辐射研究与辐射工艺学报
輻射研究與輻射工藝學報
복사연구여복사공예학보
Journal of Radiation Research and Radiation Processing
2015年
5期
15-19
,共5页
马洪鸽%史盼影%林温文%张保国
馬洪鴿%史盼影%林溫文%張保國
마홍합%사반영%림온문%장보국
羧基荧光素-寡核苷酸-羧基四甲基罗丹明荧光探针%荧光强度%吸收剂量
羧基熒光素-寡覈苷痠-羧基四甲基囉丹明熒光探針%熒光彊度%吸收劑量
최기형광소-과핵감산-최기사갑기라단명형광탐침%형광강도%흡수제량
Carboxyfluorescein-oligonucleotide-carboxytetramethylrhodamine fluorescence probe%Fluorescence intensity%Absorbed dose
用 X-射线照射不同浓度羧基荧光素-寡核苷酸-羧基四甲基罗丹明(Carboxyfluorescein-Oligonucleotide- carboxytetramethylrhodamine, FAM-DNA-TAMRA)水溶液,测量溶液发出的荧光强度,研究吸收剂量与荧光强度之间的关系.结果表明,对于含不同浓度FAM-DNA-TAMRA荧光探针的水溶液,在0?20 Gy剂量范围内,荧光强度与吸收剂量之间的线性关系很好,并且荧光稳定性良好,但存在荧光本底比较高,影响测量吸收剂量灵敏度的问题.研究结果提示,FAM-DNA-TAMRA荧光探针可以用于测量水溶液的吸收剂量.
用 X-射線照射不同濃度羧基熒光素-寡覈苷痠-羧基四甲基囉丹明(Carboxyfluorescein-Oligonucleotide- carboxytetramethylrhodamine, FAM-DNA-TAMRA)水溶液,測量溶液髮齣的熒光彊度,研究吸收劑量與熒光彊度之間的關繫.結果錶明,對于含不同濃度FAM-DNA-TAMRA熒光探針的水溶液,在0?20 Gy劑量範圍內,熒光彊度與吸收劑量之間的線性關繫很好,併且熒光穩定性良好,但存在熒光本底比較高,影響測量吸收劑量靈敏度的問題.研究結果提示,FAM-DNA-TAMRA熒光探針可以用于測量水溶液的吸收劑量.
용 X-사선조사불동농도최기형광소-과핵감산-최기사갑기라단명(Carboxyfluorescein-Oligonucleotide- carboxytetramethylrhodamine, FAM-DNA-TAMRA)수용액,측량용액발출적형광강도,연구흡수제량여형광강도지간적관계.결과표명,대우함불동농도FAM-DNA-TAMRA형광탐침적수용액,재0?20 Gy제량범위내,형광강도여흡수제량지간적선성관계흔호,병차형광은정성량호,단존재형광본저비교고,영향측량흡수제량령민도적문제.연구결과제시,FAM-DNA-TAMRA형광탐침가이용우측량수용액적흡수제량.
To explore the relationship between absorbed dose and fluorescence intensity, FAM-DNA-TAMRA (Carboxyfluorescein-oligonucleotide-carboxytetramethylrhodamine) aqueous solution of different concentrations combined with X-ray irradiation was used to measure the fluorescence intensity. The results showed that the linear relation between fluorescence intensity and absorbed dose was good for different-concentration FAM-DNA-TAMRA aqueous solution containing fluorescent probe in the dose range of 0?20 Gy. The fluorescence had good stability, but the fluorescence background affected the sensitivity of absorbed dose greatly. The results suggest that the FAM-DNA-TAMRA fluorescent probe can be used to measure the absorbed dose of aqueous solution.
