CAJ | 학술논문

目的：探索中国汉族人群中5－羟色胺（5－HT ）1A受体、5－HT2A受体基因多态性与伴有和不伴有抑郁发作史的阿尔茨海默病（AD ）的关系。方法采用病例对照研究方法，以130例不伴有抑郁发作史的AD患者、85例伴有抑郁发作史的AD患者和120名健康老年人为研究对象；用聚合酶链反应－限制性片段长度多态性（PCR －RFLP）技术检测5－HT1A C（－1019）G和5－HT2A T102C多态性。结果（1）伴有抑郁发作史的AD组携带5－HT1AG等位基因、G／C基因型、G／G基因型频率均高于不伴有抑郁发作史的AD组和对照组，差异有统计学意义（P ＜0．05）；不伴有抑郁发作史的AD组与对照组比较，5－HT1A等位基因及基因型频率的差异无统计学意义（P ＞0．05）。（2）伴有抑郁发作史的AD组携带5－HT2A C等位基因、C／C基因型频率均高于不伴有抑郁发作史的AD组（P ＜0．05），与对照组比较，C／C基因型频率更高（P＜0．05），等位基因及其他基因型频率的差异无统计学意义（P＞0．05）；不伴有抑郁发作史的AD患者组与对照组比较，5－HT2A等位基因及基因型频率的差异无统计学意义（P＞0．05）。结论5－HT1AC（－1019）G、5－HT2AT102C基因多态性在有抑郁发作史的AD患者中可能发挥着重要的作用，5－HT1A G等位基因、5－HT2A C等位基因可能均是其风险因子。
목적：탐색중국한족인군중5－간색알（5－HT ）1A수체、5－HT2A수체기인다태성여반유화불반유억욱발작사적아이자해묵병（AD ）적관계。방법채용병례대조연구방법，이130례불반유억욱발작사적AD환자、85례반유억욱발작사적AD환자화120명건강노년인위연구대상；용취합매련반응－한제성편단장도다태성（PCR －RFLP）기술검측5－HT1A C（－1019）G화5－HT2A T102C다태성。결과（1）반유억욱발작사적AD조휴대5－HT1AG등위기인、G／C기인형、G／G기인형빈솔균고우불반유억욱발작사적AD조화대조조，차이유통계학의의（P ＜0．05）；불반유억욱발작사적AD조여대조조비교，5－HT1A등위기인급기인형빈솔적차이무통계학의의（P ＞0．05）。（2）반유억욱발작사적AD조휴대5－HT2A C등위기인、C／C기인형빈솔균고우불반유억욱발작사적AD조（P ＜0．05），여대조조비교，C／C기인형빈솔경고（P＜0．05），등위기인급기타기인형빈솔적차이무통계학의의（P＞0．05）；불반유억욱발작사적AD환자조여대조조비교，5－HT2A등위기인급기인형빈솔적차이무통계학의의（P＞0．05）。결론5－HT1AC（－1019）G、5－HT2AT102C기인다태성재유억욱발작사적AD환자중가능발휘착중요적작용，5－HT1A G등위기인、5－HT2A C등위기인가능균시기풍험인자。
Objective To investigate the relationship between 5 -hydroxytryptamine(5 -HT )1A and 5-HT2A receptor gene polymorphisms and Alzheimer disease(AD) with or without a history of de‐pressive episodes in Han Chinese .Methods With the method of case -control study ,130 AD patients without history of depressive episodes ,85 AD patients with history of depressive episodes and 120 healthy old individuals were included .The 5-HT1A C(-1019)G and 5 -HT2A T102C polymorphisms were analyzed with the technique of polymerase chain reaction - restriction fragment length polymor‐phism(PCR-RFLP).Results (1)ThefrequenciesofalleleGandgenotypeG/C,G/Gof5-HT1Ain AD with depressive episodes were significantly higher than those in the normal control group and AD without depressive episodes (P < 0 .05) .No significant difference in frequencies of 5 -HT1A genotype and allele between AD without depressive episodes and the normal control group was found (P> 0 .05) . (2) The frequencies of allele C and genotype C/C of 5-HT2A in AD with depressive episodes were sig‐nificantly higher than those in AD without depressive episodes (P < 0 .05) .Compared with normal group ,the frequency of 5-HT2A C/C genotype was higher in AD with depressive episodes (P < 0 .05) and there was no statistical significance in alleles and other genotypes of 5 -HT2A (P > 0 .05) .There was no significant difference in 5 -HT2A allele and genotype frequency between AD without depressive episodesandthenormalcontrolgroup(P>0.05).Conclusions 5-HT1AC(-1019)G,5-HT2A T102C gene polymorphisms may play an important role in AD patients with depressive episodes .5 -HT1A allele G and 5-HT2A allele C may be the risk factors .