药学研究
藥學研究
약학연구
Journal of Pharmaceutical Research
2015年
11期
640-641
,共2页
更年安胶囊%高效液相色谱法%含量
更年安膠囊%高效液相色譜法%含量
경년안효낭%고효액상색보법%함량
Gengnian′an Capsules%HPLC%Content
目的:建立更年安胶囊含量的高效液相色谱测定方法。方法采用大连依利特 C18色谱柱,流动相为乙腈-水-冰醋酸(60:40:1),流速:1.0 mL·min -1,检测波长437 nm,进样量10μL。结果在该色谱条件下,在浓度为0.040~0.600 mg·mL -1范围内线性关系良好,r =0.9939,重复性试验 RSD 为1.35%,平均回收率为96.67%(RSD =1.33%)。结论本法灵敏、简便、准确、重复性好,能有效控制更年安胶囊的含量。
目的:建立更年安膠囊含量的高效液相色譜測定方法。方法採用大連依利特 C18色譜柱,流動相為乙腈-水-冰醋痠(60:40:1),流速:1.0 mL·min -1,檢測波長437 nm,進樣量10μL。結果在該色譜條件下,在濃度為0.040~0.600 mg·mL -1範圍內線性關繫良好,r =0.9939,重複性試驗 RSD 為1.35%,平均迴收率為96.67%(RSD =1.33%)。結論本法靈敏、簡便、準確、重複性好,能有效控製更年安膠囊的含量。
목적:건립경년안효낭함량적고효액상색보측정방법。방법채용대련의리특 C18색보주,류동상위을정-수-빙작산(60:40:1),류속:1.0 mL·min -1,검측파장437 nm,진양량10μL。결과재해색보조건하,재농도위0.040~0.600 mg·mL -1범위내선성관계량호,r =0.9939,중복성시험 RSD 위1.35%,평균회수솔위96.67%(RSD =1.33%)。결론본법령민、간편、준학、중복성호,능유효공제경년안효낭적함량。
Objective To establish an HPLC method for the determination of Gengnian′an Capsules. Methods Using the Dalian Elite C18 column,acetonitrile,water and glacial acetic acid(60:40:1),the flow rate was 1. 0 mL·min - 1 ,the de-tection wavelength was 437 nm and the injection volume was 10 μL. Results The linear range was good within the concen-tration of 0. 040 ~ 0. 600 mg·mL - 1 ,r = 0. 993 9,RSD of the reproducibility test was 1. 35% ,the average recovery was 96. 67%(RSD = 1. 33% ). Conclusion This method was sensitive,simple,accurate and reproducible,and could effectively control the content of Gengnian′an Capsules.