中华实验眼科杂志
中華實驗眼科雜誌
중화실험안과잡지
Chinese Journal of Experimental Ophthalmology
2015年
10期
919-923
,共5页
乔军波%刘秋雨%李金%马玉春%董长宪
喬軍波%劉鞦雨%李金%馬玉春%董長憲
교군파%류추우%리금%마옥춘%동장헌
眼部静脉畸形%半乳糖凝集素-3%人%免疫组织化学%原位杂交技术
眼部靜脈畸形%半乳糖凝集素-3%人%免疫組織化學%原位雜交技術
안부정맥기형%반유당응집소-3%인%면역조직화학%원위잡교기술
Venous malformation,ocular%Galectin-3%Humans%Immunochemistry%In situ hybridization
背景 眼部静脉血管畸形呈终生渐进性发展,并表现出局部侵袭的恶性生物学行为,对局部组织器官造成形态及功能损害.研究表明,半乳糖凝集素-3(Gal-3)与恶性肿瘤的局部侵袭密切相关,研究其在眼部静脉畸形局部侵袭中的作用对治疗的选择具有重要意义.目的 研究Gal-3蛋白和mRNA在眼部静脉畸形组织中的表达及其与临床特征的关联性.方法 收集2009年6月至2014年6月在河南省人民医院血管瘤外科和河南省眼科研究所手术切除的118例眼部静脉畸形病理标本进行检测,采用免疫组织化学染色法及原位杂交方法检测眼部静脉畸形组织中Gal-3蛋白及其mRNA的表达,以20例经病理组织学证实正常的病理标本远端切缘作为对照.将患者分为Gal-3蛋白和mRNA阳性表达组和阴性表达组,比较2个组间患者病变组织中Gal-3表达与病变侵袭深度及病变形状和边界清晰度的关联.结果 常规组织病理学检查表明,眼部静脉血管畸形组织中含有多数中到大管径的血管腔隙,内衬扁平内皮细胞,免疫组织化学染色法及原位杂交方法检测显示Gal-3蛋白及其mRNA均表达于细胞质及细胞核,呈棕褐色染色.眼部静脉畸形组织中Gal-3蛋白阳性表达率为55.93％(66/118),明显高于远端手术切缘正常组织的15.00％ (3/20),差异有统计学意义(x2 =11.461,P＜0.05).眼部静脉畸形组织中Gal-3 mRNA的阳性表达率为59.32％ (70/118),明显高于远端手术切缘正常组织的20.00％(4/20),差异有统计学意义(x2=10.633,P＜0.05).≤12岁与＞12岁患者之间及不同性别间Gal-3蛋白和mRNA的阳性表达率均无统计学意义(年龄x2=0.334、0.128,均P＞0.05;性别:x2 =0.606、1.155,均P＞0.05);Gal-3蛋白及mRNA阳性表达组累及跟肌等深部组织的病变发生率明显高于Gal-3蛋白及mRNA阴性表达组,差异均有统计学意义(x2=32.688、23.695,均P＜0.05);Gal-3蛋白及mRNA阴性表达组病变呈球形且边界清楚者分别占96.15％和97.92％,Gal-3蛋白及其mRNA阳性表达组呈筛孔状且边界不清楚的病变率明显高于Gal-3蛋白及其mRNA阴性表达组,差异均有统计学意义(x2=28.255、28.186,均P＜0.05).结论 有深层组织侵袭的眼部静脉畸形组织患者病变组织中Gal-3的阳性表达率升高,Gal-3表达阳性的眼部静脉畸形组织边界不清,提示眼部静脉畸形患者病变的局部侵袭破坏与Gal-3表达上调有关.
