CAJ | 학술논문

以人外周血为研究对象,研究照射后 RPS27L 基因的时间效应和剂量效应以及本底水平,探究其作为辐射损伤早期生物标志物的可行性.采集 3 名健康成人外周血,利用 γ射线对其进行照射至吸收剂量分别为0、0.5、1、2、4、6和10 Gy,受照后37℃培养2、4、8、12和24 h,利用实时荧光定量PCR检测RPS27L基因的表达变化,并分析在37个健康人个体中的本底水平.结果显示,受照后的4、8、12和24 h RPS27L基因表达变化显著,而且具有很好的剂量依赖关系;在不同年龄和性别的个体中 RPS27L 本底水平相对稳定.因此,RPS27L基因具有作为新的辐射损伤标志物的潜力.
이인외주혈위연구대상,연구조사후 RPS27L 기인적시간효응화제량효응이급본저수평,탐구기작위복사손상조기생물표지물적가행성.채집 3 명건강성인외주혈,이용 γ사선대기진행조사지흡수제량분별위0、0.5、1、2、4、6화10 Gy,수조후37℃배양2、4、8、12화24 h,이용실시형광정량PCR검측RPS27L기인적표체변화,병분석재37개건강인개체중적본저수평.결과현시,수조후적4、8、12화24 h RPS27L기인표체변화현저,이차구유흔호적제량의뢰관계;재불동년령화성별적개체중 RPS27L 본저수평상대은정.인차,RPS27L기인구유작위신적복사손상표지물적잠력.
In order to explore RPS27L's possibility as a biomarker of radiation damage, we studied its time-response and dose-response after irradiation as well as its background level. Three healthy adults' peripheral blood was exposed to gamma-rays with 0, 0.5, 1, 2, 4, 6, and 10 Gy, separately and cultured for 2, 4, 8, 12, and 24 h respectively. RPS27L's expression change was detected by quantitative real-time PCR. We also analyzed its background level in 37 individuals. The results showed that RPS27L had a significant difference at 4, 8, 12, 24 h and a great dose-independent response. There was also a uniform background level of RPS27L among different age and gender. Thus, RPS27L is of great potential to be a new biomarker of radiation.