CAJ | 학술논문

采用中能12C6+离子束辐照诱变谷氨酸生产菌谷氨酸棒杆菌,结合 3种选择性培养基初筛突变菌株,通过摇瓶复筛谷氨酸高产菌株,并进行批次发酵实验.结果表明,经 80 MeV/u碳离子束辐照后,受照菌存活率发生显著变化,通过选择性平板初筛、摇瓶复筛选育出 1株高产菌株 GM006,菌体生长和发酵有较大改进,其发酵最高产酸达 121.10 g/L,比出发菌株提高了 10.09%,糖酸转化率达60.55%,比原始菌株提高 10.09%.该 GM006突变株是很有前景的谷氨酸工业生产菌,同时证明重离子束是一种高效的辐射诱变源.
채용중능12C6+리자속복조유변곡안산생산균곡안산봉간균,결합 3충선택성배양기초사돌변균주,통과요병복사곡안산고산균주,병진행비차발효실험.결과표명,경 80 MeV/u탄리자속복조후,수조균존활솔발생현저변화,통과선택성평판초사、요병복사선육출 1주고산균주 GM006,균체생장화발효유교대개진,기발효최고산산체 121.10 g/L,비출발균주제고료 10.09%,당산전화솔체60.55%,비원시균주제고 10.09%.해 GM006돌변주시흔유전경적곡안산공업생산균,동시증명중리자속시일충고효적복사유변원.
12C6+ ion beam was used to irradiate glutamic acid-producing strains, and the strains producing higher glutamate were screened by three selective breeding mediums, and then the mutants were fermented by shake flask experiments. Finally the mutants were measured by several fermentation experiments. The results showed that the survival rate ofcorynebacterium glutamicum changed significantly after irradiation with 80 MeV/u12C6+ ion beam, and a high-yield strain GM006 was finally obtained through the two screening steps of selecting plate and shaking flask fermentation. The growth rate and glutamic acid yielding of GM006 were remarkably improved. The conversion rate of glucose was 60.55%, which increased by 10.09% compared with the original strain. The highest content of glutamic acid reached 121.10 g/L, and increased by 10.09 % compared with the original strain. This mutant will have broad application prospect as a glutamic acid producing strain, and it is also proved that the heavy ions beam is an efficient radiation resource for microorganism breeding.