揹景 眼部靜脈血管畸形呈終生漸進性髮展,併錶現齣跼部侵襲的噁性生物學行為,對跼部組織器官造成形態及功能損害.研究錶明,半乳糖凝集素-3(Gal-3)與噁性腫瘤的跼部侵襲密切相關,研究其在眼部靜脈畸形跼部侵襲中的作用對治療的選擇具有重要意義.目的 研究Gal-3蛋白和mRNA在眼部靜脈畸形組織中的錶達及其與臨床特徵的關聯性.方法 收集2009年6月至2014年6月在河南省人民醫院血管瘤外科和河南省眼科研究所手術切除的118例眼部靜脈畸形病理標本進行檢測,採用免疫組織化學染色法及原位雜交方法檢測眼部靜脈畸形組織中Gal-3蛋白及其mRNA的錶達,以20例經病理組織學證實正常的病理標本遠耑切緣作為對照.將患者分為Gal-3蛋白和mRNA暘性錶達組和陰性錶達組,比較2箇組間患者病變組織中Gal-3錶達與病變侵襲深度及病變形狀和邊界清晰度的關聯.結果 常規組織病理學檢查錶明,眼部靜脈血管畸形組織中含有多數中到大管徑的血管腔隙,內襯扁平內皮細胞,免疫組織化學染色法及原位雜交方法檢測顯示Gal-3蛋白及其mRNA均錶達于細胞質及細胞覈,呈棕褐色染色.眼部靜脈畸形組織中Gal-3蛋白暘性錶達率為55.93％(66/118),明顯高于遠耑手術切緣正常組織的15.00％ (3/20),差異有統計學意義(x2 =11.461,P＜0.05).眼部靜脈畸形組織中Gal-3 mRNA的暘性錶達率為59.32％ (70/118),明顯高于遠耑手術切緣正常組織的20.00％(4/20),差異有統計學意義(x2=10.633,P＜0.05).≤12歲與＞12歲患者之間及不同性彆間Gal-3蛋白和mRNA的暘性錶達率均無統計學意義(年齡x2=0.334、0.128,均P＞0.05;性彆:x2 =0.606、1.155,均P＞0.05);Gal-3蛋白及mRNA暘性錶達組纍及跟肌等深部組織的病變髮生率明顯高于Gal-3蛋白及mRNA陰性錶達組,差異均有統計學意義(x2=32.688、23.695,均P＜0.05);Gal-3蛋白及mRNA陰性錶達組病變呈毬形且邊界清楚者分彆佔96.15％和97.92％,Gal-3蛋白及其mRNA暘性錶達組呈篩孔狀且邊界不清楚的病變率明顯高于Gal-3蛋白及其mRNA陰性錶達組,差異均有統計學意義(x2=28.255、28.186,均P＜0.05).結論 有深層組織侵襲的眼部靜脈畸形組織患者病變組織中Gal-3的暘性錶達率升高,Gal-3錶達暘性的眼部靜脈畸形組織邊界不清,提示眼部靜脈畸形患者病變的跼部侵襲破壞與Gal-3錶達上調有關.
배경 안부정맥혈관기형정종생점진성발전,병표현출국부침습적악성생물학행위,대국부조직기관조성형태급공능손해.연구표명,반유당응집소-3(Gal-3)여악성종류적국부침습밀절상관,연구기재안부정맥기형국부침습중적작용대치료적선택구유중요의의.목적 연구Gal-3단백화mRNA재안부정맥기형조직중적표체급기여림상특정적관련성.방법 수집2009년6월지2014년6월재하남성인민의원혈관류외과화하남성안과연구소수술절제적118례안부정맥기형병리표본진행검측,채용면역조직화학염색법급원위잡교방법검측안부정맥기형조직중Gal-3단백급기mRNA적표체,이20례경병리조직학증실정상적병리표본원단절연작위대조.장환자분위Gal-3단백화mRNA양성표체조화음성표체조,비교2개조간환자병변조직중Gal-3표체여병변침습심도급병변형상화변계청석도적관련.결과 상규조직병이학검사표명,안부정맥혈관기형조직중함유다수중도대관경적혈관강극,내츤편평내피세포,면역조직화학염색법급원위잡교방법검측현시Gal-3단백급기mRNA균표체우세포질급세포핵,정종갈색염색.안부정맥기형조직중Gal-3단백양성표체솔위55.93％(66/118),명현고우원단수술절연정상조직적15.00％ (3/20),차이유통계학의의(x2 =11.461,P＜0.05).안부정맥기형조직중Gal-3 mRNA적양성표체솔위59.32％ (70/118),명현고우원단수술절연정상조직적20.00％(4/20),차이유통계학의의(x2=10.633,P＜0.05).≤12세여＞12세환자지간급불동성별간Gal-3단백화mRNA적양성표체솔균무통계학의의(년령x2=0.334、0.128,균P＞0.05;성별:x2 =0.606、1.155,균P＞0.05);Gal-3단백급mRNA양성표체조루급근기등심부조직적병변발생솔명현고우Gal-3단백급mRNA음성표체조,차이균유통계학의의(x2=32.688、23.695,균P＜0.05);Gal-3단백급mRNA음성표체조병변정구형차변계청초자분별점96.15％화97.92％,Gal-3단백급기mRNA양성표체조정사공상차변계불청초적병변솔명현고우Gal-3단백급기mRNA음성표체조,차이균유통계학의의(x2=28.255、28.186,균P＜0.05).결론 유심층조직침습적안부정맥기형조직환자병변조직중Gal-3적양성표체솔승고,Gal-3표체양성적안부정맥기형조직변계불청,제시안부정맥기형환자병변적국부침습파배여Gal-3표체상조유관.
