CAJ | 학술논문

以人脐静脉内皮细胞（HUVEC）为模拟肿瘤血管内皮细胞的实验模型，研究combretastatin A-4（CA-4）的氨基糖类衍生物CPU-XT-008对HUVEC的增殖、周期分布、微管蛋白聚合以及关键周期调控蛋白表达的影响。采用MTT法检测CPU-XT-008对HUVEC增殖的影响；采用流式细胞仪检测CPU-XT-008对HUVEC周期分布的影响；采用免疫荧光染色检测CPU-XT-008对HUVEC凋亡及β-tubulin微管蛋白的聚合变化；采用Western blot检测CPU-XT-008对周期蛋白Cyclin B1，Cdc2和微管蛋白β-tubulin的影响。结果表明，CPU-XT-008能以微管蛋白为靶点抑制β-tubulin微管蛋白聚合，通过上调HUVEC的细胞周期蛋白Cyclin B1水平表达，下调细胞周期依赖性激酶Cdc2蛋白水平的表达从而引发细胞G2／M期阻滞，抑制细胞增殖并诱导细胞凋亡。
이인제정맥내피세포（HUVEC）위모의종류혈관내피세포적실험모형，연구combretastatin A-4（CA-4）적안기당류연생물CPU-XT-008대HUVEC적증식、주기분포、미관단백취합이급관건주기조공단백표체적영향。채용MTT법검측CPU-XT-008대HUVEC증식적영향；채용류식세포의검측CPU-XT-008대HUVEC주기분포적영향；채용면역형광염색검측CPU-XT-008대HUVEC조망급β-tubulin미관단백적취합변화；채용Western blot검측CPU-XT-008대주기단백Cyclin B1，Cdc2화미관단백β-tubulin적영향。결과표명，CPU-XT-008능이미관단백위파점억제β-tubulin미관단백취합，통과상조HUVEC적세포주기단백Cyclin B1수평표체，하조세포주기의뢰성격매Cdc2단백수평적표체종이인발세포G2／M기조체，억제세포증식병유도세포조망。
Taking human umbilical vein endothelial cells (HUVEC)as experimental model which can simulate tumor-derived vascular endothelial cells;the effects of CPU-XT-008;an amino sugar derivative of combretastatin A-4 (CA-4);on HUVEC proliferation;cell cycle distribution;tubulin polymerization and the key regulatory pro-tein of cell cycle were studied.The effect of CPU-XT-008 on the proliferation of HUVEC was determined by MTT assay.The cell cycle distribution caused by CPU-XT-008 was detected by flow cytometry.Immunofluorescence was used to detect apoptosis and tubulin polymerization.The expressions of Cyclin B1;Cdc2 and β-tubulin were detected by Western blotting.Results demonstrated that CPU-XT-008 could target tubulin and inhibit the poly-merization ofβ-tubulin;and it could lead to G2/M cell cycle arrest in HUVEC by up-regulating Cyclin B1 expres-sion and down-regulating Cdc2 and p-Cdc2 expression;which resulted in inhibiting the proliferation of HUVEC and inducing its apoptosis.