中国药科大学学报
中國藥科大學學報
중국약과대학학보
Journal of China Pharmaceutical University
2015年
5期
600-604
,共5页
姜玉秋%唐桥斐%张爽%闫智永
薑玉鞦%唐橋斐%張爽%閆智永
강옥추%당교비%장상%염지영
慢性鼻-鼻窦炎%没食子酸%环丙沙星%铜绿假单胞杆菌%联合用药
慢性鼻-鼻竇炎%沒食子痠%環丙沙星%銅綠假單胞桿菌%聯閤用藥
만성비-비두염%몰식자산%배병사성%동록가단포간균%연합용약
chronic rhinosinusitis%gallic acid%ciprofloxacin%Pseudomonas aeruginosa%drug combination
探讨没食子酸与环丙沙星联用对小鼠慢性鼻-鼻窦炎模型（ CRS）的作用。收集难治性CRS患者鼻黏膜样本，分离铜绿假单胞杆菌（ Pseudomonas aeruginosa）构建小鼠CRS模型，没食子酸（ GA）与环丙沙星（ CIP）单独或联合灌胃治疗， HE染色观察小鼠鼻黏膜病理变化，ELISA检测血清炎性因子TNF-α、IL-6、IL-8的表达、试剂盒检测SOD活性、MDA及ROS含量，Western blot检测鼻黏膜组织中TNF-α、IL-6、IL-8、IκB及NF-κB p65的表达。结果显示，GA与CIP可显著降低CRS小鼠TNF-α、IL-6、IL-8、MDA、ROS及NF-κB p65的含量，增加SOD活性及IκB的表达，且联合用药效果更显著。结果说明，GA与CIP联用治疗CRS效果优于单独用药，可能通过抑制NF-κB信号通路，下调TNF-α、IL-6、IL-8表达发挥作用。
探討沒食子痠與環丙沙星聯用對小鼠慢性鼻-鼻竇炎模型（ CRS）的作用。收集難治性CRS患者鼻黏膜樣本，分離銅綠假單胞桿菌（ Pseudomonas aeruginosa）構建小鼠CRS模型，沒食子痠（ GA）與環丙沙星（ CIP）單獨或聯閤灌胃治療， HE染色觀察小鼠鼻黏膜病理變化，ELISA檢測血清炎性因子TNF-α、IL-6、IL-8的錶達、試劑盒檢測SOD活性、MDA及ROS含量，Western blot檢測鼻黏膜組織中TNF-α、IL-6、IL-8、IκB及NF-κB p65的錶達。結果顯示，GA與CIP可顯著降低CRS小鼠TNF-α、IL-6、IL-8、MDA、ROS及NF-κB p65的含量，增加SOD活性及IκB的錶達，且聯閤用藥效果更顯著。結果說明，GA與CIP聯用治療CRS效果優于單獨用藥，可能通過抑製NF-κB信號通路，下調TNF-α、IL-6、IL-8錶達髮揮作用。
탐토몰식자산여배병사성련용대소서만성비-비두염모형（ CRS）적작용。수집난치성CRS환자비점막양본，분리동록가단포간균（ Pseudomonas aeruginosa）구건소서CRS모형，몰식자산（ GA）여배병사성（ CIP）단독혹연합관위치료， HE염색관찰소서비점막병리변화，ELISA검측혈청염성인자TNF-α、IL-6、IL-8적표체、시제합검측SOD활성、MDA급ROS함량，Western blot검측비점막조직중TNF-α、IL-6、IL-8、IκB급NF-κB p65적표체。결과현시，GA여CIP가현저강저CRS소서TNF-α、IL-6、IL-8、MDA、ROS급NF-κB p65적함량，증가SOD활성급IκB적표체，차연합용약효과경현저。결과설명，GA여CIP련용치료CRS효과우우단독용약，가능통과억제NF-κB신호통로，하조TNF-α、IL-6、IL-8표체발휘작용。
This study aimed to detect the combined effects of gallic acid (GA)and ciprofloxacin (CIP)on the murine chronic rhinosinusitis(CRS)model in mice.Pseudomonas aeruginosa from refractory CRS nasal samples were isolated and a CRS model in mice was induced.GA and CIP were intragastrically administered singly or in combination.The nasal histopathologic change was observed by hematoxylin and eosin (HE)staining.The concentration of TNF-α;IL-6 and IL-8 in serum were determined by ELISA assay.The activity of SOD and contents of MDA and ROS were measured with commercially available kits.The expressions of IκB;NF-κB p65;TNF-α;IL-6 and IL-8 in nasal mucosa tissues were measured by Western blotting assay.The results showed that the inflammation of CRS in each treatment group was significantly attenuated.The expression level of TNF-α;IL-6;IL-8;MDA;NF-κB p65 and the contents of ROS were reduced significantly in treated groups;while the activity of SOD and the expression level of IκB were increased.More obvious effects were achieved in CA and CIP combined group.The data showed that combination of GA and CIP was superior to GA or CIP alone;and the combined therapy might be related with inhibiting NF-κB signaling pathway and downregulating the expressions of TNF-α;IL-6 and IL-8.