Background Venous malformation damages the local tissue severely because of the progressive development and often presents with invasive biological behavior.Galectin-3 (Gal-3) is proved to be closely associated with local invasion of malignant tumor.Studying the role of Gal-3 on tissue invasion in venous malformation of ocular region is of important clinical significance.Objective This study was to explore the role of Gal-3 protein and mRNA expression in venous malformation of ocular region.Methods One hundred and eighteen pathological sections were collected from ocular venous malformation patients who received surgery in Department of Hemangioma Surgery,People's Hospital of Henan Province and Henan Eye Institute from June 2009 to June 2014.The specimens were further diagnosed by histopathological examination.Then the expressions of Gal-3 protein and mRNA in venous malformation of ocular region were detected by using immunohistochemistry and in situ hybridization and compared with 20 pieces of distal cutting edge specimens which were evidently normal.The associations of Gal-3 positive expressions with invasion and configuration of lesions were analyzed.Results Pathological examination showed that venous malformations tissues contain many big blood vessels lacuna, lined with fiat endothelial cells.Immunochemistry and in situ hybridization exhibited that Gal-3 protein and mRNA were expressed in the cytoplasm and nuclei.The positive expression rates of Gal-3 protein and mRNA in the venous malformation tissues were 55.93％ (66/118) and 59.32％ (70/118) , but those in the normal tissue were 15.00％ (3/20) and 20.00％ (4/20) ,showing significant differences between them (x2 =11.461, 10.633, both at P＜0.05).No significant differences were seen in the positive expression rates of Gal-3 protein and mRNA between the patients aged ≤ 12 years and ＞12 years or different genders (age: x2 =0.334,0.128;both at P＞0.05.gender:x2 =0.606,1.155;both at P ＞0.05).The incidence rate of invading ocular deep tissues was significantly higher in the Gal-3-positive groups than that in the Gal-3-negative groups of protein and mRNA (protein :x2 =32.688, P＜0.05;mRNA : x2 =23.695, P＜0.05).In the Gal-3-negative groups,96.15％ (Gal-3 protein negative group) and 97.92％ (Gal-3 mRNA negative group) lesions showed the spherical shape with clear boundaries.The lesions texture with the fuzzy boundaries and the incidences of vague structure in lesions were significantly higher in the Gal-3-positive groups than that in the Gal-3-negative groups of protein and mRNA (protein :x2 =28.255, P＜0.05;mRNA : 28.186, P＜0.05).Conclusions Gal-3 expression rate is raised in the deep tissue-invaded and texture disorder ocular venous malformation.These results suggest that invasion and damage of ocular venous malformation are associated with the up-regulation of Gal-3.
