CAJ | 학술논문

探讨白藜芦醇对间充质干细胞(Mesenchymal stem cells, MSCs)电离辐射诱导的炎症因子IL-6的影响,考察白藜芦醇的辐射防护作用.采用不同剂量的电离辐射处理细胞,使用ELISA试剂盒检测IL-6分泌水平,筛选出最适受照剂量4 Gy.用Western-blot和RT-PCR检测辐照后不同时间点IL-6的表达情况,筛选出最适时间点12 h.用不同浓度的白藜芦醇处理MSCs细胞,4 Gy受照后12 h检测IL-6在胞内胞外表达分泌情况.结果表明,电离辐射会引起MSCs的IL-6表达升高,白藜芦醇可以抑制电离辐射诱导IL-6的表达,提示白藜芦醇可以通过抑制电离辐射诱导的炎症因子IL-6的表达发挥辐射防护作用.
탐토백려호순대간충질간세포(Mesenchymal stem cells, MSCs)전리복사유도적염증인자IL-6적영향,고찰백려호순적복사방호작용.채용불동제량적전리복사처리세포,사용ELISA시제합검측IL-6분비수평,사선출최괄수조제량4 Gy.용Western-blot화RT-PCR검측복조후불동시간점IL-6적표체정황,사선출최괄시간점12 h.용불동농도적백려호순처리MSCs세포,4 Gy수조후12 h검측IL-6재포내포외표체분비정황.결과표명,전리복사회인기MSCs적IL-6표체승고,백려호순가이억제전리복사유도IL-6적표체,제시백려호순가이통과억제전리복사유도적염증인자IL-6적표체발휘복사방호작용.
We aim to investigate the effect of resveratrol on inflammatory factor IL-6 produced in irradiated mesenchymal stem cells (MSCs) and explore the radioprotective effect of resveratrol. MSCs were treated with different concentrations of resveratrol before irradiation, and the expression of IL-6 was detected by ELISA, Western-blot, and RT-PCR. The results showed that ionizing radiation can lead to the increasing of IL-6 expression in MSCs, and resveratrol can inhibit the expression of ionizing radiation-induced IL-6. Resveratrol can inhibit the ionizing radiation-induced expression of inflammatory cytokines IL-6, which suggests that Sirt1 may play a key role in the process of inflammation induced by irradiation.