癌組的CDH1基因甲基化錶達率與對照組比較差異有統計學意義(χ2=11.24,P=0.001),卵巢癌組的CDH1基因甲基化錶達率與內異癥組比較差異有統計學意義(χ2=13.79,P=0.000).血清HE4在內異癥組、卵巢癌組及對照組水平分彆為52.7 pmol/L、537.2 pmol/L、50.3 pmol/L,內異癥組血清HE4水平與對照組比較差異無統計學意義(t=0.63,P=0.532),卵巢癌組的血清HE4水平與對照組比較顯著增高,差異有統計學意義(t=54.27,P=0.000),卵巢癌組與內異癥組比較顯著增高,差異有統計學意義(t=55.33,P=0.000).卵巢癌組CDH1基因甲基化DNA錶達量與血清HE4水平具有相關性(r=0.527,P<0.01),卵巢癌組CDH1甲基化DNA錶達量、血清HE4水平分彆與CA125水平具有相關性(r=0.539、0.520,P<0.01).內異癥組血清HE4水平與CA125水平無明顯相關性(r=0.127,P>0.05).結論 聯閤檢測組織CDH1 DNA甲基化的髮生和血清HE4水平可應用于卵巢內異癥囊腫和卵巢癌的鑒彆診斷,提高兩種疾病的診斷率.
목적 탐토조직상피-개점련소(CDH1)기인이상갑기화화혈청인부고분비단백4 (HE4)검측대란소암화란소내이증낭종적감별개치.방법 선취란소암환자(란소암조) 38례、란소내이증낭종환자(내이증조) 41례,령선취자궁혹란소량성병변환자42례(대조조),채용갑기화취합매련반응기술검측CDH1기인계동자구갑기화상태,채용매련면역흡부시험검측혈청HE4수평.비교이상지표적변화.결과 CDH1 DNA재내이증조、란소암조급대조조중적갑기화표체솔분별위4.8%(2/41)、39.4%(15/38)、2.3%(1/42).내이증조적CDH1 DNA갑기화표체솔여대조조비교차이무통계학의의(χ2=0.37,P=0.542),란소암조적CDH1기인갑기화표체솔여대조조비교차이유통계학의의(χ2=11.24,P=0.001),란소암조적CDH1기인갑기화표체솔여내이증조비교차이유통계학의의(χ2=13.79,P=0.000).혈청HE4재내이증조、란소암조급대조조수평분별위52.7 pmol/L、537.2 pmol/L、50.3 pmol/L,내이증조혈청HE4수평여대조조비교차이무통계학의의(t=0.63,P=0.532),란소암조적혈청HE4수평여대조조비교현저증고,차이유통계학의의(t=54.27,P=0.000),란소암조여내이증조비교현저증고,차이유통계학의의(t=55.33,P=0.000).란소암조CDH1기인갑기화DNA표체량여혈청HE4수평구유상관성(r=0.527,P<0.01),란소암조CDH1갑기화DNA표체량、혈청HE4수평분별여CA125수평구유상관성(r=0.539、0.520,P<0.01).내이증조혈청HE4수평여CA125수평무명현상관성(r=0.127,P>0.05).결론 연합검측조직CDH1 DNA갑기화적발생화혈청HE4수평가응용우란소내이증낭종화란소암적감별진단,제고량충질병적진단솔.
Objective To investigate the abnormal methylation of CDH1 gene and the detection of serum hu-man epididymis protein 4 (HE4) in the identification of ovarian cancer and ovarian endometriosis cyst. Methods Thir-ty-eight patients with ovarian cancer (group A), 41 patients with ovarian ectopic cyst (group B) and 42 patients with be-nign uterine or ovarian lesion (the control group) were enrolled in the study. The methylation status of CDH1 gene pro-moter region was detected by methylation-specific polymerase chain reaction, and the levels of serum HE4 were de-tected with enzyme-linked immunosorbent assay. The changes of the above indicators were compared. Results The expression rates of CDH1 DNA methylation in group B, group A, control group were 4.8% (2/41), 39.4% (15/38), 2.3%(1/42), respectively, with no statistically significant difference between group B and the control group (χ2=0.37, P=0.542), but statistically significant difference between group A and the control group (χ2=11.24, P=0.001), as well as between group A and group B (χ2=13.79, P=0.000). The levels of serum HE4 in group B, group A and control group were 52.7 pmol/L, 537.2 pmol/L, 50.3 pmol/L, respectively, with no statistically significant difference between group B and the control group (t=0.63, P=0.532), but statistically significant difference between group A and the control group (t=54.27, P=0.000), as well as between group A and group B (t=55.33, P=0.000). In group A, the DNA expres-sion quantity of CDH1 gene methylation and levels of serum HE4 are correlated (r=0.527, P<0.01), which are respec-tively correlated to CA125 levels (t=0.539, 0.520, P<0.01). In group B, the levels of serum HE4 had no correlation with CA125 levels (r=0.127, P>0.05). Conclusion Joint detection of CDH1 gene methylation and serum HE4 levels can be applied to the differential diagnosis between ovarian endometriosis cyst and ovarian cancer, which improves the diagnosis rate of the two kinds of diseases.
