CAJ | 학술논문

血友病是由于人体内缺乏相应凝血因子造成的凝血障碍，目前主要利用基因重组技术在不同细胞中表达重组凝血因子Ⅶ（Ⅶ因子）从而对血友患者出血进行治疗。为有效提高重组Ⅶ因子的产量及活性，已有研究者尝试利用不同真核表达系统合成重组Ⅶ因子，如BHK细胞、CHO细胞、昆虫细胞和鱼胚胎等。同时，不同外源功能性基因对重组Ⅶ因子活性和翻译后修饰对表达产量的影响也通过不同方法进行探究。本文从Ⅶ因子在凝血途径中的作用、重组表达重组Ⅶ因子和翻译后修饰对合成的影响3个方面对重组Ⅶ因子表达和合成机制研究进行了综述。
혈우병시유우인체내결핍상응응혈인자조성적응혈장애，목전주요이용기인중조기술재불동세포중표체중조응혈인자Ⅶ（Ⅶ인자）종이대혈우환자출혈진행치료。위유효제고중조Ⅶ인자적산량급활성，이유연구자상시이용불동진핵표체계통합성중조Ⅶ인자，여BHK세포、CHO세포、곤충세포화어배태등。동시，불동외원공능성기인대중조Ⅶ인자활성화번역후수식대표체산량적영향야통과불동방법진행탐구。본문종Ⅶ인자재응혈도경중적작용、중조표체중조Ⅶ인자화번역후수식대합성적영향3개방면대중조Ⅶ인자표체화합성궤제연구진행료종술。
Haemophilia is caused by lack of coagulation factor VIII or IX in patients′blood with inadequate hemostasis.Currently recombinant coagulation factor VII(rFVII)produced in different cells is used against clini-cal bleeding of haemophilia patients.To enhance the production and activity of rFVII;some eukaryotic cells such as baby hamster kidney(BHK);Chinese hamster ovary(CHO);insect cell and fish embryo;were used to express rFVII.Meanwhile;the effect of functional gene on the activity of rFVII and the limitation of rFVII production caused by post-translational modification were investigated by different methods.The role of rFVII in hemostasis;synthesis of rFVII in different eukaryotic cells and impact on production of post-translational modification are reviewed in this article.