CAJ | 학술논문

目的：比较分析长链非编码RNA( lncRNA)在乳腺癌组织与外周血中的表达情况,筛选出参与乳腺癌发生、复发及转移过程中重要的lncRNA。方法利用芯片技术分别检测并筛选乳腺癌组织与外周血差异表达的lncRNA及mRNA,应用生物信息学方法,包括基因本体论( gene ontology, GO)和Pathway分析,分析乳腺癌组织与外周血中lncRNA及mRNA的表达谱。分析得到乳腺癌外周血与癌组织中共同表达与特异表达的lncRNA,并且进一步分析差异基因可能调控的相关通路,进而发现参与乳腺癌发生发展和转移过程的lncRNAs。结果笔者发现在乳腺癌组织和外周血中共同上调的lncRNA有7条(包括RP11-707G14.7、RP11-771K4.1、XLOC 013658、RP11-201A3.1、RP11-298H24.1、BM151951、XLOC 011403),共同下调的lncRNA有12条(LOC100506035、PWRN1、MAGI2-AS3、RP11-67L3.2、CTC-454M9.1、AC017006.2、AC002456.2、RP11-430B1.2、RP11-945C19.4、RP11-839D17.3、CTA-407F11.8、LINC00420)。 GO和Pathway分析结果表明在乳腺癌患者外周血和组织中均差异表达的基因主要参与调控免疫反应与TGF-β通路等。结论 lncRNA在乳腺癌组织和外周血中呈差异表达,可能参与到乳腺癌的发生、发展以及复发转移中,有可能成为临床诊断与治疗乳腺癌的新靶点。
목적：비교분석장련비편마RNA( lncRNA)재유선암조직여외주혈중적표체정황,사선출삼여유선암발생、복발급전이과정중중요적lncRNA。방법이용심편기술분별검측병사선유선암조직여외주혈차이표체적lncRNA급mRNA,응용생물신식학방법,포괄기인본체론( gene ontology, GO)화Pathway분석,분석유선암조직여외주혈중lncRNA급mRNA적표체보。분석득도유선암외주혈여암조직중공동표체여특이표체적lncRNA,병차진일보분석차이기인가능조공적상관통로,진이발현삼여유선암발생발전화전이과정적lncRNAs。결과필자발현재유선암조직화외주혈중공동상조적lncRNA유7조(포괄RP11-707G14.7、RP11-771K4.1、XLOC 013658、RP11-201A3.1、RP11-298H24.1、BM151951、XLOC 011403),공동하조적lncRNA유12조(LOC100506035、PWRN1、MAGI2-AS3、RP11-67L3.2、CTC-454M9.1、AC017006.2、AC002456.2、RP11-430B1.2、RP11-945C19.4、RP11-839D17.3、CTA-407F11.8、LINC00420)。 GO화Pathway분석결과표명재유선암환자외주혈화조직중균차이표체적기인주요삼여조공면역반응여TGF-β통로등。결론 lncRNA재유선암조직화외주혈중정차이표체,가능삼여도유선암적발생、발전이급복발전이중,유가능성위림상진단여치료유선암적신파점。
Objective To compare the expression of long non-coding RNA ( lncRNA ) in both peripheral blood and carcinoma tissues of breast cancer patients and reveal the important lncRNAs that may participating in the development, recurrence and metastasis of breast cancer. Methods Microarray chip was used to detect and screen the differentially expressed lncRNA and mRNA in carcinoma tissue and peripheral blood of breast cancer patients. lncRNA and mRNA expression profiles were analyzed by bioinformatics tools, including gene ontology ( GO ) and Pathway analysis. The differentially expressed and commonly expressed lncRNAs both in peripheral blood and carcinoma tissues were detected. Moreover, we further analyzed the relevant pathways regulated by differentially expressed genes, as well as lncRNAs participating in the development and metastasis of breast cancer. Results There were 7 up-regulated lncRNAs ( including RP11-707G14. 7,RP11-771K4. 1,XLOC 013658,RP11-201A3. 1,RP11-298H24. 1,BM151951,XLOC 011403) and 12 down-regulated lncRNAs in both carcinoma tissue and peripheral blood ( including LOC100506035, PWRN1, MAGI2-AS3, RP11-67L3. 2, CTC-454M9. 1, AC017006. 2, AC002456. 2, RP11-430B1. 2, RP11-945C19. 4, RP11-839D17. 3, CTA-407F11. 8, LINC00420 ). GO and Pathway analysis indicated that differentially expressed genes in both peripheral blood and carcinoma tissues of breast cancer patients were mainly involved in the regulation of immune response and TGF-β pathway. Conclusion lncRNAs were differentially expressed genes in both peripheral blood and carcinoma tissues of breast cancer patients, which may be involved in the development, recurrence and metastasis of breast cancer, regarded as possible potential targets for clinical diagnosis and treatment.