CAJ | 학술논문

研究了消肿止痛合剂对大鼠骨折修复过程中骨痂组织中血管内皮生长因子（ VEGF）表达的影响作用。选取健康SD大鼠30只，周龄5～6周，进行编号后采用随机数字表法分为消肿止痛活剂组、模型组各15只，两组SD大鼠制作股骨（单侧）闭合骨折模型，消肿止痛合剂组给予消肿止痛合剂处理（次／12 h），模型组不予以处理，两组大鼠的健侧股骨作为空白组，比较各组大鼠骨痂组织周围新生血管数量、骨痂组织中VEGF的含量变化情况。第2日、第4日消肿止痛合剂组和模型组大鼠的骨痂组织切片中，未见新生血管形成，第7日，两组大鼠骨痂组织中均有新生血管形成，且第10日数量均较第7日显著增加，第21日两组新生血管数量明显减少；第7日、第10日、第21日消肿止痛活剂组大鼠骨痂组织中新生血管数量均显著的高于模型组大鼠（P＜0．05）。第2日、第4日、第21日消肿止痛合剂组和模型组大鼠的骨痂组织切片中VEGF阳性细胞数量差异无统计学意义（P＞0．05），第7日，第10日消肿止痛活剂组大鼠骨痂组织中VEGF着色阳性细胞数量均显著高于模型组大鼠（P＜0．05）。消肿止痛合剂有利于促进大鼠骨折修复过程中骨痂组织中新生血管形成、VEGF表达，这可能与促进大鼠骨折愈合有一定的关系。
연구료소종지통합제대대서골절수복과정중골가조직중혈관내피생장인자（ VEGF）표체적영향작용。선취건강SD대서30지，주령5～6주，진행편호후채용수궤수자표법분위소종지통활제조、모형조각15지，량조SD대서제작고골（단측）폐합골절모형，소종지통합제조급여소종지통합제처리（차／12 h），모형조불여이처리，량조대서적건측고골작위공백조，비교각조대서골가조직주위신생혈관수량、골가조직중VEGF적함량변화정황。제2일、제4일소종지통합제조화모형조대서적골가조직절편중，미견신생혈관형성，제7일，량조대서골가조직중균유신생혈관형성，차제10일수량균교제7일현저증가，제21일량조신생혈관수량명현감소；제7일、제10일、제21일소종지통활제조대서골가조직중신생혈관수량균현저적고우모형조대서（P＜0．05）。제2일、제4일、제21일소종지통합제조화모형조대서적골가조직절편중VEGF양성세포수량차이무통계학의의（P＞0．05），제7일，제10일소종지통활제조대서골가조직중VEGF착색양성세포수량균현저고우모형조대서（P＜0．05）。소종지통합제유리우촉진대서골절수복과정중골가조직중신생혈관형성、VEGF표체，저가능여촉진대서골절유합유일정적관계。
Xiaozhongzhitong mixture on rat fracture healing callus tissue of vascular endothelial growth factor (VEGF) expression effect was Studied .30 healthy SD rats were selected , age 5~6 weeks after the numbers were randomly divided into model group , pain swelling group with 15 rats in each group, two groups of SD rats (Dan Ce) of femur closed fracture model , acetanilide detumescence agent group was treated with Xiaozhongzhitong agent treat -ment (12 h/times), model group received treatment .The two groups of rats femur as the blank group , the content of VEGF changes of callus were compared between each group number , neovascularization in callus .2 d, 4 d, swelling and pain of live group and the model group rats callus tissues , Were no neovascularization .7 d, are the formation of new blood vessels in rats of two groups of bony callus , in the number of 10 d were lower in 7 d significantly increased in the 21 d group , the number of new vessels were found in two the decrease in 7、10 and 21 d, the number of new vessels swelling analgesic agent group rats callus tissues were significantly higher than that in the model group rats ( P<0.05).The 2 d, 4 d, 21 d number of VEGF live pain swelling agent group and the model group rats bone tissue sections in positive cells don ’t have significant difference (P>0.05), 7 d, 10 d the number of VEGF positive stai-ning cell swelling analgesic agent group rats callus tissues were significantly higher in the model groups of rats ( P<0.05).Xiaozhongzhitong mixture is conducive to promoting the process of callus formation of blood vessels in rats fracture repair , VEGF expression , which may have some relationship with the promotion of fracture healing in rats .