CAJ | 학술논문

为有效防治烟草仓储害虫,进行了新型植物防护剂的开发研究.通过熏蒸和触杀试验测试了侧柏叶挥发油及单体成分活性,并通过GC/MS分析了侧柏叶挥发油的化学成分.结果显示:①GC/MS分析共鉴定出侧柏叶挥发油成分34种,占总成分的93.28%,其主要成分为:α-蒎烯(15.08%)、雪松醇(12.63%)、3-蒈烯(9.68%)和石竹烯(9.02%).②通过生物活性筛选,侧柏叶挥发油对烟草甲和赤拟谷盗触杀的半数致死量分别为50.45和48.59 μg/头;对二者熏蒸活性的半数致死浓度为145.40和39.25 mg/L.③从侧柏叶挥发油中得到3种活性成分:α-蒎烯、β-水芹烯和4-萜烯醇.因此,侧柏叶挥发油及其活性单体对烟草甲和赤拟谷盗均具有一定的触杀和熏蒸活性.
위유효방치연초창저해충,진행료신형식물방호제적개발연구.통과훈증화촉살시험측시료측백협휘발유급단체성분활성,병통과GC/MS분석료측백협휘발유적화학성분.결과현시:①GC/MS분석공감정출측백협휘발유성분34충,점총성분적93.28%,기주요성분위:α-파희(15.08%)、설송순(12.63%)、3-개희(9.68%)화석죽희(9.02%).②통과생물활성사선,측백협휘발유대연초갑화적의곡도촉살적반수치사량분별위50.45화48.59 μg/두;대이자훈증활성적반수치사농도위145.40화39.25 mg/L.③종측백협휘발유중득도3충활성성분:α-파희、β-수근희화4-첩희순.인차,측백협휘발유급기활성단체대연초갑화적의곡도균구유일정적촉살화훈증활성.
In order to effectively control the pests in stored tobacco leaves, new botanical protectants were studied and developed. The activities of essential oil and isolated components of Platycladus orientalis were tested via fumigation and contact toxicity assays, and the chemical components in the essential oil were analyzed by GC/MS. The results showed that:1) A total of 34 components were identified in the essential oil, which accounted for 93.28% of total components; and the major components were α-pinene (15.08%), cedrol (12.63%), 3-carene (9.68%) and caryophyllene (9.02%). 2) By means of bioactivity screening, the essential oil possessed potential contact toxicity against Lasioderma serricorne (LD50=50.45 μg/adult) and Tribolium castaneum (LD50 = 48.59 μg/adult), and it also possessed fumigation toxicity against Lasioderma serricorne(LC50=145.40 mg/L)and Tribolium castaneum(LC50=39.25 mg/L). 3) Three active components, α-pinene, β-phellandrene and 4-terpinenol, were obtained from the essential oil. It was proved that the essential oil of Platycladus orientalis and its active isolated components possessed certain contact and fumigation toxic activities against Lasioderma serricorne and Tribolium castaneum.