药学研究
藥學研究
약학연구
Journal of Pharmaceutical Research
2015年
11期
642-644
,共3页
五酯胶囊%五味子醇甲%五味子酯甲%五味子甲素%五味子乙素%高效液相色谱法
五酯膠囊%五味子醇甲%五味子酯甲%五味子甲素%五味子乙素%高效液相色譜法
오지효낭%오미자순갑%오미자지갑%오미자갑소%오미자을소%고효액상색보법
Wuzhi capsule%Schisandrin%Schisantherin A%Schizandrin A%Schizandrin B%HPLC
目的：高效液相色谱法测定五酯胶囊中五味子醇甲、五味子酯甲、五味子甲素和五味子乙素的含量。方法采用 Agilent Zorbax SB - C18色谱柱（4.6 mm ×250 mm，5μm），乙腈-0.1%磷酸水溶液梯度洗脱，柱温为40℃，变换波长法。结果五味子醇甲、五味子酯甲、五味子甲素和五味子乙素分别在0.01055~2.110μg、0.01211~2.422μg、0.01345~2.690μg、0.00934~1.868μg 范围内呈现良好的线性关系，平均回收率分别为99.36%、99.53%、99.23%、99.78%，RSD 均小于2.0%。结论该方法准确，灵敏度高，重复性好，为五酯胶囊的质量评价提供参考。
目的：高效液相色譜法測定五酯膠囊中五味子醇甲、五味子酯甲、五味子甲素和五味子乙素的含量。方法採用 Agilent Zorbax SB - C18色譜柱（4.6 mm ×250 mm，5μm），乙腈-0.1%燐痠水溶液梯度洗脫，柱溫為40℃，變換波長法。結果五味子醇甲、五味子酯甲、五味子甲素和五味子乙素分彆在0.01055~2.110μg、0.01211~2.422μg、0.01345~2.690μg、0.00934~1.868μg 範圍內呈現良好的線性關繫，平均迴收率分彆為99.36%、99.53%、99.23%、99.78%，RSD 均小于2.0%。結論該方法準確，靈敏度高，重複性好，為五酯膠囊的質量評價提供參攷。
목적：고효액상색보법측정오지효낭중오미자순갑、오미자지갑、오미자갑소화오미자을소적함량。방법채용 Agilent Zorbax SB - C18색보주（4.6 mm ×250 mm，5μm），을정-0.1%린산수용액제도세탈，주온위40℃，변환파장법。결과오미자순갑、오미자지갑、오미자갑소화오미자을소분별재0.01055~2.110μg、0.01211~2.422μg、0.01345~2.690μg、0.00934~1.868μg 범위내정현량호적선성관계，평균회수솔분별위99.36%、99.53%、99.23%、99.78%，RSD 균소우2.0%。결론해방법준학，령민도고，중복성호，위오지효낭적질량평개제공삼고。
Objective To determine the contents of schisandrin,schisantherin A,schizandrin A and schizandrin B in Wuzhi Capsules by HPLC. Methods Agilent Zorbax SB - C18(4. 6 mm × 250 mm,5 μm)was adopted with acetonitrile -0. 1% phosphoric acid as the mobile phases by gradient elution. The column temperature was 40 ℃ . The detection wave-length was changed. Results The linearity ranges of schisandrin,schisantherin A,schizandrin A and schizandrin B were 0. 010 55 ~ 2. 110 μg,0. 012 11 ~ 2. 422 μg,0. 013 45 ~ 2. 690 μg,0. 009 34 ~ 1. 868 μg,respectively. The average recov-eries were 99. 36% ,99. 53% ,99. 23% ,99. 78% ,respectively,and the RSDs were less than 2. 0% . Conclusion The method was accurate,sensitive,reproducible,it can be used for the quality control of Wuzhi Capsules.
